Down-regulated Genes in Mouse Dental Papillae and Pulp

• Published in: Journal of dental research Vol. 89; no. 7; pp. 679 - 683
• Main Authors: Sasaki, H., Muramatsu, T., Kwon, H.-J., Yamamoto, H., Hashimoto, S., Jung, H.-S., Shimono, M.
• Format: Journal Article
• Language: English
• Published: Los Angeles, CA SAGE Publications 01.07.2010; International Association for Dental Research; SAGE PUBLICATIONS, INC
• Subjects: ADAM Proteins - analysis; ADAM Proteins - genetics; ADAMTS4 Protein; Aldehyde Dehydrogenase - analysis; Aldehyde Dehydrogenase - genetics; Animals; Biological and medical sciences; Cell Death - genetics; Dental Papilla - cytology; Dental Papilla - embryology; Dental Pulp - cytology; Dental Pulp - embryology; Development. Metamorphosis. Moult. Ageing; Down-Regulation - genetics; Epithelial Cells - cytology; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation, Developmental - genetics; In Situ Hybridization; Lymphoid Enhancer-Binding Factor 1 - analysis; Lymphoid Enhancer-Binding Factor 1 - genetics; Mice; Mice, Inbred ICR; Mouth. Exocrine and endocrine salivary glands. Teeth. Esophagus; Odontoblasts - cytology; Odontogenesis - genetics; Oligonucleotide Array Sequence Analysis; Polymerase Chain Reaction; Procollagen N-Endopeptidase - analysis; Procollagen N-Endopeptidase - genetics; Tooth Calcification - genetics; Tooth Germ - cytology; Tooth Germ - embryology; Vertebrates: anatomy and physiology, studies on body, several organs or systems; Vertebrates: digestive system; dental pulp; tooth morphogenesis; odontogenesis; gene expression; dental papilla; Embryonic development; Embryo; Rodentia; Teeth; Gene expression; Dental papilla; Postnatal development; Young animal; Odontogenesis; Vertebrata; Regulation(control); Mammalia; Gene; Mouse; Physiology; Tooth; Dental pulp
• ISSN: 0022-0345; 1544-0591; 1544-0591
• DOI: 10.1177/0022034510366844

Important factors involved in odontogenesis in mouse dental papillae disappear between the pre- and post-natal stages of development. Therefore, we hypothesized that certain genes involved in odontogenesis in dental papillae were subject to pre-/post-natal down-regulation. Our goal was to identify, by microarray analysis, which genes were down-regulated. Dental papillae were isolated from embryonic 16-day-, 18-day- (E16, E18), and post-natal 3-day-old (P3) murine first mandibular molar germs and analyzed by microarray. The number of down-regulated genes was 2269 between E16 and E18, and 3130 between E18 and P3. Drastic down-regulation (fold change > 10.0) of Adamts4, Aldha1a2, and Lef1 was observed at both E16 and E18, and quantitative RT-PCR revealed a post-natal reduction in their expression (Adamts4, 1/3; Aldh1a2, 1/13; and Lef1, 1/37). These results suggest that down-regulation of these three genes is an important factor in normal odontogenesis in dental papillae.