Accelerator Mass Spectrometry in Biomedical Dosimetry: Relationship Between Low-Level Exposure and Covalent Binding of Heterocyclic Amine Carcinogens to DNA

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 87; no. 14; pp. 5288 - 5292
• Main Authors: Turteltaub, K. W., Felton, J. S., Gledhill, B. L., Vogel, J. S., Southon, J. R., Caffee, M. W., Finkel, R. C., Nelson, D. E., Proctor, I. D., Davis, J. C.
• Format: Journal Article
• Language: English
• Published: Washington, DC National Academy of Sciences of the United States of America 01.07.1990; National Acad Sciences
• Subjects: 550201 - Biochemistry- Tracer Techniques; ADDUCTS; AMINES; Analytical biochemistry: general aspects, technics, instrumentation; Analytical, structural and metabolic biochemistry; ANIMALS; BACTERIA; BASIC BIOLOGICAL SCIENCES; Biological and medical sciences; BODY; Body weight; CARBON 14 COMPOUNDS; Carbon Radioisotopes; CARCINOGENS; Carcinogens - metabolism; CHRONIC EXPOSURE; DIGESTIVE SYSTEM; Dioxins - metabolism; DNA; DNA - isolation & purification; DNA - metabolism; DNA ADDUCTS; Dosage; Dose-Response Relationship, Drug; DOSE-RESPONSE RELATIONSHIPS; Fundamental and applied biological sciences. Psychology; GLANDS; Isotopes; Kinetics; LABELLED COMPOUNDS; LIVER; Liver - metabolism; Male; MAMMALS; MAN; MASS SPECTROMETERS; Mass Spectrometry - methods; MASS SPECTROSCOPY; MEASURING INSTRUMENTS; MICE; Mice, Inbred C57BL; MICROORGANISMS; Nucleotides; ORGANIC COMPOUNDS; ORGANS; Phosphorus Radioisotopes; Polychlorinated Dibenzodioxins - metabolism; POLYCYCLIC AROMATIC AMINES; PRIMATES; Quinoxalines - metabolism; Radiocarbon; RODENTS; SENSITIVITY; SPECTROMETERS; SPECTROSCOPY; VERTEBRATES; Quinoxaline derivatives; Radiolabelling; Liver; Rodentia; Cytotoxicity; Carcinogen; Vertebrata; Mammalia; Methylosinus trichosporium; Mouse; DNA; Molecular association; Covalent bond; Bacteria; Mass spectrometry; Molecular adduct; Physicochemical properties; Quantitative analysis

Accelerator mass spectrometry (AMS) is used to determine the amount of carcinogen covalently bound to mouse liver DNA (DNA adduct) following very low-level exposure to a14C-labeled carcinogen. AMS is a highly sensitive method for counting long-lived but rare cosmogenic isotopes. While AMS is a tool...