IFT trains in different stages of assembly queue at the ciliary base for consecutive release into the cilium

• Published in: eLife Vol. 6
• Main Authors: Wingfield, Jenna L, Mengoni, Ilaria, Bomberger, Heather, Jiang, Yu-Yang, Walsh, Jonathon D, Brown, Jason M, Picariello, Tyler, Cochran, Deborah A, Zhu, Bing, Pan, Junmin, Eggenschwiler, Jonathan, Gaertig, Jacek, Witman, George B, Kner, Peter, Lechtreck, Karl
• Format: Journal Article
• Language: English
• Published: England eLife Science Publications, Ltd 31.05.2017; eLife Sciences Publications Ltd; eLife Sciences Publications, Ltd
• Subjects: Active biological transport; Basal bodies; Biology; Cameras; Cell Biology; Cell body; Cilia; Dynein; flagella; Fluorescence recovery after photobleaching; IFT; Laboratories; Life sciences; Observations; Physiological aspects; Proteins; Tubulin; flagella; cell biology; chlamydomonas reinhardtii; IFT

Intraflagellar transport (IFT) trains, multimegadalton assemblies of IFT proteins and motors, traffic proteins in cilia. To study how trains assemble, we employed fluorescence protein-tagged IFT proteins in Chlamydomonas reinhardtii. IFT-A and motor proteins are recruited from the cell body to the b...