Development of an optimal protocol for molecular profiling of tumor cells in pleural effusions at single‐cell level

• Published in: Cancer science Vol. 112; no. 5; pp. 2006 - 2019
• Main Authors: Nakamura, Ikuko Takeda, Ikegami, Masachika, Hasegawa, Nobuhiko, Hayashi, Takuo, Ueno, Toshihide, Kawazu, Masahito, Yagishita, Shigehiro, Goto, Yasushi, Shinno, Yuki, Kojima, Yuki, Takamochi, Kazuya, Takahashi, Fumiyuki, Takahashi, Kazuhisa, Mano, Hiroyuki, Kohsaka, Shinji
• Format: Journal Article
• Language: English
• Published: England John Wiley & Sons, Inc 01.05.2021; John Wiley and Sons Inc
• Subjects: Adenocarcinoma - drug therapy; Adenocarcinoma - pathology; Adult; Aged; Aged, 80 and over; Anaplastic Lymphoma Kinase - genetics; Antibodies; Antineoplastic Agents - therapeutic use; Automation; Biopsy; Carbazoles - pharmacology; CD45 antigen; Cell fusion; Cell Separation - methods; circulating tumor cells; Crizotinib - therapeutic use; Cytokeratin; DNA - isolation & purification; Drug Resistance, Neoplasm - genetics; Epidermal growth factor receptors; Exons - genetics; Female; floating tumor cells; Gene Amplification; Gene Deletion; Gene Expression Profiling - methods; Genes, erbB-1; Genomes; High-Throughput Nucleotide Sequencing - methods; Humans; Immunomagnetic Separation - methods; Keratins; Leukocyte Common Antigens; Leukocytes; liquid biopsy; Liquid Biopsy - methods; lung adenocarcinoma; Lung cancer; Lung Neoplasms - drug therapy; Lung Neoplasms - pathology; Male; Medicine; Metastases; Microfluidics; Middle Aged; molecular profiling; Mutation; Negative selection; Neoplastic Cells, Circulating; Oncogene Proteins, Fusion - genetics; Original; Peripheral blood; Piperidines - pharmacology; Pleural effusion; Pleural Effusion, Malignant - genetics; Pleural Effusion, Malignant - pathology; Positive selection; Protein Kinase Inhibitors - pharmacology; Silicon; Tumor cells; Tumors; Whole genome sequencing; Germany; floating tumor cells; circulating tumor cells; liquid biopsy; lung adenocarcinoma; molecular profiling

Liquid biopsy analyzes the current status of primary tumors and their metastatic regions. We aimed to develop an optimized protocol for single‐cell sequencing of floating tumor cells (FTCs) in pleural effusion as a laboratory test. FTCs were enriched using a negative selection of white blood cells b...