Real-Time Monitoring of Cell Elasticity Reveals Oscillating Myosin Activity

• Published in: Biophysical journal Vol. 99; no. 11; pp. 3639 - 3646
• Main Authors: Schillers, Hermann, Wälte, Mike, Urbanova, Katarina, Oberleithner, Hans
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.12.2010; Biophysical Society; The Biophysical Society
• Subjects: Biophysics; Calcium; Calcium - metabolism; Cell Line; Cell Survival; Cells; Cytoskeleton; Dynamics; Elasticity; Epithelial Cells - cytology; Epithelial Cells - physiology; Humans; Membrane; Microscopy, Atomic Force - methods; Monitoring; Motors; Myosin; Myosin Type II - metabolism; Oscillations; Proteins; Real time; Time Factors
• ISSN: 0006-3495; 1542-0086
• DOI: 10.1016/j.bpj.2010.09.048

The cytoskeleton is the physical and biochemical interface for a large variety of cellular processes. Its complex regulation machinery is involved upstream and downstream in various signaling pathways. The cytoskeleton determines the mechanical properties of a cell. Thus, cell elasticity could serve as a parameter reflecting the behavior of the system rather than reflecting the specific properties of isolated components. In this study, we used atomic force microscopy to perform real-time monitoring of cell elasticity unveiling cytoskeletal dynamics of living bronchial epithelial cells. In resting cells, we found a periodic activity of the cytoskeleton. Amplitude and frequency of this spontaneous oscillation were strongly affected by intracellular calcium. Experiments reveal that basal cell elasticity and superimposed elasticity oscillations are caused by the collective action of myosin motor proteins. We characterized the cell as a mechanically multilayered structure, and followed cytoskeletal dynamics in the different layers with high time resolution. In conclusion, the collective activities of the myosin motor proteins define overall mechanical cell dynamics, reflecting specific changes of the chemical and mechanical environment.