Development of an optimal protocol for molecular profiling of tumor cells in pleural effusions at single‐cell level - Nakamura, Ikuko Takeda; Ikegami, Masachika; Hasegawa, Nobuhiko; Hayashi, Takuo; Ueno, Toshihide; Kawazu, Masahito; Yagishita, Shigehiro; Goto, Yasushi; Shinno, Yuki; Kojima, Yuki; Takamochi, Kazuya; Takahashi, Fumiyuki; et al. (Cancer science, 2021)
Keywords: Adenocarcinoma - drug therapy; Adenocarcinoma - pathology; Adult; Aged; Aged, 80 and over; Anaplastic Lymphoma Kinase - genetics; Antibodies; Antineoplastic Agents - therapeutic use; Automation; Biopsy; Carbazoles - pharmacology; CD45 antigen; Cell fusion; Cell Separation - methods; circulating tumor cells; Crizotinib - therapeutic use; Cytokeratin; DNA - isolation & purification; Drug Resistance, Neoplasm - genetics; Epidermal growth factor receptors; Exons - genetics; Female; floating tumor cells; Gene Amplification; Gene Deletion; Gene Expression Profiling - methods; Genes, erbB-1; Genomes; High-Throughput Nucleotide Sequencing - methods; Humans; Immunomagnetic Separation - methods; Keratins; Leukocyte Common Antigens; Leukocytes; liquid biopsy; Liquid Biopsy - methods; lung adenocarcinoma; Lung cancer; Lung Neoplasms - drug therapy; Lung Neoplasms - pathology; Male; Medicine; Metastases; Microfluidics; Middle Aged; molecular profiling; Mutation; Negative selection; Neoplastic Cells, Circulating; Oncogene Proteins, Fusion - genetics; Original; Peripheral blood; Piperidines - pharmacology; Pleural effusion; Pleural Effusion, Malignant - genetics; Pleural Effusion, Malignant - pathology; Positive selection; Protein Kinase Inhibitors - pharmacology; Silicon; Tumor cells; Tumors; Whole genome sequencing; Germany; floating tumor cells; circulating tumor cells; liquid biopsy; lung adenocarcinoma; molecular profiling