Functional insights from a surface antigen mRNA-bound proteome

• Published in: eLife Vol. 10
• Main Authors: Melo do Nascimento, Larissa, Egler, Franziska, Arnold, Katharina, Papavasiliou, Nina, Clayton, Christine, Erben, Esteban
• Format: Journal Article
• Language: English
• Published: England eLife Science Publications, Ltd 30.03.2021; eLife Sciences Publications Ltd; eLife Sciences Publications, Ltd
• Subjects: 3' Untranslated regions; Adaptive immunity; African trypanosomiasis; antigenic variation; Antigens; CFB2; Cyclin F; F-box; F-box protein; Gene expression; Glycoproteins; Mass spectrometry; Messenger RNA; Microbiology and Infectious Disease; mRNA stability; Pathogenicity; Physiological aspects; Protein binding; Protein purification; Proteins; Proteome - chemistry; Proteomes; Protozoan Proteins - chemistry; RNA polymerase; RNA Stability; RNA, Messenger - chemistry; RNA-binding protein; RNA-binding proteins; Scientific imaging; Tetracycline; Tetracyclines; Trypanosoma brucei; Trypanosoma brucei brucei - chemistry; Variant surface glycoprotein; Variant Surface Glycoproteins, Trypanosoma - chemistry; VSG; Canada; antigenic variation; F-box; infectious disease; microbiology; RNA-binding proteins; VSG; CFB2; Trypanosoma brucei

is the causative agent of human sleeping sickness. The parasites' variant surface glycoprotein (VSG) enables them to evade adaptive immunity via antigenic variation. VSG comprises 10% of total cell protein and the high stability of VSG mRNA is essential for trypanosome survival. To determine ho...