Abundance of cell‐free mitochondrial DNA in spent culture medium associated with morphokinetics and blastocyst collapse of expanded blastocysts

Purpose This retrospective observational study investigated relationships between the abundance of cell‐free mitochondrial DNA (cf‐mtDNA) in spent culture medium (SCM) of human‐expanded blastocysts and their morphokinetics to address the question of whether the abundance of cf‐mtDNA in SCM could pre...

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Published inReproductive medicine and biology Vol. 19; no. 4; pp. 404 - 414
Main Authors Kobayashi, Mitsuru, Kobayashi, Junichi, Shirasuna, Koumei, Iwata, Hisataka
Format Journal Article
LanguageEnglish
Published Japan John Wiley & Sons, Inc 01.10.2020
John Wiley and Sons Inc
Wiley
Subjects
blastocyst collapse
Blastocysts
Cell culture
Cell cycle
cell‐free DNA
Culture media
Deoxyribonucleic acid
DNA
embryo selection
Embryonic development
Embryos
Genetic research
Genetic testing
Genomes
Hormone replacement therapy
In vitro fertilization
Microscopy
mitochondria
Mitochondrial DNA
Morphology
Motility
Original
Ovaries
Pregnancy
Sperm
time‐lapse incubator
Ultrasonic imaging
time‐lapse incubator
blastocyst collapse
embryo selection
mitochondria
cell‐free DNA
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Abstract Purpose This retrospective observational study investigated relationships between the abundance of cell‐free mitochondrial DNA (cf‐mtDNA) in spent culture medium (SCM) of human‐expanded blastocysts and their morphokinetics to address the question of whether the abundance of cf‐mtDNA in SCM could predict the quality of blastocysts. Methods Embryos (n = 53) were individually cultured in a time‐lapse incubator until they reached the expanded blastocyst stage (5 or 6 days), following which copy numbers of cf‐mtDNA in SCM (20 μL) of expanded blastocysts were determined using real‐time PCR. Results The duration between start of blastulation to expanded blastocyst (tEB–tSB) and between that of the blastocyst stage to expanded blastocyst (tEB–tB) significantly and positively correlated with the abundance of cf‐mtDNA in the SCM (tEB–tSB: r = .46; P < .01; tEB–tB: r = .47; P < .01). The abundance of cf‐mtDNA in the SCM was significantly greater in blastocysts with blastocyst collapse (BC), than without BC, and significantly and positively correlated with the number of BC. Conclusions The abundance of cf‐mtDNA in the SCM was associated with expansion duration and BC. Thus, cf‐mtDNA abundance in the SCM serves as a marker to predict the quality of expanded blastocysts. The abundance of cell free‐mitochondrial DNA in the spent culture medium is associated with expansion duration and presence of blastocyst collapse.
AbstractList This retrospective observational study investigated relationships between the abundance of cell-free mitochondrial DNA (cf-mtDNA) in spent culture medium (SCM) of human-expanded blastocysts and their morphokinetics to address the question of whether the abundance of cf-mtDNA in SCM could predict the quality of blastocysts. Embryos (n = 53) were individually cultured in a time-lapse incubator until they reached the expanded blastocyst stage (5 or 6 days), following which copy numbers of cf-mtDNA in SCM (20 μL) of expanded blastocysts were determined using real-time PCR. The duration between start of blastulation to expanded blastocyst (tEB-tSB) and between that of the blastocyst stage to expanded blastocyst (tEB-tB) significantly and positively correlated with the abundance of cf-mtDNA in the SCM (tEB-tSB: r = .46;  < .01; tEB-tB: r = .47;  < .01). The abundance of cf-mtDNA in the SCM was significantly greater in blastocysts with blastocyst collapse (BC), than without BC, and significantly and positively correlated with the number of BC. The abundance of cf-mtDNA in the SCM was associated with expansion duration and BC. Thus, cf-mtDNA abundance in the SCM serves as a marker to predict the quality of expanded blastocysts.
PurposeThis retrospective observational study investigated relationships between the abundance of cell‐free mitochondrial DNA (cf‐mtDNA) in spent culture medium (SCM) of human‐expanded blastocysts and their morphokinetics to address the question of whether the abundance of cf‐mtDNA in SCM could predict the quality of blastocysts.MethodsEmbryos (n = 53) were individually cultured in a time‐lapse incubator until they reached the expanded blastocyst stage (5 or 6 days), following which copy numbers of cf‐mtDNA in SCM (20 μL) of expanded blastocysts were determined using real‐time PCR.ResultsThe duration between start of blastulation to expanded blastocyst (tEB–tSB) and between that of the blastocyst stage to expanded blastocyst (tEB–tB) significantly and positively correlated with the abundance of cf‐mtDNA in the SCM (tEB–tSB: r = .46; P < .01; tEB–tB: r = .47; P < .01). The abundance of cf‐mtDNA in the SCM was significantly greater in blastocysts with blastocyst collapse (BC), than without BC, and significantly and positively correlated with the number of BC.ConclusionsThe abundance of cf‐mtDNA in the SCM was associated with expansion duration and BC. Thus, cf‐mtDNA abundance in the SCM serves as a marker to predict the quality of expanded blastocysts.
This retrospective observational study investigated relationships between the abundance of cell-free mitochondrial DNA (cf-mtDNA) in spent culture medium (SCM) of human-expanded blastocysts and their morphokinetics to address the question of whether the abundance of cf-mtDNA in SCM could predict the quality of blastocysts.PURPOSEThis retrospective observational study investigated relationships between the abundance of cell-free mitochondrial DNA (cf-mtDNA) in spent culture medium (SCM) of human-expanded blastocysts and their morphokinetics to address the question of whether the abundance of cf-mtDNA in SCM could predict the quality of blastocysts.Embryos (n = 53) were individually cultured in a time-lapse incubator until they reached the expanded blastocyst stage (5 or 6 days), following which copy numbers of cf-mtDNA in SCM (20 μL) of expanded blastocysts were determined using real-time PCR.METHODSEmbryos (n = 53) were individually cultured in a time-lapse incubator until they reached the expanded blastocyst stage (5 or 6 days), following which copy numbers of cf-mtDNA in SCM (20 μL) of expanded blastocysts were determined using real-time PCR.The duration between start of blastulation to expanded blastocyst (tEB-tSB) and between that of the blastocyst stage to expanded blastocyst (tEB-tB) significantly and positively correlated with the abundance of cf-mtDNA in the SCM (tEB-tSB: r = .46; P < .01; tEB-tB: r = .47; P < .01). The abundance of cf-mtDNA in the SCM was significantly greater in blastocysts with blastocyst collapse (BC), than without BC, and significantly and positively correlated with the number of BC.RESULTSThe duration between start of blastulation to expanded blastocyst (tEB-tSB) and between that of the blastocyst stage to expanded blastocyst (tEB-tB) significantly and positively correlated with the abundance of cf-mtDNA in the SCM (tEB-tSB: r = .46; P < .01; tEB-tB: r = .47; P < .01). The abundance of cf-mtDNA in the SCM was significantly greater in blastocysts with blastocyst collapse (BC), than without BC, and significantly and positively correlated with the number of BC.The abundance of cf-mtDNA in the SCM was associated with expansion duration and BC. Thus, cf-mtDNA abundance in the SCM serves as a marker to predict the quality of expanded blastocysts.CONCLUSIONSThe abundance of cf-mtDNA in the SCM was associated with expansion duration and BC. Thus, cf-mtDNA abundance in the SCM serves as a marker to predict the quality of expanded blastocysts.
Purpose This retrospective observational study investigated relationships between the abundance of cell‐free mitochondrial DNA (cf‐mtDNA) in spent culture medium (SCM) of human‐expanded blastocysts and their morphokinetics to address the question of whether the abundance of cf‐mtDNA in SCM could predict the quality of blastocysts. Methods Embryos (n = 53) were individually cultured in a time‐lapse incubator until they reached the expanded blastocyst stage (5 or 6 days), following which copy numbers of cf‐mtDNA in SCM (20 μL) of expanded blastocysts were determined using real‐time PCR. Results The duration between start of blastulation to expanded blastocyst (tEB–tSB) and between that of the blastocyst stage to expanded blastocyst (tEB–tB) significantly and positively correlated with the abundance of cf‐mtDNA in the SCM (tEB–tSB: r = .46; P < .01; tEB–tB: r = .47; P < .01). The abundance of cf‐mtDNA in the SCM was significantly greater in blastocysts with blastocyst collapse (BC), than without BC, and significantly and positively correlated with the number of BC. Conclusions The abundance of cf‐mtDNA in the SCM was associated with expansion duration and BC. Thus, cf‐mtDNA abundance in the SCM serves as a marker to predict the quality of expanded blastocysts. The abundance of cell free‐mitochondrial DNA in the spent culture medium is associated with expansion duration and presence of blastocyst collapse.
The abundance of cell free‐mitochondrial DNA in the spent culture medium is associated with expansion duration and presence of blastocyst collapse.
Purpose: This retrospective observational study investigated relationships between the abundance of cell-free mitochondrial DNA (cf-mtDNA) in spent culture medium (SCM) of human-expanded blastocysts and their morphokinetics to address the question of whether the abundance of cf-mtDNA in SCM could predict the quality of blastocysts. Methods: Embryos (n = 53) were individually cultured in a time-lapse incubator until they reached the expanded blastocyst stage (5 or 6 days), following which copy numbers of cf-mtDNA in SCM (20 μL) of expanded blastocysts were determined using real-time PCR. Results: The duration between start of blastulation to expanded blastocyst (tEB-tSB) and between that of the blastocyst stage to expanded blastocyst (tEB-tB) significantly and positively correlated with the abundance of cf-mtDNA in the SCM (tEB-tSB: r =.46; P <.01; tEB-tB: r =.47; P <.01). The abundance of cf-mtDNA in the SCM was significantly greater in blastocysts with blastocyst collapse (BC), than without BC, and significantly and positively correlated with the number of BC. Conclusions: The abundance of cf-mtDNA in the SCM was associated with expansion duration and BC. Thus, cf-mtDNA abundance in the SCM serves as a marker to predict the quality of expanded blastocysts.
Abstract Purpose This retrospective observational study investigated relationships between the abundance of cell‐free mitochondrial DNA (cf‐mtDNA) in spent culture medium (SCM) of human‐expanded blastocysts and their morphokinetics to address the question of whether the abundance of cf‐mtDNA in SCM could predict the quality of blastocysts. Methods Embryos (n = 53) were individually cultured in a time‐lapse incubator until they reached the expanded blastocyst stage (5 or 6 days), following which copy numbers of cf‐mtDNA in SCM (20 μL) of expanded blastocysts were determined using real‐time PCR. Results The duration between start of blastulation to expanded blastocyst (tEB–tSB) and between that of the blastocyst stage to expanded blastocyst (tEB–tB) significantly and positively correlated with the abundance of cf‐mtDNA in the SCM (tEB–tSB: r = .46; P < .01; tEB–tB: r = .47; P < .01). The abundance of cf‐mtDNA in the SCM was significantly greater in blastocysts with blastocyst collapse (BC), than without BC, and significantly and positively correlated with the number of BC. Conclusions The abundance of cf‐mtDNA in the SCM was associated with expansion duration and BC. Thus, cf‐mtDNA abundance in the SCM serves as a marker to predict the quality of expanded blastocysts.
Audience Academic
Author Kobayashi, Junichi
Shirasuna, Koumei
Kobayashi, Mitsuru
Iwata, Hisataka
AuthorAffiliation 2 Kanagawa Ladies Clinic Yokohama Japan
1 Department of Animal Science Tokyo University of Agriculture Atsugi Japan
AuthorAffiliation_xml – name: 1 Department of Animal Science Tokyo University of Agriculture Atsugi Japan
– name: 2 Kanagawa Ladies Clinic Yokohama Japan
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  givenname: Junichi
  surname: Kobayashi
  fullname: Kobayashi, Junichi
  organization: Kanagawa Ladies Clinic
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  givenname: Koumei
  surname: Shirasuna
  fullname: Shirasuna, Koumei
  organization: Tokyo University of Agriculture
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  givenname: Hisataka
  orcidid: 0000-0001-7238-8997
  surname: Iwata
  fullname: Iwata, Hisataka
  email: h1iwata@nodai.ac.jp
  organization: Tokyo University of Agriculture
BackLink https://www.ncbi.nlm.nih.gov/pubmed/33071643$$D View this record in MEDLINE/PubMed
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Issue 4
Keywords time‐lapse incubator
blastocyst collapse
embryo selection
mitochondria
cell‐free DNA
Language English
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Snippet Purpose This retrospective observational study investigated relationships between the abundance of cell‐free mitochondrial DNA (cf‐mtDNA) in spent culture...
This retrospective observational study investigated relationships between the abundance of cell-free mitochondrial DNA (cf-mtDNA) in spent culture medium (SCM)...
Purpose: This retrospective observational study investigated relationships between the abundance of cell-free mitochondrial DNA (cf-mtDNA) in spent culture...
PurposeThis retrospective observational study investigated relationships between the abundance of cell‐free mitochondrial DNA (cf‐mtDNA) in spent culture...
The abundance of cell free‐mitochondrial DNA in the spent culture medium is associated with expansion duration and presence of blastocyst collapse.
Abstract Purpose This retrospective observational study investigated relationships between the abundance of cell‐free mitochondrial DNA (cf‐mtDNA) in spent...
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SubjectTerms blastocyst collapse
Blastocysts
Cell culture
Cell cycle
cell‐free DNA
Culture media
Deoxyribonucleic acid
DNA
embryo selection
Embryonic development
Embryos
Genetic research
Genetic testing
Genomes
Hormone replacement therapy
In vitro fertilization
Microscopy
mitochondria
Mitochondrial DNA
Morphology
Motility
Original
Ovaries
Pregnancy
Sperm
time‐lapse incubator
Ultrasonic imaging
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Title Abundance of cell‐free mitochondrial DNA in spent culture medium associated with morphokinetics and blastocyst collapse of expanded blastocysts
URI https://onlinelibrary.wiley.com/doi/abs/10.1002%2Frmb2.12344
https://www.ncbi.nlm.nih.gov/pubmed/33071643
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https://www.proquest.com/docview/2452097670
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https://doaj.org/article/f551fcbc0c2f4418a561632f34bb0e76
Volume 19
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