Integration of CpG-free DNA induces de novo methylation of CpG islands in pluripotent stem cells

• Published in: Science (American Association for the Advancement of Science) Vol. 356; no. 6337; pp. 503 - 508
• Main Authors: Takahashi, Yuta, Wu, Jun, Suzuki, Keiichiro, Martinez-Redondo, Paloma, Li, Mo, Liao, Hsin-Kai, Wu, Min-Zu, Hernández-Benítez, Reyna, Hishida, Tomoaki, Shokhirev, Maxim Nikolaievich, Esteban, Concepcion Rodriguez, Sancho-Martinez, Ignacio, Belmonte, Juan Carlos Izpisua
• Format: Journal Article
• Language: English
• Published: United States American Association for the Advancement of Science 05.05.2017; The American Association for the Advancement of Science
• Subjects: Aberration; Cancer; CpG Islands; Deoxyribonucleic acid; Differentiation; Disorders; DNA; DNA - metabolism; DNA Methylation; DNA Mismatch Repair - genetics; DNA repair; DNA Repair - genetics; Editing; Epigenesis, Genetic; Gene expression; Gene sequencing; Genomes; Genomic imprinting; Humans; Individualized Instruction; Insertion; Integration; Islands; Mammals; Methylation; Mismatch repair; MLH1 protein; Mutagenesis, Insertional; MutL Protein Homolog 1 - genetics; Neurons - metabolism; Nucleotide sequence; Pluripotency; Pluripotent Stem Cells - metabolism; Proteins; Purines; Pyrimidines; Stem cells; Ubiquitin-Protein Ligases - genetics

CpG islands (CGIs) are primarily promoter-associated genomic regions and are mostly unmethylated within highly methylated mammalian genomes. The mechanisms by which CGIs are protected from de novo methylation remain elusive. Here we show that insertion of CpG-free DNA into targeted CGIs induces de n...