Nucleo-cytoplasmic shuttling of splicing factor SRSF1 is required for development and cilia function

• Published in: eLife Vol. 10
• Main Authors: Haward, Fiona, Maslon, Magdalena M, Yeyati, Patricia L, Bellora, Nicolas, Hansen, Jan N, Aitken, Stuart, Lawson, Jennifer, von Kriegsheim, Alex, Wachten, Dagmar, Mill, Pleasantine, Adams, Ian R, Caceres, Javier F
• Format: Journal Article
• Language: English
• Published: England eLife Science Publications, Ltd 02.08.2021; eLife Sciences Publications Ltd; eLife Sciences Publications, Ltd
• Subjects: alternative splicing; Analysis; Animals; Binding proteins; Biochemistry and Chemical Biology; Body size; Cell cycle; Cell Nucleus - metabolism; Chromosomes and Gene Expression; Cilia; Cilia - metabolism; CRISPR; Cytoplasm; Cytoplasm - metabolism; Gene expression; Genome editing; Genomes; Genomics; Hydrocephalus; Kinases; Localization; Male; Mice; motile cilia; mRNA translation; Physiological aspects; Protein binding; Proteins; RNA; RNA polymerase; RNA-binding protein; RNA-binding proteins; Serine-Arginine Splicing Factors - genetics; Serine-Arginine Splicing Factors - metabolism; Splicing factors; SR proteins; SRSF1; Stem cells; Testes; Ultrastructure; Canada; SR proteins; mouse; mRNA translation; alternative splicing; chemical biology; SRSF1; biochemistry; chromosomes; motile cilia; RNA-binding proteins; gene expression

Shuttling RNA-binding proteins coordinate nuclear and cytoplasmic steps of gene expression. The SR family proteins regulate RNA splicing in the nucleus and a subset of them, including SRSF1, shuttles between the nucleus and cytoplasm affecting post-splicing processes. However, the physiological sign...