A Label-Free, Quadruplex-Based Functional Molecular Beacon (LFG4-MB) for Fluorescence Turn-On Detection of DNA and Nuclease

• Published in: Chemistry : a European journal Vol. 17; no. 5; pp. 1635 - 1641
• Main Authors: Hu, Dan, Huang, Zhenzhen, Pu, Fang, Ren, Jinsong, Qu, Xiaogang
• Format: Journal Article
• Language: English
• Published: Weinheim WILEY-VCH Verlag 01.02.2011; WILEY‐VCH Verlag; Wiley Subscription Services, Inc
• Subjects: Base Sequence; Beacons; Binders; Biological; Biological Assay; Catalysts; Chemical modification; Chemistry; Deoxyribonucleases - chemistry; Deoxyribonucleases - metabolism; Deoxyribonucleic acid; DNA; DNA - chemistry; DNA recognition; Fluorescence; Fluorescent Dyes - chemistry; fluorescent probes; G-quadruplex; G-Quadruplexes; Gene sequencing; Kinetics; molecular beacon; Molecular Structure; Nuclease; Nucleic Acid Conformation; Nucleotide sequence; Recognition; Sensitivity and Specificity; Staining and Labeling; Uracil

We demonstrate a novel concept for the construction of a label‐free, quadruplex‐based functional molecular beacon (LFG4‐MB) by using G‐quadruplex motif as a substitute for Watson–Crick base pairing in the MB stem and a specific G‐quadruplex binder, N‐methyl mesoporphyrin IX (NMM) as a reporter. It s...