Membrane topology and multimeric structure of a mechanosensitive channel protein of Escherichia coli

• Published in: The EMBO journal Vol. 15; no. 18; pp. 4798 - 4805
• Main Authors: Blount, P., Sukharev, S. I., Moe, P. C., Schroeder, M. J., Guy, H. R., Kung, C.
• Format: Journal Article
• Language: English
• Published: England 16.09.1996
• Subjects: Alkaline Phosphatase - genetics; Alkaline Phosphatase - metabolism; Amino Acid Sequence; Cytoplasm - chemistry; Deoxyribonuclease BamHI - metabolism; Escherichia coli; Escherichia coli Proteins; Histidine - metabolism; Intracellular Membranes - chemistry; Ion Channels - chemistry; Ion Channels - genetics; Ion Channels - physiology; Kinetics; Liposomes - metabolism; Molecular Sequence Data; Mutagenesis, Site-Directed; Protein Conformation; Structure-Activity Relationship; Non-programmatic

We have studied the membrane topology and multimeric structure of a mechanosensitive channel, MscL, which we previously isolated and cloned from Escherichia coli. We have localized this 15‐kDa protein to the inner membrane and, by PhoA fusion, have shown that it contains two transmembrane domains wi...