Ataxin-7 and Non-stop coordinate SCAR protein levels, subcellular localization, and actin cytoskeleton organization

Atxn7, a subunit of SAGA chromatin remodeling complex, is subject to polyglutamine expansion at the amino terminus, causing spinocerebellar ataxia type 7 (SCA7), a progressive retinal and neurodegenerative disease. Within SAGA, the Atxn7 amino terminus anchors Non-stop, a deubiquitinase, to the comp...

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Published ineLife Vol. 8
Main Authors Cloud, Veronica, Thapa, Ada, Morales-Sosa, Pedro, Miller, Tayla M, Miller, Sara A, Holsapple, Daniel, Gerhart, Paige M, Momtahan, Elaheh, Jack, Jarrid L, Leiva, Edgardo, Rapp, Sarah R, Shelton, Lauren G, Pierce, Richard A, Martin-Brown, Skylar, Florens, Laurence, Washburn, Michael P, Mohan, Ryan D
Format Journal Article
LanguageEnglish
Published England eLife Science Publications, Ltd 26.07.2019
eLife Sciences Publications Ltd
eLife Sciences Publications, Ltd
Subjects
Actin
Actin Cytoskeleton - metabolism
Animals
Ataxia
Ataxin
Ataxin-7 - metabolism
Ataxin7
Cell Biology
Chromatin
Chromatin remodeling
Cytoskeleton
Disease
Drosophila melanogaster - physiology
Drosophila Proteins - metabolism
Endopeptidases - metabolism
Enzymes
Gene expression
Gene Expression Regulation
Genotype & phenotype
Haploinsufficiency
Insects
Localization
Mass spectrometry
Microfilament Proteins - metabolism
Mutation
Nervous system diseases
neurobiology
Neurodegeneration
Neurodegenerative diseases
Neuroscience
Non-stop
Peptides
Physiological aspects
Polyglutamine
Proteasomes
Protein Interaction Mapping
Protein Interaction Maps
Proteins
Retina
SAGA complex
Scientific imaging
Spinocerebellar ataxia
Spinocerebellar ataxias
Trinucleotide repeat diseases
WAVE regulatory complex
France
United States > US
Kansas City Missouri
actin
cell biology
Non-stop
neuroscience
WAVE regulatory complex
neurobiology
D. melanogaster
SAGA complex
Ataxin7
Online AccessGet full text
ISSN2050-084X
2050-084X
DOI10.7554/eLife.49677

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Abstract Atxn7, a subunit of SAGA chromatin remodeling complex, is subject to polyglutamine expansion at the amino terminus, causing spinocerebellar ataxia type 7 (SCA7), a progressive retinal and neurodegenerative disease. Within SAGA, the Atxn7 amino terminus anchors Non-stop, a deubiquitinase, to the complex. To understand the scope of Atxn7-dependent regulation of Non-stop, substrates of the deubiquitinase were sought. This revealed Non-stop, dissociated from Atxn7, interacts with Arp2/3 and WAVE regulatory complexes (WRC), which control actin cytoskeleton assembly. There, Non-stop countered polyubiquitination and proteasomal degradation of WRC subunit SCAR. Dependent on conserved WRC interacting receptor sequences (WIRS), Non-stop augmentation increased protein levels, and directed subcellular localization, of SCAR, decreasing cell area and number of protrusions. In vivo, heterozygous mutation of SCAR did not significantly rescue knockdown of Atxn7, but heterozygous mutation of Atxn7 rescued haploinsufficiency of SCAR.
AbstractList Atxn7, a subunit of SAGA chromatin remodeling complex, is subject to polyglutamine expansion at the amino terminus, causing spinocerebellar ataxia type 7 (SCA7), a progressive retinal and neurodegenerative disease. Within SAGA, the Atxn7 amino terminus anchors Non-stop, a deubiquitinase, to the complex. To understand the scope of Atxn7-dependent regulation of Non-stop, substrates of the deubiquitinase were sought. This revealed Non-stop, dissociated from Atxn7, interacts with Arp2/3 and WAVE regulatory complexes (WRC), which control actin cytoskeleton assembly. There, Non-stop countered polyubiquitination and proteasomal degradation of WRC subunit SCAR. Dependent on conserved WRC interacting receptor sequences (WIRS), Non-stop augmentation increased protein levels, and directed subcellular localization, of SCAR, decreasing cell area and number of protrusions. In vivo, heterozygous mutation of SCAR did not significantly rescue knockdown of Atxn7, but heterozygous mutation of Atxn7 rescued haploinsufficiency of SCAR.
Atxn7, a subunit of SAGA chromatin remodeling complex, is subject to polyglutamine expansion at the amino terminus, causing spinocerebellar ataxia type 7 (SCA7), a progressive retinal and neurodegenerative disease. Within SAGA, the Atxn7 amino terminus anchors Non-stop, a deubiquitinase, to the complex. To understand the scope of Atxn7-dependent regulation of Non-stop, substrates of the deubiquitinase were sought. This revealed Non-stop, dissociated from Atxn7, interacts with Arp2/3 and WAVE regulatory complexes (WRC), which control actin cytoskeleton assembly. There, Non-stop countered polyubiquitination and proteasomal degradation of WRC subunit SCAR. Dependent on conserved RC nteracting eceptor equences (WIRS), Non-stop augmentation increased protein levels, and directed subcellular localization, of SCAR, decreasing cell area and number of protrusions. , heterozygous mutation of SCAR did not significantly rescue knockdown of Atxn7, but heterozygous mutation of Atxn7 rescued haploinsufficiency of SCAR.
Atxn7, a subunit of SAGA chromatin remodeling complex, is subject to polyglutamine expansion at the amino terminus, causing spinocerebellar ataxia type 7 (SCA7), a progressive retinal and neurodegenerative disease. Within SAGA, the Atxn7 amino terminus anchors Non-stop, a deubiquitinase, to the complex. To understand the scope of Atxn7-dependent regulation of Non-stop, substrates of the deubiquitinase were sought. This revealed Non-stop, dissociated from Atxn7, interacts with Arp2/3 and WAVE regulatory complexes (WRC), which control actin cytoskeleton assembly. There, Non-stop countered polyubiquitination and proteasomal degradation of WRC subunit SCAR. Dependent on conserved W RC i nteracting r eceptor s equences (WIRS), Non-stop augmentation increased protein levels, and directed subcellular localization, of SCAR, decreasing cell area and number of protrusions. In vivo , heterozygous mutation of SCAR did not significantly rescue knockdown of Atxn7, but heterozygous mutation of Atxn7 rescued haploinsufficiency of SCAR.
Atxn7, a subunit of SAGA chromatin remodeling complex, is subject to polyglutamine expansion at the amino terminus, causing spinocerebellar ataxia type 7 (SCA7), a progressive retinal and neurodegenerative disease. Within SAGA, the Atxn7 amino terminus anchors Non-stop, a deubiquitinase, to the complex. To understand the scope of Atxn7-dependent regulation of Non-stop, substrates of the deubiquitinase were sought. This revealed Non-stop, dissociated from Atxn7, interacts with Arp2/3 and WAVE regulatory complexes (WRC), which control actin cytoskeleton assembly. There, Non-stop countered polyubiquitination and proteasomal degradation of WRC subunit SCAR. Dependent on conserved WRC interacting receptor sequences (WIRS), Non-stop augmentation increased protein levels, and directed subcellular localization, of SCAR, decreasing cell area and number of protrusions. In vivo, heterozygous mutation of SCAR did not significantly rescue knockdown of Atxn7, but heterozygous mutation of Atxn7 rescued haploinsufficiency of SCAR.Atxn7, a subunit of SAGA chromatin remodeling complex, is subject to polyglutamine expansion at the amino terminus, causing spinocerebellar ataxia type 7 (SCA7), a progressive retinal and neurodegenerative disease. Within SAGA, the Atxn7 amino terminus anchors Non-stop, a deubiquitinase, to the complex. To understand the scope of Atxn7-dependent regulation of Non-stop, substrates of the deubiquitinase were sought. This revealed Non-stop, dissociated from Atxn7, interacts with Arp2/3 and WAVE regulatory complexes (WRC), which control actin cytoskeleton assembly. There, Non-stop countered polyubiquitination and proteasomal degradation of WRC subunit SCAR. Dependent on conserved WRC interacting receptor sequences (WIRS), Non-stop augmentation increased protein levels, and directed subcellular localization, of SCAR, decreasing cell area and number of protrusions. In vivo, heterozygous mutation of SCAR did not significantly rescue knockdown of Atxn7, but heterozygous mutation of Atxn7 rescued haploinsufficiency of SCAR.
Audience Academic
Author Martin-Brown, Skylar
Miller, Tayla M
Rapp, Sarah R
Miller, Sara A
Washburn, Michael P
Morales-Sosa, Pedro
Gerhart, Paige M
Leiva, Edgardo
Shelton, Lauren G
Pierce, Richard A
Mohan, Ryan D
Holsapple, Daniel
Florens, Laurence
Cloud, Veronica
Jack, Jarrid L
Thapa, Ada
Momtahan, Elaheh
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BackLink https://www.ncbi.nlm.nih.gov/pubmed/31348003$$D View this record in MEDLINE/PubMed
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Cites_doi 10.1242/jcs.114.10.1801
10.1007/s12035-012-8391-5
10.1152/physrev.00018.2013
10.1016/j.devcel.2004.11.020
10.1007/s00018-016-2255-5
10.1371/journal.pbio.1001238
10.1016/j.celrep.2014.06.037
10.1371/journal.pone.0043112
10.1038/ncomms6511
10.1016/j.cell.2010.07.045
10.1128/MCB.05231-11
10.1080/23262133.2016.1261653
10.1128/MCB.01454-14
10.1523/JNEUROSCI.5453-06.2007
10.3389/fncel.2015.00327
10.1016/j.molcel.2005.02.013
10.1074/jbc.M607596200
10.2741/1325
10.1016/B978-0-444-51892-7.00030-9
10.1101/gr.112961.110
10.1083/jcb.200303023
10.1126/scitranslmed.3000813
10.1038/sj.emboj.7601966
10.1534/genetics.115.180208
10.1038/ng0997-65
10.1016/j.molcel.2007.12.011
10.1016/j.molcel.2009.06.015
10.1016/j.cub.2003.10.005
10.1186/gb-2009-10-3-r26
10.1086/302406
10.1016/S0959-440X(02)00396-2
10.1074/jbc.M114.631663
10.1172/JCI23412
10.1021/ac9023999
10.1128/MCB.23.9.3305-3319.2003
10.1371/journal.pone.0107895
10.1002/pmic.201500344
10.1038/s41467-018-03455-x
10.1016/j.cell.2013.11.048
10.1016/j.cub.2015.01.052
10.1016/j.ydbio.2004.07.043
10.1126/science.1190049
10.1038/embor.2011.140
10.1016/j.gde.2014.06.008
10.1073/pnas.88.9.3832
10.15252/embr.201745680
10.1038/nsmb.1397
10.1038/85686
10.1093/nar/16.3.1043
10.1128/MCB.01121-09
10.1242/jcs.199570
10.1038/nature05954
10.1016/B978-0-12-336156-1.50061-6
10.3389/fnmol.2014.00072
10.1016/j.molcel.2015.07.033
10.1038/ncomms10098
10.1007/BF02632042
10.1016/j.cell.2010.04.026
10.1016/j.ab.2010.02.036
10.1523/JNEUROSCI.4000-11.2011
10.1101/gad.1144003
10.1186/gb-2009-10-6-226
10.1002/dvdy.23742
10.1074/jbc.M114.624684
10.1083/jcb.200109057
10.1046/j.1471-4159.2002.01185.x
10.1038/ncomms3641
10.1126/science.1240376
10.1101/gad.1898410
10.1038/msb.2011.40
10.1016/j.devcel.2006.05.004
10.2174/1567202612666150603141144
10.1016/j.molcel.2013.04.012
10.1186/1756-8935-2-2
10.1038/nmeth.2019
10.1016/j.ejcb.2010.08.011
10.1016/S0896-6273(01)00183-0
10.1016/S0960-9822(98)00015-3
10.1007/978-1-61779-043-0_17
10.1080/07853890410031948
10.1101/gad.225151.113
10.1016/j.molcel.2005.04.024
10.1101/gad.250225.114
10.1074/jbc.M705265200
10.4161/21541248.2014.982415
10.1038/nature09623
10.1038/ncb1733
10.1073/pnas.0509927103
10.1083/jcb.105.4.1473
10.1016/j.devcel.2014.12.019
10.1126/science.1103455
10.3791/4270
10.1128/MCB.26.3.871-882.2006
10.1534/g3.116.031310
10.1016/0896-6273(95)90281-3
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2019, Cloud et al. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.
2019, Cloud et al 2019 Cloud et al
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Keywords actin
cell biology
Non-stop
neuroscience
WAVE regulatory complex
neurobiology
D. melanogaster
SAGA complex
Ataxin7
Language English
License http://creativecommons.org/licenses/by/4.0
2019, Cloud et al.
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References Setiaputra (bib71) 2015; 290
Poeck (bib60) 2001; 29
Giunti (bib20) 1999; 64
Rogers (bib67) 2003; 162
Xia (bib87) 2012; 10
Navarro-Lérida (bib57) 2015; 32
Machesky (bib46) 1998; 8
Taylor (bib75) 2010; 2
Garden (bib19) 1993
Köhler (bib30) 2010; 141
Verboon (bib81) 2015; 25
Ma (bib45) 2016; 6
Tsou (bib77) 2012; 7
Martin (bib47) 1995; 14
Meulmeester (bib50) 2005; 18
Kusch (bib34) 2003; 23
Lee (bib39) 2009; 2
Lee (bib41) 2016; 16
Mohan (bib54) 2014; 28
Miyamoto (bib52) 2013; 341
Romero-Calvo (bib68) 2010; 401
Ristic (bib65) 2014; 7
Chou (bib13) 2016; 3
Liu (bib44) 2009; 10
Bray (bib6) 2005; 8
Li (bib42) 2018; 9
Cooper (bib14) 1987; 105
Rabinovich (bib63) 2015; 9
Dumpich (bib17) 2015; 12
Takenawa (bib74) 2001; 114
Zhang (bib94) 2010; 82
Kessels (bib28) 2011; 90
Young (bib90) 2007; 282
Wang (bib84) 2006; 103
Samara (bib69) 2010; 328
Glinsky (bib21) 2005; 115
Blanchoin (bib4) 2014; 94
Ui (bib78) 1994; 4
Prithviraj (bib62) 2012; 65
Atanassov (bib3) 2011; 12
Lan (bib36) 2015; 35
Zallen (bib92) 2002; 156
Verboon (bib82) 2015; 6
Yu (bib91) 2011; 723
Chen (bib11) 2014; 156
Chen (bib10) 2010; 468
Tran (bib76) 2015; 6
Mohan (bib53) 2010; 24
Alekhina (bib1) 2017; 130
Campello (bib8) 2013; 47
Kurisu (bib33) 2009; 10
Cetera (bib9) 2014; 5
Cherbas (bib12) 2011; 21
Koken (bib31) 1991; 88
Perkins (bib58) 2015; 201
Köhler (bib29) 2008; 10
Lee (bib40) 2011; 7
Furrer (bib18) 2011; 31
Zhao (bib95) 2008; 29
Bunch (bib7) 1988; 16
David (bib15) 1997; 17
Yang (bib88) 2015; 290
Lang (bib37) 2011; 31
Zuiderveld (bib96) 1994; 85
Meyer (bib51) 2002; 83
Guelman (bib23) 2006; 26
Martin (bib48) 2012; 103
Zhang (bib93) 2006; 10
Weake (bib86) 2008; 27
Suganuma (bib73) 2010; 142
Grice (bib22) 2016; 73
Kusch (bib35) 2004; 306
Washburn (bib85) 2001; 19
Yoo (bib89) 2007; 282
Atanassov (bib2) 2009; 35
Bonnet (bib5) 2014; 28
Kunda (bib32) 2003; 13
Morrow (bib56) 2018; 19
Pollard (bib61) 2002; 12
Rawe (bib64) 2004; 276
Suganuma (bib72) 2008; 15
Lim (bib43) 2013; 4
Henry (bib26) 2003; 17
Rodriguez-Mesa (bib66) 2012; 241
Vishavkarma (bib83) 2014; 9
van der Knaap (bib80) 2010; 30
Dietzl (bib16) 2007; 448
Latouche (bib38) 2007; 27
Mohan (bib55) 2014; 26
Haddad (bib24) 2013; 50
Petrucelli (bib59) 2004; 36
Schindelin (bib70) 2012; 9
Hao (bib25) 2015; 59
Irie (bib27) 2004; 9
van der Knaap (bib79) 2005; 17
McCormick (bib49) 2014; 8
References_xml – volume: 114
  start-page: 1801
  year: 2001
  ident: bib74
  article-title: WASP and WAVE family proteins: key molecules for rapid rearrangement of cortical actin filaments and cell movement
  publication-title: Journal of Cell Science
  doi: 10.1242/jcs.114.10.1801
– volume: 47
  start-page: 790
  year: 2013
  ident: bib8
  article-title: The ubiquitin-proteasome system in retinal health and disease
  publication-title: Molecular Neurobiology
  doi: 10.1007/s12035-012-8391-5
– volume: 94
  start-page: 235
  year: 2014
  ident: bib4
  article-title: Actin dynamics, architecture, and mechanics in cell motility
  publication-title: Physiological Reviews
  doi: 10.1152/physrev.00018.2013
– volume: 8
  start-page: 279
  year: 2005
  ident: bib6
  article-title: Bre1 is required for notch signaling and histone modification
  publication-title: Developmental Cell
  doi: 10.1016/j.devcel.2004.11.020
– volume: 73
  start-page: 3497
  year: 2016
  ident: bib22
  article-title: The recognition of ubiquitinated proteins by the proteasome
  publication-title: Cellular and Molecular Life Sciences
  doi: 10.1007/s00018-016-2255-5
– volume: 10
  year: 2012
  ident: bib87
  article-title: USP8 promotes smoothened signaling by preventing its ubiquitination and changing its subcellular localization
  publication-title: PLOS Biology
  doi: 10.1371/journal.pbio.1001238
– volume: 8
  start-page: 477
  year: 2014
  ident: bib49
  article-title: The SAGA histone deubiquitinase module controls yeast replicative lifespan via Sir2 interaction
  publication-title: Cell Reports
  doi: 10.1016/j.celrep.2014.06.037
– volume: 7
  year: 2012
  ident: bib77
  article-title: Systematic analysis of the physiological importance of deubiquitinating enzymes
  publication-title: PLOS ONE
  doi: 10.1371/journal.pone.0043112
– volume: 5
  year: 2014
  ident: bib9
  article-title: Epithelial rotation promotes the global alignment of contractile actin bundles during Drosophila egg chamber elongation
  publication-title: Nature Communications
  doi: 10.1038/ncomms6511
– volume: 142
  start-page: 726
  year: 2010
  ident: bib73
  article-title: The ATAC acetyltransferase complex coordinates MAP kinases to regulate JNK target genes
  publication-title: Cell
  doi: 10.1016/j.cell.2010.07.045
– volume: 31
  start-page: 3734
  year: 2011
  ident: bib37
  article-title: The tightly controlled deubiquitination activity of the human SAGA complex differentially modifies distinct gene regulatory elements
  publication-title: Molecular and Cellular Biology
  doi: 10.1128/MCB.05231-11
– volume: 3
  year: 2016
  ident: bib13
  article-title: The Arp2/3 complex is essential at multiple stages of neural development
  publication-title: Neurogenesis
  doi: 10.1080/23262133.2016.1261653
– volume: 35
  start-page: 1777
  year: 2015
  ident: bib36
  article-title: Poly(Q) Expansions in ATXN7 affect solubility but not activity of the SAGA deubiquitinating module
  publication-title: Molecular and Cellular Biology
  doi: 10.1128/MCB.01454-14
– volume: 27
  start-page: 2483
  year: 2007
  ident: bib38
  article-title: A conditional pan-neuronal Drosophila model of spinocerebellar ataxia 7 with a reversible adult phenotype suitable for identifying modifier genes
  publication-title: Journal of Neuroscience
  doi: 10.1523/JNEUROSCI.5453-06.2007
– volume: 9
  year: 2015
  ident: bib63
  article-title: Long term ex vivo culturing of Drosophila brain as a method to live image pupal brains: insights into the cellular mechanisms of neuronal remodeling
  publication-title: Frontiers in Cellular Neuroscience
  doi: 10.3389/fncel.2015.00327
– volume: 17
  start-page: 695
  year: 2005
  ident: bib79
  article-title: GMP synthetase stimulates histone H2B deubiquitylation by the epigenetic silencer USP7
  publication-title: Molecular Cell
  doi: 10.1016/j.molcel.2005.02.013
– volume: 282
  start-page: 7616
  year: 2007
  ident: bib89
  article-title: A novel role of the actin-nucleating Arp2/3 complex in the regulation of RNA polymerase II-dependent transcription
  publication-title: Journal of Biological Chemistry
  doi: 10.1074/jbc.M607596200
– volume: 9
  year: 2004
  ident: bib27
  article-title: EPHB receptor signaling in dendritic spine development
  publication-title: Frontiers in Bioscience
  doi: 10.2741/1325
– volume: 103
  start-page: 475
  year: 2012
  ident: bib48
  article-title: Spinocerebellar ataxia type 7
  publication-title: Handbook of Clinical Neurology
  doi: 10.1016/B978-0-444-51892-7.00030-9
– volume: 21
  start-page: 301
  year: 2011
  ident: bib12
  article-title: The transcriptional diversity of 25 Drosophila cell lines
  publication-title: Genome Research
  doi: 10.1101/gr.112961.110
– volume: 162
  start-page: 1079
  year: 2003
  ident: bib67
  article-title: Molecular requirements for actin-based Lamella formation in Drosophila S2 cells
  publication-title: The Journal of Cell Biology
  doi: 10.1083/jcb.200303023
– volume: 2
  year: 2010
  ident: bib75
  article-title: Nuclear role of WASp in the pathogenesis of dysregulated TH1 immunity in human Wiskott-Aldrich syndrome
  publication-title: Science Translational Medicine
  doi: 10.1126/scitranslmed.3000813
– volume: 27
  start-page: 394
  year: 2008
  ident: bib86
  article-title: SAGA-mediated H2B deubiquitination controls the development of neuronal connectivity in the Drosophila visual system
  publication-title: The EMBO Journal
  doi: 10.1038/sj.emboj.7601966
– volume: 201
  start-page: 843
  year: 2015
  ident: bib58
  article-title: The transgenic RNAi project at Harvard medical school: resources and validation
  publication-title: Genetics
  doi: 10.1534/genetics.115.180208
– volume: 17
  start-page: 65
  year: 1997
  ident: bib15
  article-title: Cloning of the SCA7 gene reveals a highly unstable CAG repeat expansion
  publication-title: Nature Genetics
  doi: 10.1038/ng0997-65
– volume-title: GeneReviews
  year: 1993
  ident: bib19
– volume: 29
  start-page: 92
  year: 2008
  ident: bib95
  article-title: A TFTC/STAGA module mediates histone H2A and H2B deubiquitination, coactivates nuclear receptors, and counteracts heterochromatin silencing
  publication-title: Molecular Cell
  doi: 10.1016/j.molcel.2007.12.011
– volume: 35
  start-page: 352
  year: 2009
  ident: bib2
  article-title: Gcn5 and SAGA regulate shelterin protein turnover and telomere maintenance
  publication-title: Molecular Cell
  doi: 10.1016/j.molcel.2009.06.015
– volume: 13
  start-page: 1867
  year: 2003
  ident: bib32
  article-title: Abi, Sra1, and Kette control the stability and localization of SCAR/WAVE to regulate the formation of actin-based protrusions
  publication-title: Current Biology
  doi: 10.1016/j.cub.2003.10.005
– volume: 10
  year: 2009
  ident: bib44
  article-title: Parallel RNAi screens across different cell lines identify generic and cell type-specific regulators of actin organization and cell morphology
  publication-title: Genome Biology
  doi: 10.1186/gb-2009-10-3-r26
– volume: 64
  start-page: 1594
  year: 1999
  ident: bib20
  article-title: Molecular and clinical study of 18 families with ADCA type II: evidence for genetic heterogeneity and de novo mutation
  publication-title: The American Journal of Human Genetics
  doi: 10.1086/302406
– volume: 12
  start-page: 768
  year: 2002
  ident: bib61
  article-title: Structure and function of the Arp2/3 complex
  publication-title: Current Opinion in Structural Biology
  doi: 10.1016/S0959-440X(02)00396-2
– volume: 290
  start-page: 21996
  year: 2015
  ident: bib88
  article-title: Aggregation of Polyglutamine-expanded ataxin 7 protein specifically sequesters Ubiquitin-specific protease 22 and deteriorates its deubiquitinating function in the Spt-Ada-Gcn5-Acetyltransferase (SAGA) Complex
  publication-title: Journal of Biological Chemistry
  doi: 10.1074/jbc.M114.631663
– volume: 115
  start-page: 1503
  year: 2005
  ident: bib21
  article-title: Microarray analysis identifies a death-from-cancer signature predicting therapy failure in patients with multiple types of Cancer
  publication-title: Journal of Clinical Investigation
  doi: 10.1172/JCI23412
– volume: 82
  start-page: 2272
  year: 2010
  ident: bib94
  article-title: Refinements to label free proteome quantitation: how to deal with peptides shared by multiple proteins
  publication-title: Analytical Chemistry
  doi: 10.1021/ac9023999
– volume: 23
  start-page: 3305
  year: 2003
  ident: bib34
  article-title: Two Drosophila Ada2 homologues function in different multiprotein complexes
  publication-title: Molecular and Cellular Biology
  doi: 10.1128/MCB.23.9.3305-3319.2003
– volume: 9
  year: 2014
  ident: bib83
  article-title: Role of actin filaments in correlating nuclear shape and cell spreading
  publication-title: PLOS ONE
  doi: 10.1371/journal.pone.0107895
– volume: 16
  start-page: 1863
  year: 2016
  ident: bib41
  article-title: State-of-the-art housekeeping proteins for quantitative western blotting: revisiting the first draft of the human proteome
  publication-title: Proteomics
  doi: 10.1002/pmic.201500344
– volume: 9
  year: 2018
  ident: bib42
  article-title: The H2B deubiquitinase Usp22 promotes antibody class switch recombination by facilitating non-homologous end joining
  publication-title: Nature Communications
  doi: 10.1038/s41467-018-03455-x
– volume: 156
  start-page: 195
  year: 2014
  ident: bib11
  article-title: The WAVE regulatory complex links diverse receptors to the actin cytoskeleton
  publication-title: Cell
  doi: 10.1016/j.cell.2013.11.048
– volume: 25
  start-page: 804
  year: 2015
  ident: bib81
  article-title: Wash interacts with Lamin and affects global nuclear organization
  publication-title: Current Biology
  doi: 10.1016/j.cub.2015.01.052
– volume: 276
  start-page: 253
  year: 2004
  ident: bib64
  article-title: WAVE1 intranuclear trafficking is essential for genomic and cytoskeletal dynamics during fertilization: cell-cycle-dependent shuttling between M-phase and interphase nuclei
  publication-title: Developmental Biology
  doi: 10.1016/j.ydbio.2004.07.043
– volume: 328
  start-page: 1025
  year: 2010
  ident: bib69
  article-title: Structural insights into the assembly and function of the SAGA deubiquitinating module
  publication-title: Science
  doi: 10.1126/science.1190049
– volume: 12
  start-page: 924
  year: 2011
  ident: bib3
  article-title: USP22 regulates cell proliferation by deubiquitinating the transcriptional regulator FBP1
  publication-title: EMBO Reports
  doi: 10.1038/embor.2011.140
– volume: 26
  start-page: 96
  year: 2014
  ident: bib55
  article-title: The expanding role for chromatin and transcription in polyglutamine disease
  publication-title: Current Opinion in Genetics & Development
  doi: 10.1016/j.gde.2014.06.008
– volume: 88
  start-page: 3832
  year: 1991
  ident: bib31
  article-title: Dhr6, a Drosophila homolog of the yeast DNA-repair gene RAD6
  publication-title: PNAS
  doi: 10.1073/pnas.88.9.3832
– volume: 19
  start-page: e45680
  year: 2018
  ident: bib56
  article-title: Active site alanine mutations convert deubiquitinases into high-affinity ubiquitin-binding proteins
  publication-title: EMBO Reports
  doi: 10.15252/embr.201745680
– volume: 15
  start-page: 364
  year: 2008
  ident: bib72
  article-title: ATAC is a double histone acetyltransferase complex that stimulates nucleosome sliding
  publication-title: Nature Structural & Molecular Biology
  doi: 10.1038/nsmb.1397
– volume: 19
  start-page: 242
  year: 2001
  ident: bib85
  article-title: Large-scale analysis of the yeast proteome by multidimensional protein identification technology
  publication-title: Nature Biotechnology
  doi: 10.1038/85686
– volume: 16
  start-page: 1043
  year: 1988
  ident: bib7
  article-title: Characterization and use of the Drosophila metallothionein promoter in cultured Drosophila melanogaster cells
  publication-title: Nucleic Acids Research
  doi: 10.1093/nar/16.3.1043
– volume: 30
  start-page: 736
  year: 2010
  ident: bib80
  article-title: Biosynthetic enzyme GMP synthetase cooperates with Ubiquitin-Specific protease 7 in transcriptional regulation of ecdysteroid target genes
  publication-title: Molecular and Cellular Biology
  doi: 10.1128/MCB.01121-09
– volume: 130
  start-page: 2235
  year: 2017
  ident: bib1
  article-title: Cellular functions of WASP family proteins at a glance
  publication-title: Journal of Cell Science
  doi: 10.1242/jcs.199570
– volume: 448
  start-page: 151
  year: 2007
  ident: bib16
  article-title: A genome-wide transgenic RNAi library for conditional gene inactivation in Drosophila
  publication-title: Nature
  doi: 10.1038/nature05954
– volume: 85
  start-page: 474
  year: 1994
  ident: bib96
  article-title: Contrast limited adaptive histogram equalization
  publication-title: Graphics Gems IV
  doi: 10.1016/B978-0-12-336156-1.50061-6
– volume: 7
  year: 2014
  ident: bib65
  article-title: An optimal ubiquitin-proteasome pathway in the nervous system: the role of deubiquitinating enzymes
  publication-title: Frontiers in Molecular Neuroscience
  doi: 10.3389/fnmol.2014.00072
– volume: 59
  start-page: 956
  year: 2015
  ident: bib25
  article-title: USP7 acts as a molecular rheostat to promote WASH-Dependent endosomal protein recycling and is mutated in a human neurodevelopmental disorder
  publication-title: Molecular Cell
  doi: 10.1016/j.molcel.2015.07.033
– volume: 6
  year: 2015
  ident: bib76
  article-title: Arp2/3-mediated F-actin formation controls regulated exocytosis in vivo
  publication-title: Nature Communications
  doi: 10.1038/ncomms10098
– volume: 4
  start-page: 209
  year: 1994
  ident: bib78
  article-title: Newly established cell lines from Drosophila larval CNS express neural specific characteristics
  publication-title: In Vitro Cellular & Developmental Biology Animal
  doi: 10.1007/BF02632042
– volume: 141
  start-page: 606
  year: 2010
  ident: bib30
  article-title: Structural basis for assembly and activation of the heterotetrameric SAGA histone H2B deubiquitinase module
  publication-title: Cell
  doi: 10.1016/j.cell.2010.04.026
– volume: 401
  start-page: 318
  year: 2010
  ident: bib68
  article-title: Reversible ponceau staining as a loading control alternative to actin in western blots
  publication-title: Analytical Biochemistry
  doi: 10.1016/j.ab.2010.02.036
– volume: 31
  start-page: 16269
  year: 2011
  ident: bib18
  article-title: Spinocerebellar ataxia type 7 cerebellar disease requires the coordinated action of mutant ataxin-7 in neurons and Glia, and displays non-cell-autonomous bergmann Glia degeneration
  publication-title: Journal of Neuroscience
  doi: 10.1523/JNEUROSCI.4000-11.2011
– volume: 17
  start-page: 2648
  year: 2003
  ident: bib26
  article-title: Transcriptional activation via sequential histone H2B ubiquitylation and deubiquitylation, mediated by SAGA-associated Ubp8
  publication-title: Genes & Development
  doi: 10.1101/gad.1144003
– volume: 10
  year: 2009
  ident: bib33
  article-title: The WASP and WAVE family proteins
  publication-title: Genome Biology
  doi: 10.1186/gb-2009-10-6-226
– volume: 241
  start-page: 608
  year: 2012
  ident: bib66
  article-title: Developmental expression of Drosophila Wiskott-Aldrich syndrome family proteins
  publication-title: Developmental Dynamics
  doi: 10.1002/dvdy.23742
– volume: 290
  start-page: 10057
  year: 2015
  ident: bib71
  article-title: Conformational flexibility and subunit arrangement of the modular yeast Spt-Ada-Gcn5 acetyltransferase complex
  publication-title: Journal of Biological Chemistry
  doi: 10.1074/jbc.M114.624684
– volume: 156
  start-page: 689
  year: 2002
  ident: bib92
  article-title: SCAR is a primary regulator of Arp2/3-dependent morphological events in Drosophila
  publication-title: The Journal of Cell Biology
  doi: 10.1083/jcb.200109057
– volume: 83
  start-page: 490
  year: 2002
  ident: bib51
  article-title: Signaling mechanisms that regulate actin-based motility processes in the nervous system
  publication-title: Journal of Neurochemistry
  doi: 10.1046/j.1471-4159.2002.01185.x
– volume: 4
  year: 2013
  ident: bib43
  article-title: Separation of a functional deubiquitylating module from the SAGA complex by the proteasome regulatory particle
  publication-title: Nature Communications
  doi: 10.1038/ncomms3641
– volume: 341
  start-page: 1002
  year: 2013
  ident: bib52
  article-title: Nuclear Wave1 is required for reprogramming transcription in oocytes and for normal development
  publication-title: Science
  doi: 10.1126/science.1240376
– volume: 24
  start-page: 574
  year: 2010
  ident: bib53
  article-title: Linking H3K79 trimethylation to wnt signaling through a novel Dot1-containing complex (DotCom)
  publication-title: Genes & Development
  doi: 10.1101/gad.1898410
– volume: 7
  year: 2011
  ident: bib40
  article-title: Combinatorial depletion analysis to assemble the network architecture of the SAGA and ADA chromatin remodeling complexes
  publication-title: Molecular Systems Biology
  doi: 10.1038/msb.2011.40
– volume: 10
  start-page: 719
  year: 2006
  ident: bib93
  article-title: A Hedgehog-Induced BTB protein modulates hedgehog signaling by degrading ci/Gli transcription factor
  publication-title: Developmental Cell
  doi: 10.1016/j.devcel.2006.05.004
– volume: 12
  start-page: 293
  year: 2015
  ident: bib17
  article-title: VEGF signaling regulates cofilin and the Arp2/3-complex within the axonal growth cone
  publication-title: Current Neurovascular Research
  doi: 10.2174/1567202612666150603141144
– volume: 50
  start-page: 831
  year: 2013
  ident: bib24
  article-title: Mutations in the intellectual disability gene Ube2a cause neuronal dysfunction and impair parkin-dependent mitophagy
  publication-title: Molecular Cell
  doi: 10.1016/j.molcel.2013.04.012
– volume: 2
  start-page: 2
  year: 2009
  ident: bib39
  article-title: Yeast Sgf73/Ataxin-7 serves to anchor the deubiquitination module into both SAGA and slik(SALSA) HAT complexes
  publication-title: Epigenetics & Chromatin
  doi: 10.1186/1756-8935-2-2
– volume: 9
  start-page: 676
  year: 2012
  ident: bib70
  article-title: Fiji: an open-source platform for biological-image analysis
  publication-title: Nature Methods
  doi: 10.1038/nmeth.2019
– volume: 90
  start-page: 926
  year: 2011
  ident: bib28
  article-title: Controlling actin cytoskeletal organization and dynamics during neuronal morphogenesis
  publication-title: European Journal of Cell Biology
  doi: 10.1016/j.ejcb.2010.08.011
– volume: 29
  start-page: 99
  year: 2001
  ident: bib60
  article-title: Glial cells mediate target layer selection of retinal axons in the developing visual system of Drosophila
  publication-title: Neuron
  doi: 10.1016/S0896-6273(01)00183-0
– volume: 8
  start-page: 1347
  year: 1998
  ident: bib46
  article-title: Scar1 and the related Wiskott-Aldrich syndrome protein, WASP, regulate the actin cytoskeleton through the Arp2/3 complex
  publication-title: Current Biology
  doi: 10.1016/S0960-9822(98)00015-3
– volume: 723
  start-page: 257
  year: 2011
  ident: bib91
  article-title: Development of expression-ready constructs for generation of proteomic libraries
  publication-title: Methods in Molecular Biology
  doi: 10.1007/978-1-61779-043-0_17
– volume: 36
  start-page: 315
  year: 2004
  ident: bib59
  article-title: Mechanism of neurodegenerative disease: role of the ubiquitin proteasome system
  publication-title: Annals of Medicine
  doi: 10.1080/07853890410031948
– volume: 28
  start-page: 259
  year: 2014
  ident: bib54
  article-title: Loss of Drosophila Ataxin-7, a SAGA subunit, reduces H2B ubiquitination and leads to neural and retinal degeneration
  publication-title: Genes & Development
  doi: 10.1101/gad.225151.113
– volume: 18
  start-page: 565
  year: 2005
  ident: bib50
  article-title: Loss of HAUSP-mediated deubiquitination contributes to DNA damage-induced destabilization of hdmx and Hdm2
  publication-title: Molecular Cell
  doi: 10.1016/j.molcel.2005.04.024
– volume: 28
  start-page: 1999
  year: 2014
  ident: bib5
  article-title: The SAGA coactivator complex acts on the whole transcribed genome and is required for RNA polymerase II transcription
  publication-title: Genes & Development
  doi: 10.1101/gad.250225.114
– volume: 282
  start-page: 30150
  year: 2007
  ident: bib90
  article-title: Proteolytic cleavage of ataxin-7 by caspase-7 modulates cellular toxicity and transcriptional dysregulation
  publication-title: Journal of Biological Chemistry
  doi: 10.1074/jbc.M705265200
– volume: 6
  start-page: 28
  year: 2015
  ident: bib82
  article-title: Rho family GTPase functions in Drosophila epithelial wound repair
  publication-title: Small GTPases
  doi: 10.4161/21541248.2014.982415
– volume: 468
  start-page: 533
  year: 2010
  ident: bib10
  article-title: Structure and control of the actin regulatory WAVE complex
  publication-title: Nature
  doi: 10.1038/nature09623
– volume: 10
  start-page: 707
  year: 2008
  ident: bib29
  article-title: Yeast Ataxin-7 links histone deubiquitination with gene gating and mRNA export
  publication-title: Nature Cell Biology
  doi: 10.1038/ncb1733
– volume: 103
  start-page: 33
  year: 2006
  ident: bib84
  article-title: Evidence for the direct involvement of {beta}TrCP in Gli3 protein processing
  publication-title: PNAS
  doi: 10.1073/pnas.0509927103
– volume: 105
  start-page: 1473
  year: 1987
  ident: bib14
  article-title: Effects of cytochalasin and phalloidin on actin
  publication-title: The Journal of Cell Biology
  doi: 10.1083/jcb.105.4.1473
– volume: 32
  start-page: 318
  year: 2015
  ident: bib57
  article-title: Rac1 nucleocytoplasmic shuttling drives nuclear shape changes and tumor invasion
  publication-title: Developmental Cell
  doi: 10.1016/j.devcel.2014.12.019
– volume: 306
  start-page: 2084
  year: 2004
  ident: bib35
  article-title: Acetylation by Tip60 is required for selective histone variant exchange at DNA lesions
  publication-title: Science
  doi: 10.1126/science.1103455
– volume: 65
  year: 2012
  ident: bib62
  article-title: Ex vivo culturing of whole, developing Drosophila brains
  publication-title: JOVE
  doi: 10.3791/4270
– volume: 26
  start-page: 871
  year: 2006
  ident: bib23
  article-title: Host cell factor and an uncharacterized SANT domain protein are stable components of ATAC, a novel dAda2A/dGcn5-containing histone acetyltransferase complex in Drosophila
  publication-title: Molecular and Cellular Biology
  doi: 10.1128/MCB.26.3.871-882.2006
– volume: 6
  start-page: 2435
  year: 2016
  ident: bib45
  article-title: Transcriptome profiling identifies Multiplexin as a Target of SAGA Deubiquitinase Activity in Glia Required for Precise Axon Guidance During Drosophila Visual Development
  publication-title: G3: Genes|Genomes|Genetics
  doi: 10.1534/g3.116.031310
– volume: 14
  start-page: 229
  year: 1995
  ident: bib47
  article-title: Mutations disrupting neuronal connectivity in the Drosophila visual system
  publication-title: Neuron
  doi: 10.1016/0896-6273(95)90281-3
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Snippet Atxn7, a subunit of SAGA chromatin remodeling complex, is subject to polyglutamine expansion at the amino terminus, causing spinocerebellar ataxia type 7...
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SubjectTerms Actin
Actin Cytoskeleton - metabolism
Animals
Ataxia
Ataxin
Ataxin-7 - metabolism
Ataxin7
Cell Biology
Chromatin
Chromatin remodeling
Cytoskeleton
Disease
Drosophila melanogaster - physiology
Drosophila Proteins - metabolism
Endopeptidases - metabolism
Enzymes
Gene expression
Gene Expression Regulation
Genotype & phenotype
Haploinsufficiency
Insects
Localization
Mass spectrometry
Microfilament Proteins - metabolism
Mutation
Nervous system diseases
neurobiology
Neurodegeneration
Neurodegenerative diseases
Neuroscience
Non-stop
Peptides
Physiological aspects
Polyglutamine
Proteasomes
Protein Interaction Mapping
Protein Interaction Maps
Proteins
Retina
SAGA complex
Scientific imaging
Spinocerebellar ataxia
Spinocerebellar ataxias
Trinucleotide repeat diseases
WAVE regulatory complex
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Title Ataxin-7 and Non-stop coordinate SCAR protein levels, subcellular localization, and actin cytoskeleton organization
URI https://www.ncbi.nlm.nih.gov/pubmed/31348003
https://www.proquest.com/docview/2283089983
https://www.proquest.com/docview/2265777814
https://pubmed.ncbi.nlm.nih.gov/PMC6693919
https://doaj.org/article/6e9fff0a29524fd48b00d077b354eed6
Volume 8
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