Bile cell‑free DNA as a novel and powerful liquid biopsy for detecting somatic variants in biliary tract cancer

• Published in: Oncology reports Vol. 42; no. 2; pp. 549 - 560
• Main Authors: Shen, Ningjia, Zhang, Dadong, Yin, Lei, Qiu, Yinghe, Liu, Jian, Yu, Wenlong, Fu, Xiaohui, Zhu, Bin, Xu, Xiaoya, Duan, Anqi, Chen, Zishuo, Wang, Xiang, Cao, Xinkai, Zhao, Teng, Zhou, Zisong, Yu, Lianghe, Qin, Hao, Fang, Zheng, Li, Jing‑Yu, Liu, Yuanjin, Xiong, Lei, Yuan, Bo, Li, Fugen, Zhang, Yongjie
• Format: Journal Article
• Language: English
• Published: Greece Spandidos Publications 01.08.2019; Spandidos Publications UK Ltd; D.A. Spandidos
• Subjects: Adult; Aged; Analysis; Antigens; Bile; Biliary tract cancer; Biliary Tract Neoplasms - classification; Biliary Tract Neoplasms - diagnosis; Biliary Tract Neoplasms - genetics; Biomarkers, Tumor - genetics; Cancer; Cancer genetics; Cell-Free Nucleic Acids - analysis; Cell-Free Nucleic Acids - genetics; Cholangiocarcinoma; Deoxyribonucleic acid; DNA; DNA Copy Number Variations; DNA, Neoplasm - analysis; DNA, Neoplasm - genetics; Female; Follow-Up Studies; Gallbladder; Gallbladder cancer; Genes; Genetic research; High-Throughput Nucleotide Sequencing; Humans; Liquid Biopsy - methods; Male; Middle Aged; Mutation; Patients; Prognosis; Tumors
• ISSN: 1021-335X; 1791-2431; 1791-2431
• DOI: 10.3892/or.2019.7177

Tissue sampling of biliary tract carcinomas (BTCs) for molecular characterization is challenging. The aim of this study was to investigate the possibility of identifying individual actionable mutations derived from bile cell‑free DNA (cfDNA) using targeted deep sequencing. Ten BTC patients, four wit...