Abundance of cell‐free mitochondrial DNA in spent culture medium associated with morphokinetics and blastocyst collapse of expanded blastocysts

• Published in: Reproductive medicine and biology Vol. 19; no. 4; pp. 404 - 414
• Main Authors: Kobayashi, Mitsuru, Kobayashi, Junichi, Shirasuna, Koumei, Iwata, Hisataka
• Format: Journal Article
• Language: English
• Published: Japan John Wiley & Sons, Inc 01.10.2020; John Wiley and Sons Inc; Wiley
• Subjects: blastocyst collapse; Blastocysts; Cell culture; Cell cycle; cell‐free DNA; Culture media; Deoxyribonucleic acid; DNA; embryo selection; Embryonic development; Embryos; Genetic research; Genetic testing; Genomes; Hormone replacement therapy; In vitro fertilization; Microscopy; mitochondria; Mitochondrial DNA; Morphology; Motility; Original; Ovaries; Pregnancy; Sperm; time‐lapse incubator; Ultrasonic imaging; time‐lapse incubator; blastocyst collapse; embryo selection; mitochondria; cell‐free DNA

Purpose This retrospective observational study investigated relationships between the abundance of cell‐free mitochondrial DNA (cf‐mtDNA) in spent culture medium (SCM) of human‐expanded blastocysts and their morphokinetics to address the question of whether the abundance of cf‐mtDNA in SCM could pre...