Effective removal of a range of Ti/Ri plasmids using a pBBR1-type vector having a repABC operon and a lux reporter system

• Published in: Applied microbiology and biotechnology Vol. 102; no. 4; pp. 1823 - 1836
• Main Authors: Yamamoto, Shinji, Sakai, Ayako, Agustina, Vita, Moriguchi, Kazuki, Suzuki, Katsunori
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.02.2018; Springer; Springer Nature B.V
• Subjects: Acetosyringone; Agrobacterium; Agrobacterium tumefaciens; Applied Genetics and Molecular Biotechnology; Backbone; Bacteria; Biomedical and Life Sciences; Biotechnology; Colonies; Evictions; Genetic aspects; Genetic vectors; Incompatibility; Life Sciences; Microbial Genetics and Genomics; Microbiology; Observations; Operons; Physiological aspects; Plasmids; Ribonucleic acid; RNA; Strains (organisms); Virulence; Waterborne diseases; Luciferase reporter; Plasmid curing; Ti plasmid; Plasmid incompatibility; Agrobacterium

Ti and Ri plasmids of pathogenic Agrobacterium strains are stably maintained by the function of a repABC operon and have been classified into four incompatibility groups, namely, incRh1, incRh2, incRh3, and incRh4. Removal of these plasmids from their bacterial cells is an important step in determin...