High glucose modulates IL-6 mediated immune homeostasis through impeding neutrophil extracellular trap formation

• Published in: FEBS letters Vol. 587; no. 14; pp. 2241 - 2246
• Main Authors: Joshi, Manjunath B., Lad, Apurva, Bharath Prasad, Alevoor S., Balakrishnan, Aswath, Ramachandra, Lingadakai, Satyamoorthy, Kapaettu
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 11.07.2013
• Subjects: 2-deoxy-d-glucose; 2-DG; Cells, Cultured; CGD; Chronic Granulomatous disease; Deoxyglucose - pharmacology; Diabetes; Diabetes Mellitus, Type 2 - immunology; Diabetes Mellitus, Type 2 - metabolism; energy; glucose; Glucose - physiology; Glycolysis; homeostasis; Homeostasis - immunology; Humans; Hyperglycemia; Hyperglycemia - immunology; Hyperglycemia - metabolism; IL-6; Immunity, Innate; Innate immunity; Interleukin-6; Interleukin-6 - physiology; l-NAME; lipopolysaccharide; Lipopolysaccharides - pharmacology; LPS; Male; Microbial Viability; N G-nitro-l-arginine methyl ester; NADPH; NCM; NETs; Neutrophil Activation; neutrophil culture medium; Neutrophil extracellular trap; neutrophil extracellular traps; neutrophils; Neutrophils - drug effects; Neutrophils - immunology; nicotinamide adenine dinucleotide phosphate; nitric oxide synthase; NOS; pathogens; phorbol myristate acetate; PMA; T2D; Tetradecanoylphorbol Acetate - pharmacology; TNF-α; Tumor necrosis factor-alpha; type 2 diabetes; NADPH; CGD; l-NAME; PMA; T2D; 2-DG; Innate immunity; IL-6; LPS; Interleukin-6; NETs; Hyperglycemia; NOS; TNF-α; Neutrophil extracellular trap; NCM; Diabetes

•High glucose impedes and delays NET formation.•Neutrophils from diabetic subjects respond weakly to NETosis.•Hyperglycemia induces a constitutively active pro-inflammatory condition in neutrophils.•IL-6 is a potent inducer of NETs and effects are abrogated by high glucose. Neutrophils serve as an a...