Improvement of xylanase production by Aspergillus niger XY-1 using response surface methodology for optimizing the medium composition

• Published in: Journal of Zhejiang University. B. Science Vol. 9; no. 7; pp. 558 - 566
• Main Authors: Xu, Yao-xing, Li, Yan-li, Xu, Shao-chun, Liu, Yong, Wang, Xin, Tang, Jiang-wu
• Format: Journal Article
• Language: English
• Published: Heidelberg SP Zhejiang University Press 01.07.2008; Springer Nature B.V; Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China; Zhejiang University Press
• Subjects: Aspergillus niger; Aspergillus niger - enzymology; Biomedical and Life Sciences; Biomedicine; Biotechnology and Biochemistry; CCD; Culture Media; Endo-1,4-beta Xylanases - biosynthesis; Fermentation; Nitrogen - metabolism; PBD; Regression analysis; Studies; Triticum aestivum; 介质复合物; 曲霉菌; 木聚糖酶; Q55; Xylanase; Response surface methodology (RSM); Optimization of medium composition; Plackett-Burman design (PBD); Central composite design (CCD); XY-1; Aspergillus niger XY-1
• ISSN: 1673-1581; 1862-1783
• DOI: 10.1631/jzus.B0820038

Objective： To study the optimal medium composition for xylanase production by Aspergillus niger XY-1 in solid-state fermentation （SSF）. Methods： Statistical methodology including the Plackett-Burman design （PBD） and the central composite design （CCD） was employed to investigate the individual crucial component of the medium that significantly affected the enzyme yield. Results： Firstly, NaNO3, yeast extract, urea, Na2CO3, MgSO4, peptone and （NH4）2SO4 were screened as the significant factors positively affecting the xylanase production by PBD. Secondly, by valuating the nitrogen sources effect, urea was proved to be the most effective and economic nitrogen source for xylanase production and used for further optimization. Finally, the CCD and response surface methodology （RSM） were applied to determine the optimal concentration of each significant variable, which included urea, Na2CO3 and MgSO4. Subsequently a second-order polynomial was determined by multiple regression analysis. The optimum values of the critical components for maximum xylanase production were obtained as follows： x1 （urea）=0.163 （41.63 g/L）, x2 （Na2CO3）=-1.68 （2.64 g/L）, X3 （MgSO4）= 1.338 （10.68 g/L） and the predicted xylanase value was 14374.6 U/g dry substrate. Using the optimized condition, xylanase production by Aspergillus niger XY-1 after 48 h fermentation reached 14637 U/g dry substrate with wheat bran in the shake flask. Conclusion： By using PBD and CCD, we obtained the optimal composition for xylanase production by Aspergillus niger XY-1 in SSF, and the results of no additional expensive medium and shortened fermentation time for higher xylanase production show the potential for industrial utilization.