罗非鱼无乳链球菌LrrG-Sip融合蛋白免疫原性研究
为探究无乳链球菌LrrG（Leucine—rich repeat protein from GBS）和表面免疫原性蛋白Sip（surface immunogenic protein）串联表达的LrrG—Sip重组融合蛋白的免疫原性，该研究将原核表达的LrrG—Sip重组融合蛋白分别以0．5μg·g-1（R1组）、1．0μg·g-1（R2组）和1．5μg·g-1（R3组）每尾200μL腹腔注射免疫尼罗罗非鱼（Oreochromis niloticus），同时以Sip蛋白（s组）、LrrG蛋白（L组）以及PBS（P组）作为对照。2周后对所有免疫鱼体进行无乳链球菌（Streptococcus aga...
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| Published in | 南方水产科学 Vol. 13; no. 3; pp. 51 - 57 |
|---|---|
| Main Author | |
| Format | Journal Article |
| Language | Chinese |
| Published |
中国水产科学研究院珠江水产研究所,农业部热带亚热带水产资源利用与养殖重点实验室,广东 广州 510380
2017
上海海洋大学水产与生命学院,上海 201306%中国水产科学研究院珠江水产研究所,农业部热带亚热带水产资源利用与养殖重点实验室,广东 广州 510380 |
| Subjects | |
| Online Access | Get full text |
| ISSN | 2095-0780 |
| DOI | 10.3969/j.issn.2095-0780.2017.03.007 |
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| Abstract | 为探究无乳链球菌LrrG（Leucine—rich repeat protein from GBS）和表面免疫原性蛋白Sip（surface immunogenic protein）串联表达的LrrG—Sip重组融合蛋白的免疫原性，该研究将原核表达的LrrG—Sip重组融合蛋白分别以0．5μg·g-1（R1组）、1．0μg·g-1（R2组）和1．5μg·g-1（R3组）每尾200μL腹腔注射免疫尼罗罗非鱼（Oreochromis niloticus），同时以Sip蛋白（s组）、LrrG蛋白（L组）以及PBS（P组）作为对照。2周后对所有免疫鱼体进行无乳链球菌（Streptococcus agalactiae）人工攻毒，攻毒剂量为其半致死浓度（LD50：4．0×10^8CFU·mL-1）。结果显示LrG—Sip重组融合蛋白R1组对尼罗罗非鱼的相对免疫保护率最高，达89．14％；且免疫后第14和第28天，该组鱼体血清抗体OD450nm值分别达0．63和0．64，均显著高于单一蛋白对照组（S和L组）和PBS组（P〈0．05）；R1组鱼体血清过氧化物酶（POD）和碱性磷酸酶（AKP）活性在上述2个时间点也显著高于其他组（P〈0．05）；但溶菌酶（LZM）和总超氧化物歧化酶（T—SOD）活性与其他组之间差异不显著（P〉0．05）。初步表明LrrG-Sip重组融合蛋白具有良好的免疫原性，其免疫原性明显优于单个蛋白，且能有效减少免疫剂量。 |
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| AbstractList | S924.5; 为探究无乳链球菌LrrG(Leucine-rich repeat protein from GBS)和表面免疫原性蛋白Sip(surface immunogenic protein)串联表达的LrrG-Sip重组融合蛋白的免疫原性,该研究将原核表达的LrrG-Sip重组融合蛋白分别以0.5 μg·g-1(R1组)、1.0 μg·g-1(R2组)和1.5 μg·g-1(R3组)每尾200 μL腹腔注射免疫尼罗罗非鱼(Oreochromis niloticus),同时以Sip蛋白(S组)、LrrG蛋白(L组)以及PBS(P组)作为对照.2周后对所有免疫鱼体进行无乳链球菌(Streptococcus agalactiae)人工攻毒,攻毒剂量为其半致死浓度(LD50:4.0×108 CFU·mL-1).结果显示LrrG-Sip重组融合蛋白R1组对尼罗罗非鱼的相对免疫保护率最高,达89.14%;且免疫后第14和第28天,该组鱼体血清抗体OD450 nm值分别达0.63和0.64,均显著高于单一蛋白对照组(S和L组)和PBS组(P<0.05);R1组鱼体血清过氧化物酶(POD)和碱性磷酸酶(AKP)活性在上述2个时间点也显著高于其他组(P<0.05);但溶菌酶(LZM)和总超氧化物歧化酶(T-SOD)活性与其他组之间差异不显著(P>0.05).初步表明LrrG-Sip重组融合蛋白具有良好的免疫原性,其免疫原性明显优于单个蛋白,且能有效减少免疫剂量. 为探究无乳链球菌LrrG（Leucine—rich repeat protein from GBS）和表面免疫原性蛋白Sip（surface immunogenic protein）串联表达的LrrG—Sip重组融合蛋白的免疫原性，该研究将原核表达的LrrG—Sip重组融合蛋白分别以0．5μg·g-1（R1组）、1．0μg·g-1（R2组）和1．5μg·g-1（R3组）每尾200μL腹腔注射免疫尼罗罗非鱼（Oreochromis niloticus），同时以Sip蛋白（s组）、LrrG蛋白（L组）以及PBS（P组）作为对照。2周后对所有免疫鱼体进行无乳链球菌（Streptococcus agalactiae）人工攻毒，攻毒剂量为其半致死浓度（LD50：4．0×10^8CFU·mL-1）。结果显示LrG—Sip重组融合蛋白R1组对尼罗罗非鱼的相对免疫保护率最高，达89．14％；且免疫后第14和第28天，该组鱼体血清抗体OD450nm值分别达0．63和0．64，均显著高于单一蛋白对照组（S和L组）和PBS组（P〈0．05）；R1组鱼体血清过氧化物酶（POD）和碱性磷酸酶（AKP）活性在上述2个时间点也显著高于其他组（P〈0．05）；但溶菌酶（LZM）和总超氧化物歧化酶（T—SOD）活性与其他组之间差异不显著（P〉0．05）。初步表明LrrG-Sip重组融合蛋白具有良好的免疫原性，其免疫原性明显优于单个蛋白，且能有效减少免疫剂量。 |
| Abstract_FL | To evaluate the immunogenicity of LrrG-Sip recombinant fusion protein,we immunized Nile tilapia (Oreochromis niloticus) by intraperitoneal (IP) injection with 200 μL LrrG-Sip recombinant fusion protein at dose of 0.5 μg·g-1 (R1 group),1.0 μg·g-1 (R2 group) and 1.5 μg·g-1 (R3 group),respectively. Meanwhile,control groups of Nile tilapia were IP injected with 200 μL Sip protein (1.0 μg·g-1,S group),200 μL LrrG protein (1.0 μg·g-1,L group) and 200 μL sterile phosphate buffered saline (PBS,P group),respectively. On 15th day,all of the immunized fish (groups of R,S,L and P) were challenged with Streptococcus agalactiae by IP injection at LD50 dose of 100 μL (containing 4.0×108 CFU·mL-1). Results show that the relative percent survival (RPS) of fish from group R1 (0.5 μg·g-1) was the highest (89.14 %). The OD450 nm values of serum antibody of fish from group R1 were 0.63 and 0.64 on 14th day and 28th day post-immunization,respectively,significantly higher than those of the other groups (S and L)(P<0.05).The activity of peroxidase and alkaline phosphatase of fish serum from this group was also significantly higher than that of the other groups at the above two time points (P<0.05). There was no significant difference in the activity of lysozyme and total superoxide dismutase of fish serum among different protein immunization groups(P>0.05). The results suggest that LrrG-Sip fusion protein has better immunogenicity than single protein LrrG or Sip,which can also reduce the injection dose effectively. |
| Author | 曾祖聪 可小丽 卢迈新 刘志刚 曹建萌 高风英 朱华平 王淼 |
| AuthorAffiliation | 中国水产科学研究院珠江水产研究所农业部热带亚热带水产资源利用与养殖重点实验室,广东广州510380 上海海洋大学水产与生命学院,上海201306 |
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| Author_FL | GAO Fengying LIU Zhigang WANG Miao ZENG Zucong KE Xiaoli LU Maixin ZHU Huaping CAO Jianmeng |
| Author_FL_xml | – sequence: 1 fullname: ZENG Zucong – sequence: 2 fullname: KE Xiaoli – sequence: 3 fullname: LU Maixin – sequence: 4 fullname: LIU Zhigang – sequence: 5 fullname: CAO Jianmeng – sequence: 6 fullname: GAO Fengying – sequence: 7 fullname: ZHU Huaping – sequence: 8 fullname: WANG Miao |
| Author_xml | – sequence: 1 fullname: 曾祖聪 可小丽 卢迈新 刘志刚 曹建萌 高风英 朱华平 王淼 |
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| DOI | 10.3969/j.issn.2095-0780.2017.03.007 |
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| DocumentTitleAlternate | Immunogenicity analysis of LrrG-Sip fusion protein of Streptococcus agalactiae in tilapia |
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| Keywords | tilapia 无乳链球菌 罗非鱼 LrrG-Sip recombinant fusion protein Streptococcus agalactiae LrrG-Sip重组融合蛋白 immunogenicity 免疫原性 |
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| Notes | ZENG Zucong1,2, KE Xiaoli1 , LU Maixin1 , LIU Zhigang1 , CAO Jianmeng 1 , GAO Fengying1 , ZHU Huaping1 , WANG Miao1 （ 1. Key Lab. of Tropical ＆ Subtropical Fishery Resource Application ＆ Cultivation, Ministry of Agriculture; Pearl River Fisheries Research Institute, Chinese Academy of Fisheries Science, Guangzhou 510380, China; 2. College of Fisheries ＆ Life Science, Shanghai Ocean University, Shanghai 201306, China） Streptococcus agalactiae; tilapia; LrrG-Sip recombinant fusion protein; immunogenicity 44-1683/S To evaluate the immunogenicity of LrrG-Sip recombinant fusion protein, we immunized Nile tilapia （Oreochromis niloticus） by intraperitoneal （IP） injection with 200 μL LrrG-Sip recombinant fusion protein at dose of 0. 5 μg ·g-1 （ R1 group）, 1.0 μg ·g-1 （R2 group） and 1.5 μg·g-1 （R3 group）, respectively. Meanwhile, control groups of Nile tilapia were IP injected with 200 μL Sip protein （ 1.0 μg·g-1, S group）, 200 μL LrrG protein （ 1.0 μg·g-1, L group） and 200 ixL sterile phosphate buffered saline （ PBS, P |
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| PublicationTitle | 南方水产科学 |
| PublicationTitleAlternate | South China Fisheries Science |
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| PublicationYear | 2017 |
| Publisher | 中国水产科学研究院珠江水产研究所,农业部热带亚热带水产资源利用与养殖重点实验室,广东 广州 510380 上海海洋大学水产与生命学院,上海 201306%中国水产科学研究院珠江水产研究所,农业部热带亚热带水产资源利用与养殖重点实验室,广东 广州 510380 |
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| Snippet | 为探究无乳链球菌LrrG（Leucine—rich repeat protein from GBS）和表面免疫原性蛋白Sip（surface immunogenic... S924.5; 为探究无乳链球菌LrrG(Leucine-rich repeat protein from GBS)和表面免疫原性蛋白Sip(surface immunogenic protein)串联表达的LrrG-Sip重组融合蛋白的免疫原性,该研究将原核表达的LrrG-Sip重组融合蛋白分别以0.5... |
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| SubjectTerms | LrrG-Sip重组融合蛋白 免疫原性 无乳链球菌 罗非鱼 |
| Title | 罗非鱼无乳链球菌LrrG-Sip融合蛋白免疫原性研究 |
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