Towards a Rapid-Turnaround Low-Depth Unbiased Metagenomics Sequencing Workflow on the Illumina Platforms

• Published in: Bioengineering (Basel) Vol. 10; no. 5; p. 520
• Main Authors: Koh, Winston Lian Chye, Poh, Si En, Lee, Chun Kiat, Chan, Tim Hon Man, Yan, Gabriel, Kong, Kiat Whye, Lau, Lalita, Lee, Wai Yip Thomas, Cheng, Clark, Hoon, Shawn, Seow, Yiqi
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 25.04.2023; MDPI
• Subjects: Bioengineering; Data analysis; Dengue fever; Deoxyribonucleic acid; Diagnostic systems; DNA; DNA sequencing; DNA/RNA library preparation; Gene sequencing; Genomes; host depletion; infectious disease; Infectious diseases; liquid biopsy; metagenomic sequencing; Metagenomics; Methods; Next-generation sequencing; Nucleotide sequencing; Pathogens; Plasma; Platforms; Ribonucleic acid; RNA; Severe acute respiratory syndrome coronavirus 2; Tropical diseases; Workflow; Singapore; United States > US; metagenomic sequencing; DNA/RNA library preparation; infectious disease; liquid biopsy; host depletion

Unbiased metagenomic sequencing is conceptually well-suited for first-line diagnosis as all known and unknown infectious entities can be detected, but costs, turnaround time and human background reads in complex biofluids, such as plasma, hinder widespread deployment. Separate preparations of DNA an...