NaLi-H1: A universal synthetic library of humanized nanobodies providing highly functional antibodies and intrabodies

• Published in: eLife Vol. 5
• Main Authors: Moutel, Sandrine, Bery, Nicolas, Bernard, Virginie, Keller, Laura, Lemesre, Emilie, de Marco, Ario, Ligat, Laetitia, Rain, Jean-Christophe, Favre, Gilles, Olichon, Aurélien, Perez, Franck
• Format: Journal Article
• Language: English
• Published: England eLife Sciences Publications Ltd 19.07.2016; eLife Sciences Publication; eLife Sciences Publications, Ltd
• Subjects: Actin; Animals; Antibodies; Antibodies, Monoclonal, Humanized; Antigen (tumor-associated); Antigens; Biochemistry, Molecular Biology; Camelidae; Camelids, New World; Cancer; Cell Biology; Cloning; Flow cytometry; Guanosine triphosphatases; Humans; Immunization; Immunoglobulins; Immunology; Intrabodies; Life Sciences; Molecular Biology - methods; Nanobodies; p53 Protein; Peptide Library; Phage display; Proteins; recombinant antibodies; Single-Domain Antibodies; synthetic library; Therapeutic applications; Tools and Resources; Tubulin; Tumor cells; mouse; cell biology; phage display; intrabodies; recombinant antibodies; human; immunology; synthetic library; E. coli

In vitro selection of antibodies allows to obtain highly functional binders, rapidly and at lower cost. Here, we describe the first fully synthetic phage display library of humanized llama single domain antibody (NaLi-H1: Nanobody Library Humanized 1). Based on a humanized synthetic single domain an...