不同剂量137Csγ-射线辐射后造血干/祖细胞辐射敏感性差异研究
目的探讨不同剂量^137Csγ-射线辐射后不同时间点C57BL/6小鼠造血细胞辐射敏感性的差异。方法采用随机区组法将72只雄性C57BL/6小鼠分为对照组和2、4、6 Gy照射组。照射组给予2、4、6 Gy 137Csγ-射线一次性全身照射,对照组给予假照射。分别于受照后14 d、35 d和56 d断颈处死小鼠,取外周血进行血象测定,取骨髓细胞测定有核细胞（BMNCs）、造血干细胞（HSCs）和造血祖细胞（HPCs）数目。结果与对照组相比,照射组小鼠造血系统受到不同程度损伤。外周血白细胞计数（WBC）、红细胞数（RBC）、血小板（PLT）和血红蛋白含量（HGB）均有不同程度的降低,其中HGB对...
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| Published in | 天津医药 Vol. 44; no. 3; pp. 314 - 317 |
|---|---|
| Main Author | |
| Format | Journal Article |
| Language | Chinese |
| Published |
中国医学科学院北京协和医学院放射医学研究所,天津市放射医学与分子核医学重点实验室 邮编,300192%中国医学科学院实验动物研究所
2016
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| Subjects | |
| Online Access | Get full text |
| ISSN | 0253-9896 |
| DOI | 10.11958/20150115 |
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| Abstract | 目的探讨不同剂量^137Csγ-射线辐射后不同时间点C57BL/6小鼠造血细胞辐射敏感性的差异。方法采用随机区组法将72只雄性C57BL/6小鼠分为对照组和2、4、6 Gy照射组。照射组给予2、4、6 Gy 137Csγ-射线一次性全身照射,对照组给予假照射。分别于受照后14 d、35 d和56 d断颈处死小鼠,取外周血进行血象测定,取骨髓细胞测定有核细胞（BMNCs）、造血干细胞（HSCs）和造血祖细胞（HPCs）数目。结果与对照组相比,照射组小鼠造血系统受到不同程度损伤。外周血白细胞计数（WBC）、红细胞数（RBC）、血小板（PLT）和血红蛋白含量（HGB）均有不同程度的降低,其中HGB对辐射较不敏感,RBC在受照后35 d已基本恢复至正常值,WBC和PLT对辐射较敏感;6Gy照射对WBC和PLT影响较重,恢复缓慢。照射后14 d,照射组BMNCs、HSCs和HPCs数目均低于对照组;照射后35 d和56 d,6 Gy照射组BMNCs和HPCs数目均低于对照组（均P〈0.05）,2、4 Gy照射组BMNCs和HPCs数目与对照组差异无统计学意义。照射后35 d,照射组HSCs数目均低于对照组;照射后56 d,4、6 Gy照射组HSCs数目均低于对照组（均P〈0.05）,2 Gy照射组HSCs数目与对照组差异无统计学意义。结论不同剂量137Csγ-射线辐射对小鼠造血系统造成不同程度的损伤,且辐射对HSCs的损伤是持久性的。 |
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| AbstractList | R331.2; 目的：探讨不同剂量137Csγ-射线辐射后不同时间点C57BL/6小鼠造血细胞辐射敏感性的差异。方法采用随机区组法将72只雄性C57BL/6小鼠分为对照组和2、4、6 Gy照射组。照射组给予2、4、6 Gy137Csγ-射线一次性全身照射，对照组给予假照射。分别于受照后14 d、35 d和56 d断颈处死小鼠，取外周血进行血象测定，取骨髓细胞测定有核细胞（BMNCs）、造血干细胞（HSCs）和造血祖细胞（HPCs）数目。结果与对照组相比，照射组小鼠造血系统受到不同程度损伤。外周血白细胞计数（WBC）、红细胞数（RBC）、血小板（PLT）和血红蛋白含量（HGB）均有不同程度的降低，其中HGB对辐射较不敏感，RBC在受照后35 d已基本恢复至正常值，WBC和PLT对辐射较敏感；6 Gy照射对WBC和PLT影响较重，恢复缓慢。照射后14 d，照射组BMNCs、HSCs和HPCs数目均低于对照组；照射后35 d和56 d，6 Gy照射组BMNCs和HPCs数目均低于对照组（均P<0.05），2、4 Gy照射组BMNCs和HPCs数目与对照组差异无统计学意义。照射后35 d，照射组HSCs数目均低于对照组；照射后56 d，4、6 Gy照射组HSCs数目均低于对照组（均P<0.05），2 Gy照射组HSCs数目与对照组差异无统计学意义。结论不同剂量137Csγ-射线辐射对小鼠造血系统造成不同程度的损伤，且辐射对HSCs的损伤是持久性的。 目的探讨不同剂量^137Csγ-射线辐射后不同时间点C57BL/6小鼠造血细胞辐射敏感性的差异。方法采用随机区组法将72只雄性C57BL/6小鼠分为对照组和2、4、6 Gy照射组。照射组给予2、4、6 Gy 137Csγ-射线一次性全身照射,对照组给予假照射。分别于受照后14 d、35 d和56 d断颈处死小鼠,取外周血进行血象测定,取骨髓细胞测定有核细胞（BMNCs）、造血干细胞（HSCs）和造血祖细胞（HPCs）数目。结果与对照组相比,照射组小鼠造血系统受到不同程度损伤。外周血白细胞计数（WBC）、红细胞数（RBC）、血小板（PLT）和血红蛋白含量（HGB）均有不同程度的降低,其中HGB对辐射较不敏感,RBC在受照后35 d已基本恢复至正常值,WBC和PLT对辐射较敏感;6Gy照射对WBC和PLT影响较重,恢复缓慢。照射后14 d,照射组BMNCs、HSCs和HPCs数目均低于对照组;照射后35 d和56 d,6 Gy照射组BMNCs和HPCs数目均低于对照组（均P〈0.05）,2、4 Gy照射组BMNCs和HPCs数目与对照组差异无统计学意义。照射后35 d,照射组HSCs数目均低于对照组;照射后56 d,4、6 Gy照射组HSCs数目均低于对照组（均P〈0.05）,2 Gy照射组HSCs数目与对照组差异无统计学意义。结论不同剂量137Csγ-射线辐射对小鼠造血系统造成不同程度的损伤,且辐射对HSCs的损伤是持久性的。 |
| Abstract_FL | Objective To observe the different radiosensitivity induced by different doses of 137Csγ-ray irradiation between hematopoietic stem and progenitor cells. Methods Seventy-two C57BL/6 mice were randomly divided into control group and irradiated groups (2, 4 and 6 Csγ-ray irradiation, n=18 for each group). Mice of control group received sham irradiation, and the rest accepted 2, 4 and 6 Gy137Csγtotal body irradiation, respectively. After 14-day, 35-day and 56-day irradiation, the peripheral blood samples were collected by balls enucleation. The number of bone marrow nuclear cells, hematopoietic stem and progenitor cells were counted. Results The peripheral blood of irradiated mice showed significant changes in the number of white blood cells (WBC), red blood cells (RBC), platelets (PLT) and hemoglobin (HGB) in a dose-response relationship. Compared with the control group, the numbers of BMNCs and hematopoietic progenitor cells (HPCs) were significantly lower in irradiated group. At 35 d and 56 d after 6 Gy irradiation the numbers of BMNCs and HPCs were significantly lower than those of control group (P<0.05). There were no significant differences in numbers of BMNCs and HPCs between irradiated groups (2 and 4 Gy) and control group. The number of bone marrow hematopoietic stem cells (HSCs) was significantly lower in irradiated group than that in control group after 14-d and 56-d irradiation (P<0.05). Conclusion 137Csγ-ray irradiation has some damage in mouse hematopoietic system. The damage caused by radiation is persistent to hematopoietic stem cells. |
| Author | 路璐 李德冠 张俊伶 王月英 孟爱民 |
| AuthorAffiliation | 中国医学科学院北京协和医学院放射医学研究所,天津市放射医学与分子核医学重点实验室,300192 中罔医学科学院实验动物研究所 |
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| Author_FL | LI Deguan ZHANG Junling MENG Aimin LU Lu WANG Yueying |
| Author_FL_xml | – sequence: 1 fullname: LU Lu – sequence: 2 fullname: LI Deguan – sequence: 3 fullname: ZHANG Junling – sequence: 4 fullname: WANG Yueying – sequence: 5 fullname: MENG Aimin |
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| DOI | 10.11958/20150115 |
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| DocumentTitleAlternate | The difference in the radiosensitivity between hematopoietic stem and progenitor cells after different doses of ~（137）Csγ-radiation |
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| Keywords | γ射线 外周血 造血干细胞 γ-ray hematopoietic stem cell hematopoietic progenitor cell peripheral blood 造血祖细胞 137Cs |
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| Notes | γ-ray; peripheral blood; hematopoietic stem cell; hematopoietic progenitor cell; 137Cs LU Lu, LI Deguan, ZHANG Junling, WANG Yueying, MENG Aimin（ 1 Institute of Radiation Medicine, Chinese Academy of Medical Science and Peking Union Medical College, Tianfin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Tianjin 300192, China ; 2 Institute of Laboratory Animal Science, Chinese Academy of Medical Science and Peking Union Medical College） Objective To observe the different radiosensitivity induced by different doses of ~（137）Csγ-ray irradiation betweenhematopoietic stem and progenitor cells. Methods Seventy-two C57BL/6 mice were randomly divided into control group andirradiated groups（2, 4 and 6 Csγ-ray irradiation, n=18 for each group）. Mice of control group received sham irradiation, and therest accepted 2, 4 and 6 Gy ~（137）Csγ total body irradiation, respectively. After 14-day, 35-day and 56-day irradiation, the peripheralblood samples were collected by balls enucleation. The number of bon |
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| Publisher | 中国医学科学院北京协和医学院放射医学研究所,天津市放射医学与分子核医学重点实验室 邮编,300192%中国医学科学院实验动物研究所 |
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| Snippet | 目的探讨不同剂量^137Csγ-射线辐射后不同时间点C57BL/6小鼠造血细胞辐射敏感性的差异。方法采用随机区组法将72只雄性C57BL/6小鼠分为对照组和2、4、6 Gy照射组。照射组给予2、4、6 Gy 137Csγ-射线一次性全身照射,对照组给予假照射。分别于受照后14 d、35 d和56... R331.2; 目的：探讨不同剂量137Csγ-射线辐射后不同时间点C57BL/6小鼠造血细胞辐射敏感性的差异。方法采用随机区组法将72只雄性C57BL/6小鼠分为对照组和2、4、6 Gy照射组。照射组给予2、4、6 Gy137Csγ-射线一次性全身照射，对照组给予假照射。分别于受照后14 d、35 d和56... |
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| StartPage | 314 |
| SubjectTerms | 137Cs γ射线 外周血 造血干细胞 造血祖细胞 |
| Title | 不同剂量137Csγ-射线辐射后造血干/祖细胞辐射敏感性差异研究 |
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