Control of SARS-CoV-2 infection after Spike DNA or Spike DNA+Protein co-immunization in rhesus macaques

• Published in: PLoS pathogens Vol. 17; no. 9; p. e1009701
• Main Authors: Rosati, Margherita, Agarwal, Mahesh, Hu, Xintao, Devasundaram, Santhi, Stellas, Dimitris, Chowdhury, Bhabadeb, Bear, Jenifer, Burns, Robert, Donohue, Duncan, Pessaint, Laurent, Andersen, Hanne, Lewis, Mark G., Terpos, Evangelos, Dimopoulos, Meletios Athanasios, Wlodawer, Alexander, Mullins, James I., Venzon, David J., Pavlakis, George N., Felber, Barbara K.
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 22.09.2021; Public Library of Science (PLoS)
• Subjects: Animal models; Animals; Antibodies; Antigens; Binding; Biology and Life Sciences; Clinical trials; Cohort Studies; COVID-19; COVID-19 - immunology; COVID-19 - therapy; Deoxyribonucleic acid; Disease Models, Animal; DNA; DNA vaccines; DNA, Viral - immunology; Electroporation; Female; Health aspects; HIV; Human immunodeficiency virus; Immune response; Immune response (cell-mediated); Immune response (humoral); Immune system; Immunization; Immunization, Passive; Immunogenicity; Immunological research; Infections; Leukocytes, Mononuclear - immunology; Lymphocytes; Lymphocytes T; Macaca mulatta; Medicine and Health Sciences; Mice; mRNA; Mucosa; Mutation; Neutralizing; Phagocytosis; Plasmids; Proteins; Research and Analysis Methods; Respiratory tract; Response rates; RNA, Messenger - analysis; SARS-CoV-2 - genetics; SARS-CoV-2 - immunology; Severe acute respiratory syndrome; Severe acute respiratory syndrome coronavirus 2; Spike Glycoprotein, Coronavirus - genetics; Spike Glycoprotein, Coronavirus - immunology; T-Lymphocytes - immunology; Trachea; Vaccines; Vaccines, DNA - administration & dosage; Vaccines, DNA - immunology; Variance analysis; Vectors (Biology); Viral diseases; Viral proteins; Viruses; United States; United States > US

The speed of development, versatility and efficacy of mRNA-based vaccines have been amply demonstrated in the case of SARS-CoV-2. DNA vaccines represent an important alternative since they induce both humoral and cellular immune responses in animal models and in human trials. We tested the immunogen...