HOPS recognizes each SNARE, assembling ternary trans -complexes for rapid fusion upon engagement with the 4th SNARE
Yeast vacuole fusion requires R-SNARE, Q-SNAREs, and HOPS. A HOPS SM-family subunit binds the R- and Qa-SNAREs. We now report that HOPS binds each of the four SNAREs. HOPS catalyzes fusion when the Q-SNAREs are not pre-assembled, ushering them into a functional complex. Co-incubation of HOPS, proteo...
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Abstract | Yeast vacuole fusion requires R-SNARE, Q-SNAREs, and HOPS. A HOPS SM-family subunit binds the R- and Qa-SNAREs. We now report that HOPS binds each of the four SNAREs. HOPS catalyzes fusion when the Q-SNAREs are not pre-assembled, ushering them into a functional complex. Co-incubation of HOPS, proteoliposomes bearing R-SNARE, and proteoliposomes with any two Q-SNAREs yields a rapid-fusion complex with 3 SNAREs in a
-assembly. The missing Q-SNARE then induces sudden fusion. HOPS can 'template' SNARE complex assembly through SM recognition of R- and Qa-SNAREs. Though the Qa-SNARE is essential for spontaneous SNARE assembly, HOPS also assembles a rapid-fusion complex between R- and QbQc-SNARE proteoliposomes in the absence of Qa-SNARE, awaiting Qa for fusion. HOPS-dependent fusion is saturable at low concentrations of each Q-SNARE, showing binding site functionality. HOPS thus tethers membranes and recognizes each SNARE, assembling R+Qa or R+QbQc rapid fusion intermediates. |
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AbstractList | Yeast vacuole fusion requires R-SNARE, Q-SNAREs, and HOPS. A HOPS SM-family subunit binds the R- and Qa-SNAREs. We now report that HOPS binds each of the four SNAREs. HOPS catalyzes fusion when the Q-SNAREs are not pre-assembled, ushering them into a functional complex. Co-incubation of HOPS, proteoliposomes bearing R-SNARE, and proteoliposomes with any two Q-SNAREs yields a rapid-fusion complex with 3 SNAREs in a
trans
-assembly. The missing Q-SNARE then induces sudden fusion. HOPS can ‘template’ SNARE complex assembly through SM recognition of R- and Qa-SNAREs. Though the Qa-SNARE is essential for spontaneous SNARE assembly, HOPS also assembles a rapid-fusion complex between R- and QbQc-SNARE proteoliposomes in the absence of Qa-SNARE, awaiting Qa for fusion. HOPS-dependent fusion is saturable at low concentrations of each Q-SNARE, showing binding site functionality. HOPS thus tethers membranes and recognizes each SNARE, assembling R+Qa or R+QbQc rapid fusion intermediates. Yeast vacuole fusion requires R-SNARE, Q-SNAREs, and HOPS. A HOPS SM-family subunit binds the R- and Qa-SNAREs. We now report that HOPS binds each of the four SNAREs. HOPS catalyzes fusion when the Q-SNAREs are not pre-assembled, ushering them into a functional complex. Co-incubation of HOPS, proteoliposomes bearing R-SNARE, and proteoliposomes with any two Q-SNAREs yields a rapid-fusion complex with 3 SNAREs in a trans-assembly. The missing Q-SNARE then induces sudden fusion. HOPS can ‘template’ SNARE complex assembly through SM recognition of R- and Qa-SNAREs. Though the Qa-SNARE is essential for spontaneous SNARE assembly, HOPS also assembles a rapid-fusion complex between R- and QbQc-SNARE proteoliposomes in the absence of Qa-SNARE, awaiting Qa for fusion. HOPS-dependent fusion is saturable at low concentrations of each Q-SNARE, showing binding site functionality. HOPS thus tethers membranes and recognizes each SNARE, assembling R+Qa or R+QbQc rapid fusion intermediates. Yeast vacuole fusion requires R-SNARE, Q-SNAREs, and HOPS. A HOPS SM-family subunit binds the R- and Qa-SNAREs. We now report that HOPS binds each of the four SNAREs. HOPS catalyzes fusion when the Q-SNAREs are not pre-assembled, ushering them into a functional complex. Co-incubation of HOPS, proteoliposomes bearing R-SNARE, and proteoliposomes with any two Q-SNAREs yields a rapid-fusion complex with 3 SNAREs in a -assembly. The missing Q-SNARE then induces sudden fusion. HOPS can 'template' SNARE complex assembly through SM recognition of R- and Qa-SNAREs. Though the Qa-SNARE is essential for spontaneous SNARE assembly, HOPS also assembles a rapid-fusion complex between R- and QbQc-SNARE proteoliposomes in the absence of Qa-SNARE, awaiting Qa for fusion. HOPS-dependent fusion is saturable at low concentrations of each Q-SNARE, showing binding site functionality. HOPS thus tethers membranes and recognizes each SNARE, assembling R+Qa or R+QbQc rapid fusion intermediates. |
Audience | Academic |
Author | Orr, Amy S Wickner, William T Lee, Miriam Song, Hongki Harner, Max E |
Author_xml | – sequence: 1 givenname: Hongki orcidid: 0000-0002-3761-5434 surname: Song fullname: Song, Hongki organization: Department of Biochemistry and Cell Biology, Geisel School of Medicine at Dartmouth, Hanover, United States – sequence: 2 givenname: Amy S surname: Orr fullname: Orr, Amy S organization: Department of Biochemistry and Cell Biology, Geisel School of Medicine at Dartmouth, Hanover, United States – sequence: 3 givenname: Miriam surname: Lee fullname: Lee, Miriam organization: Department of Biochemistry and Cell Biology, Geisel School of Medicine at Dartmouth, Hanover, United States – sequence: 4 givenname: Max E orcidid: 0000-0002-5513-1046 surname: Harner fullname: Harner, Max E organization: Department of Biochemistry and Cell Biology, Geisel School of Medicine at Dartmouth, Hanover, United States – sequence: 5 givenname: William T orcidid: 0000-0001-8431-0468 surname: Wickner fullname: Wickner, William T organization: Department of Biochemistry and Cell Biology, Geisel School of Medicine at Dartmouth, Hanover, United States |
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Keywords | yeast vacuoles chemical biology biochemistry HOPS S. cerevisiae SNARE membrane fusion |
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Snippet | Yeast vacuole fusion requires R-SNARE, Q-SNAREs, and HOPS. A HOPS SM-family subunit binds the R- and Qa-SNAREs. We now report that HOPS binds each of the four... |
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SubjectTerms | Binding sites Biochemistry and Chemical Biology HOPS Intermediates Intracellular Membranes - metabolism Lipids Membrane Fusion Membranes Protein Binding Proteins Questions and answers R-SNARE Proteins - metabolism Saccharomyces cerevisiae - metabolism Saccharomyces cerevisiae Proteins - metabolism SNAP receptors SNARE Vacuoles - metabolism yeast vacuoles |
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Title | HOPS recognizes each SNARE, assembling ternary trans -complexes for rapid fusion upon engagement with the 4th SNARE |
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