Comprehensive N-glycosylation mapping of envelope glycoprotein from tick-borne encephalitis virus grown in human and tick cells

• Published in: Scientific reports Vol. 10; no. 1; p. 13204
• Main Authors: Lattová, Erika, Straková, Petra, Pokorná-Formanová, Petra, Grubhoffer, Libor, Bell-Sakyi, Lesley, Zdráhal, Zbyněk, Palus, Martin, Ruzek, Daniel
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 06.08.2020; Nature Publishing Group
• Subjects: 631/326/596; 631/92/221; Amino Acid Sequence; Animals; Antibiotics; Antiviral agents; Antiviral drugs; Arachnids; Asparagine; Cell Line, Tumor; Crystallography; Encephalitis; Encephalitis Viruses, Tick-Borne - growth & development; Encephalitis Viruses, Tick-Borne - metabolism; Glycopeptides; Glycoproteins; Glycoproteins - chemistry; Glycoproteins - metabolism; Glycosylation; Humanities and Social Sciences; Humans; Insect bites; Mammalian cells; Mannose; multidisciplinary; N-glycans; Oligosaccharides; Polysaccharides; Protein structure; Proteins; Science; Science (multidisciplinary); Tick-borne encephalitis; Ticks - virology; Viral envelope proteins; Viral Envelope Proteins - chemistry; Viral Envelope Proteins - metabolism

Tick-borne encephalitis virus (TBEV) is the causative agent of severe human neuroinfections that most commonly occur after a tick bite. N -Glycosylation of the TBEV envelope (E) glycoprotein is critical for virus egress in mammalian cells, but not in tick cells. In addition, glycans have been report...