A CCRK-EZH2 epigenetic circuitry drives hepatocarcinogenesis and associates with tumor recurrence and poor survival of patients

• Published in: Journal of hepatology Vol. 62; no. 5; pp. 1100 - 1111
• Main Authors: Feng, Hai, Yu, Zhuo, Tian, Yuan, Lee, Ying-Ying, Li, May S., Go, Minnie Y.Y., Cheung, Yue-Sun, Lai, Paul B.S., Chan, Andrew M.L., To, Ka-Fai, Chan, Henry L.Y., Sung, Joseph J.Y., Cheng, Alfred S.L.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.05.2015
• Subjects: Androgen receptor; Animals; Carcinogenesis - genetics; Carcinoma, Hepatocellular - genetics; Carcinoma, Hepatocellular - pathology; Cell Line, Tumor; Chromatin modifications; Cyclin-Dependent Kinases - genetics; Ectopic Gene Expression; Enhancer of Zeste Homolog 2 Protein; Epigenesis, Genetic; Gastroenterology and Hepatology; Gender disparity; Gene Expression Regulation, Neoplastic; Hepatocellular carcinoma; Humans; Kinase; Liver Neoplasms - genetics; Liver Neoplasms - pathology; Mice; MicroRNAs - genetics; Neoplasm Recurrence, Local - genetics; Polycomb Repressive Complex 2 - genetics; Signal Transduction - genetics; Survival Rate; RT; p-EZH2Ser21; CCRK; Hepatocellular carcinoma; dn; ChIP; dp; GSK-3β; shRNA; E2F1; KD; HBV; WT; p-AKTSer473; p-ARSer81; HCC; siRNA; DEN; AR; p-GSK3βSer9; PRC2; ARE; Kinase; TCF; Androgen receptor; Gender disparity; Chromatin modifications; EZH2; H3K27me3; hepatitis B virus; E2F transcription factor 1; T-cell factor; trimethylation of lysine 27 at histone H3; AKT phosphorylation at serine473; androgen receptor; p-EZH2 Ser21; hepatocellular carcinoma; short-hairpin RNA; diethylnitrosamine; kinase-defective; p-AR Ser81; GSK-3β phosphorylation at serine9; androgen-responsive element; glycogen synthase kinase 3β; dominant-positive; polycomb repressive complex 2; cell cycle-related kinase; enhancer of zeste homolog 2; reverse transcription; chromatin immunoprecipitation; dominant-negative; p-GSK3β Ser9; small-interfering RNA; p-AKT Ser473; AR phosphorylation at serine81; EZH2 phosphorylation at serine21; wild-type

[Display omitted] Aberrant chromatin modification is a key feature of hepatocellular carcinoma (HCC), which is characterized by strong sexual dimorphism. Both enhancer of zeste homolog 2 (EZH2) and cell cycle-related kinase (CCRK) contribute to hepatocarcinogenesis, yet whether the two oncogenic fac...