Identification of the protein coding capability of coronavirus defective viral genomes by mass spectrometry

• Published in: Virology journal Vol. 20; no. 1; p. 290
• Main Authors: Lin, Ching-Hung, Hsieh, Feng-Cheng, Lai, Chien-Chen, Wang, Wei-Chen, Kuo, Cheng-Yu, Yang, Chun-Chun, Hsu, Hsuan-Wei, Tam, Hon-Man-Herman, Yang, Cheng-Yao, Wu, Hung-Yi
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 07.12.2023; BioMed Central; BMC
• Subjects: Amino acids; Chromatography; Chromatography, Liquid; Coronavirus; Coronavirus - genetics; Coronavirus Infections; Coronaviruses; Defective viral genome; Dengue fever; Development and progression; Disease transmission; Gene expression; Genetic aspects; Genome, Viral; Genomes; Humans; Infections; Influenza; Liquid chromatography; Lysates; Mass spectrometry; Mass spectroscopy; nanopores; Pathogenesis; Peptides; Protein coding; Proteins; Proteins - genetics; Ribonucleic acid; RNA; RNA, Viral - genetics; Scientific imaging; Severe acute respiratory syndrome coronavirus 2; Tandem Mass Spectrometry; viral genome; Viral proteins; Virus research; Viruses; Taiwan; United States > US; Protein coding; Defective viral genome; Coronavirus; Gene expression; Pathogenesis

During coronavirus infection, in addition to the well-known coronavirus genomes and subgenomic mRNAs, an abundance of defective viral genomes (DVGs) can also be synthesized. In this study, we aimed to examine whether DVGs can encode proteins in infected cells. Nanopore direct RNA sequencing and liqu...