Functional verification of the JmLFY gene associated with the flowering of Juglans mandshurica Maxim

• Published in: PeerJ (San Francisco, CA) Vol. 11; p. e14938
• Main Authors: Cai, Jiayou, Jia, Ruoxue, Jiang, Ying, Fu, Jingqi, Dong, Tianyi, Deng, Jifeng, Zhang, Lijie
• Format: Journal Article
• Language: English
• Published: United States PeerJ. Ltd 07.03.2023; PeerJ, Inc; PeerJ Inc
• Subjects: Agricultural Science; Arabidopsis - genetics; Arabidopsis thaliana; Children; Cloning; Flowering; Flowers & plants; Flowers - genetics; Functional verification; Gene expression; Genetic aspects; Genetic engineering; Genetic research; Genetic transformation; Genetically modified plants; Health aspects; Infections; Inflorescence - genetics; JmLFY gene; Juglans; Juglans mandshurica; Juglans mandshurica Maxim; Leaves; Molecular Biology; Mutants; Plant Breeding; Plant Science; Seeds; Transgenic plants; China; Beijing China; Juglans mandshurica Maxim; JmLFY gene; Functional verification

In this study, a pBI121- plant expression vector was constructed on the basis of obtaining the full-length sequence of the gene from , which was then used for genetic transformation inflorescence infection using wild-type and mutants as transgenic receptors. Seeds of positive plants with high expres...