Multilocus analysis of Gallid herpesvirus 1 in layer chickens in Iraq

• Published in: Veterinary World Vol. 13; no. 1; pp. 170 - 176
• Main Author: Al-Saadi, Mohammed Hamzah Abdulkadhim
• Format: Journal Article
• Language: English
• Published: India Veterinary World 01.01.2020
• Subjects: Biodiversity; Chickens; Diseases; EDTA; Epidemiologists; gallid herpesvirus 1; Genes; Genetic diversity; infectious laryngotracheitis; infectious laryngotracheitis virus; Laryngotracheitis; Lung diseases; Molecular weight; Polymerase chain reaction; Poultry; Production management; Prophylaxis; Recombination; Respiratory diseases; Respiratory tract diseases; Reversion; Strains (organisms); Vaccines; Viruses; Western blotting; United Kingdom; Iraq; Gallid herpesvirus 1; infectious laryngotracheitis; infectious laryngotracheitis virus; polymerase chain reaction
• ISSN: 0972-8988; 2231-0916
• DOI: 10.14202/vetworld.2020.170-176

Infectious laryngotracheitis virus (ILTV) causes a highly pathogenic respiratory disease that affects poultry. It is also known as 1. ILT prophylaxis measures often include using live attenuated vaccines. The live attenuated vaccine can, however, lead to the formation of new strains of ILTV as a result of vaccine reversion and recombination with field strains. Therefore, this study was performed to explore the multilocus variation of ILTV strains of field and vaccine origin. Samples were tested from two distinctive geographical areas in Iraq as little is known about the ILTV genetic diversity within these areas. The polymerase chain reaction method was utilized to generate sequencing templates of six highly polymorphic genes, including UL54, UL52, gB, ICP18.5, ICP4, and gJ in the layer chicken sample (n=15). The Western blotting technique was also employed to detect and estimate the native molecular weight of gE. The results revealed an important degree of genetic relatedness between the field and vaccine strains across all genes. In addition, gE was found to be expressed natively at 49 kDa. The findings of this study may be used to improve the production process of the vaccine for more effective ILT prophylaxis and could further the understanding of epidemiologists and immunologists to better control ILT in the future.