Differential trafficking and timed localization of two chitin synthase proteins, Chs2p and Chs3p

• Published in: The Journal of cell biology Vol. 135; no. 3; pp. 597 - 610
• Main Authors: Chuang, J.S. (University of California, Berkeley.), Schekman, R.W
• Format: Journal Article
• Language: English
• Published: United States Rockefeller University Press 01.11.1996; The Rockefeller University Press
• Subjects: ABSORCION; ABSORPTION; analysis; Aspartic Acid Endopeptidases; Aspartic Acid Endopeptidases - metabolism; Biological Transport; biosynthesis; carbohydrate metabolism; Cell Cycle; Cell division; Cell membranes; Cell walls; Cells; Cellular biology; Chitin; Chitin - biosynthesis; chitin synthase; Chitin Synthase - analysis; Chitin Synthase - biosynthesis; Chitin Synthase - genetics; Chitin Synthase - metabolism; CHITINE; DEGRADACION; DEGRADATION; DIVISION CELLULAIRE; DIVISION CELULAR; Endocytosis; endoplasmic reticulum; Endoplasmic Reticulum - metabolism; enzymology; Epitopes; Epitopes - analysis; ESTRUCTURA CELULAR; Fungal Proteins; Fungal Proteins - physiology; genetics; GLICOSILTRANSFERASAS; GLYCOSYLTRANSFERASE; growth & development; immunocytochemistry; IMMUNOLOGIE; INMUNOLOGIA; metabolism; METABOLISME DES GLUCIDES; METABOLISMO DE CARBOHIDRATOS; Munc18 Proteins; MUTACION; MUTATION; Nerve Tissue Proteins; Nerve Tissue Proteins - physiology; ORGANITE CELLULAIRE; ORGANULOS CITOPLASMICOS; PARED CELULAR; PAROI CELLULAIRE; Perceptual localization; physiology; plasma membrane; PROTEASAS; PROTEASE; protein degradation; protein secretion; protein synthesis; PROTEINAS; proteinases; PROTEINE; Proteins; QUITINA; Recombinant Fusion Proteins; Recombinant Fusion Proteins - metabolism; RETICULO ENDOPLASMATICO; RETICULUM ENDOPLASMIQUE; SACCHAROMYCES CEREVISIAE; Saccharomyces cerevisiae - enzymology; Saccharomyces cerevisiae - genetics; Saccharomyces cerevisiae - growth & development; Saccharomyces cerevisiae Proteins; SECRECION; SECRETION; SINTESIS DE PROTEINAS; STRUCTURE CELLULAIRE; SYNTHESE PROTEIQUE; Temperature; VACUOLA; VACUOLE; Vacuoles; Vacuoles - metabolism; Vesicular Transport Proteins; Yeast; Yeasts

The deposition of the polysaccharide chitin in the Saccharomyces cerevisiae cell wall is temporally and spatially regulated. Chitin synthase III (Chs3p) synthesizes a ring of chitin at the onset of bud emergence, marking the base of the incipient bud. At the end of mitosis, chitin synthase II (Chs2p...