Identification of substituent groups and related genes involved in salecan biosynthesis in Agrobacterium sp. ZX09

• Published in: Applied microbiology and biotechnology Vol. 101; no. 2; pp. 585 - 598
• Main Authors: Xu, Linxiang, Cheng, Rui, Li, Jing, Wang, Yang, Zhu, Bin, Ma, Shihong, Zhang, Weiming, Dong, Wei, Wang, Shiming, Zhang, Jianfa
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.01.2017; Springer; Springer Nature B.V
• Subjects: Agrobacterium; Agrobacterium - genetics; Agrobacterium - metabolism; Analysis; Bacteria; beta-Glucans - chemistry; beta-Glucans - metabolism; bioactive properties; Biomedical and Life Sciences; Biosynthesis; Biosynthetic Pathways - genetics; Biotechnological Products and Process Engineering; Biotechnology; Cellular biology; Chromatography, High Pressure Liquid; Cloning; Cloning, Molecular; enzymatic hydrolysis; Enzymes; Gene Deletion; Gene expression; Genes; Genetic aspects; Genetic Complementation Test; Genetic engineering; Glucans; high performance liquid chromatography; Life Sciences; Liquid chromatography; Magnetic Resonance Spectroscopy; Mass Spectrometry; Microbial Genetics and Genomics; Microbiology; Molecular weight; Multigene Family; mutants; NMR; Nuclear magnetic resonance; nuclear magnetic resonance spectroscopy; Observations; Physiological aspects; plasmids; Proteobacteria; Pyruvates - chemistry; Pyruvates - metabolism; Rheology; salt tolerance; Studies; Succinates - chemistry; Succinates - metabolism; Transferases - genetics; Viscosity; Gene cluster; Substituent; sp. ZX09; Salecan; Rheology; Agrobacterium sp. ZX09

Salecan, a soluble β-1,3- d -glucan produced by a salt-tolerant strain Agrobacterium sp. ZX09, has been the subject of considerable interest in recent years because of its multiple bioactivities and unusual rheological properties in solution. In this study, both succinyl and pyruvyl substituent grou...