Functional verification of the JmLFY gene associated with the flowering of Juglans mandshurica Maxim

In this study, a pBI121- plant expression vector was constructed on the basis of obtaining the full-length sequence of the gene from , which was then used for genetic transformation inflorescence infection using wild-type and mutants as transgenic receptors. Seeds of positive plants with high expres...

Full description

Saved in:
Bibliographic Details
Published inPeerJ (San Francisco, CA) Vol. 11; p. e14938
Main Authors Cai, Jiayou, Jia, Ruoxue, Jiang, Ying, Fu, Jingqi, Dong, Tianyi, Deng, Jifeng, Zhang, Lijie
Format Journal Article
LanguageEnglish
Published United States PeerJ. Ltd 07.03.2023
PeerJ, Inc
PeerJ Inc
Subjects
Agricultural Science
Arabidopsis - genetics
Arabidopsis thaliana
Children
Cloning
Flowering
Flowers & plants
Flowers - genetics
Functional verification
Gene expression
Genetic aspects
Genetic engineering
Genetic research
Genetic transformation
Genetically modified plants
Health aspects
Infections
Inflorescence - genetics
JmLFY gene
Juglans
Juglans mandshurica
Juglans mandshurica Maxim
Leaves
Molecular Biology
Mutants
Plant Breeding
Plant Science
Seeds
Transgenic plants
China
Beijing China
Juglans mandshurica Maxim
JmLFY gene
Functional verification
Online AccessGet full text

Cover

Abstract In this study, a pBI121- plant expression vector was constructed on the basis of obtaining the full-length sequence of the gene from , which was then used for genetic transformation inflorescence infection using wild-type and mutants as transgenic receptors. Seeds of positive plants with high expression of were collected and sowed till the homozygous T3 regeneration plants were obtained. Then the expression of flowering-related genes ( , , and ) in T3 generation plants were analyzed and the results showed that gene overexpression promoted the expression of flowering-related genes and resulted in earlier flowering in . The plants of -transformed and -transformed mutants appeared shorter leaves, longer fruit pods, and fewer cauline leaves than those of wild-type and the mutants plants, respectively. In addition, some secondary branches in the transgenic plants converted into inflorescences, which indicated that the overexpression of promoted the transition from vegetative growth to reproductive growth, and compensate the phenotypic defects of mutant partially. The results provides a scientific reference for formulating reasonable genetic improvement strategies such as shortening childhood, improving yield and quality, and breeding desirable varieties, which have important guiding significance in production.
AbstractList In this study, a pBI121-JmLFY plant expression vector was constructed on the basis of obtaining the full-length sequence of the JmLFY gene from Juglans mandshurica, which was then used for genetic transformation via Agrobacterium inflorescence infection using wild-type Arabidopsis thaliana and lfy mutants as transgenic receptors. Seeds of positive A. thaliana plants with high expression of JmLFY were collected and sowed till the homozygous T3 regeneration plants were obtained. Then the expression of flowering-related genes (AtAP1, AtSOC1, AtFT and AtPI) in T3 generation plants were analyzed and the results showed that JmLFY gene overexpression promoted the expression of flowering-related genes and resulted in earlier flowering in A. thaliana. The A. thaliana plants of JmLFY-transformed and JmLFY-transformed lfy mutants appeared shorter leaves, longer fruit pods, and fewer cauline leaves than those of wild-type and the lfy mutants plants, respectively. In addition, some secondary branches in the transgenic plants converted into inflorescences, which indicated that the overexpression of JmLFY promoted the transition from vegetative growth to reproductive growth, and compensate the phenotypic defects of lfy mutant partially. The results provides a scientific reference for formulating reasonable genetic improvement strategies such as shortening childhood, improving yield and quality, and breeding desirable varieties, which have important guiding significance in production.
In this study, a pBI121- JmLFY plant expression vector was constructed on the basis of obtaining the full-length sequence of the JmLFY gene from Juglans mandshurica , which was then used for genetic transformation via Agrobacterium inflorescence infection using wild-type Arabidopsis thaliana and lfy mutants as transgenic receptors. Seeds of positive A. thaliana plants with high expression of JmLFY were collected and sowed till the homozygous T3 regeneration plants were obtained. Then the expression of flowering-related genes ( AtAP1 , AtSOC1 , AtFT and AtPI ) in T3 generation plants were analyzed and the results showed that JmLFY gene overexpression promoted the expression of flowering-related genes and resulted in earlier flowering in A. thaliana . The A. thaliana plants of JmLFY -transformed and JmLFY -transformed lfy mutants appeared shorter leaves, longer fruit pods, and fewer cauline leaves than those of wild-type and the lfy mutants plants, respectively. In addition, some secondary branches in the transgenic plants converted into inflorescences, which indicated that the overexpression of JmLFY promoted the transition from vegetative growth to reproductive growth, and compensate the phenotypic defects of lfy mutant partially. The results provides a scientific reference for formulating reasonable genetic improvement strategies such as shortening childhood, improving yield and quality, and breeding desirable varieties, which have important guiding significance in production.
In this study, a pBI121- plant expression vector was constructed on the basis of obtaining the full-length sequence of the gene from , which was then used for genetic transformation inflorescence infection using wild-type and mutants as transgenic receptors. Seeds of positive plants with high expression of were collected and sowed till the homozygous T3 regeneration plants were obtained. Then the expression of flowering-related genes ( , , and ) in T3 generation plants were analyzed and the results showed that gene overexpression promoted the expression of flowering-related genes and resulted in earlier flowering in . The plants of -transformed and -transformed mutants appeared shorter leaves, longer fruit pods, and fewer cauline leaves than those of wild-type and the mutants plants, respectively. In addition, some secondary branches in the transgenic plants converted into inflorescences, which indicated that the overexpression of promoted the transition from vegetative growth to reproductive growth, and compensate the phenotypic defects of mutant partially. The results provides a scientific reference for formulating reasonable genetic improvement strategies such as shortening childhood, improving yield and quality, and breeding desirable varieties, which have important guiding significance in production.
ArticleNumber e14938
Audience Academic
Author Dong, Tianyi
Jia, Ruoxue
Jiang, Ying
Zhang, Lijie
Cai, Jiayou
Fu, Jingqi
Deng, Jifeng
Author_xml – sequence: 1
  givenname: Jiayou
  surname: Cai
  fullname: Cai, Jiayou
  organization: Key Laboratory of Forest Tree Genetics, Breeding and Cultivation of Liaoning Province, Shenyang, Liaoning, China
– sequence: 2
  givenname: Ruoxue
  surname: Jia
  fullname: Jia, Ruoxue
  organization: Shenyang Agricultural University, Shenyang, Liaoning, China
– sequence: 3
  givenname: Ying
  surname: Jiang
  fullname: Jiang, Ying
  organization: Shenyang Agricultural University, Shenyang, Liaoning, China
– sequence: 4
  givenname: Jingqi
  surname: Fu
  fullname: Fu, Jingqi
  organization: Key Laboratory of Forest Tree Genetics, Breeding and Cultivation of Liaoning Province, Shenyang, Liaoning, China
– sequence: 5
  givenname: Tianyi
  surname: Dong
  fullname: Dong, Tianyi
  organization: Key Laboratory of Forest Tree Genetics, Breeding and Cultivation of Liaoning Province, Shenyang, Liaoning, China
– sequence: 6
  givenname: Jifeng
  orcidid: 0000-0001-5496-5009
  surname: Deng
  fullname: Deng, Jifeng
  organization: Key Laboratory of Forest Tree Genetics, Breeding and Cultivation of Liaoning Province, Shenyang, Liaoning, China
– sequence: 7
  givenname: Lijie
  surname: Zhang
  fullname: Zhang, Lijie
  organization: Key Laboratory of Forest Tree Genetics, Breeding and Cultivation of Liaoning Province, Shenyang, Liaoning, China
BackLink https://www.ncbi.nlm.nih.gov/pubmed/36908820$$D View this record in MEDLINE/PubMed
BookMark eNptkk1v1DAQhiNUREvpiTuKhISQ0C7-iBP7VFUVC60WcYEDJ8txxolXib3YSQv_Hme3lF2EfbA9fua1Zvw-z06cd5BlLzFaVhWu3m8BwmaJC0H5k-yM4LJacMrEycH-NLuIcYPS4KREnD7LTmkpEOcEnWXNanJ6tN6pPr-DYI3Vaj7m3uRjB_ntsF59z1twkKsYvbZqhCa_t2O3uza9v09Zrp3526ntlYv5oFwTuykkqfyz-mmHF9lTo_oIFw_refZt9eHr9afF-svHm-ur9UKzqhgXAASULjVlIEhR15UpldG1YkWNBSpLwmsjcFFQXVCSgqzCxpSplKZGiEFFz7ObvW7j1UZugx1U-CW9snIX8KGVKoxW9yApIYJwRDXTqOC1qEvFagNYCd0wxJukdbnX2k71AI0GNwbVH4ke3zjbydbfSTx3miKWFN4-KAT_Y4I4ysFGDX3qEfgpSlLxkmGUqkjo63_QjZ9C-pMdVWBUpPmXalWqwDrj08N6FpVXFRWcCkFmavkfKs0GBquTe4xN8aOENwcJHah-7KLvp9kG8Rh8twd18DEGMI_dwEjObpQ7N8qdGxP96rCBj-wf79HfIeTbJg
CitedBy_id crossref_primary_10_3389_fpls_2023_1243030
Cites_doi 10.1093/jxb/erl081
10.1016/j.scienta.2022.110885
10.13271/j.mpb.017.000396
10.3390/genes11030319
10.1007/s004290050103
10.1038/srep41124
10.1104/pp.117.1.1
10.1186/s12870-020-02372-w
10.22059/IJHST.2018.260944.249
10.1016/j.scienta.2019.109014
10.1186/s12864-017-4402-2
10.1007/BF00041177
10.1093/pcp/pce143
10.1186/s12864-018-5356-8
10.1111/j.1525-142X.2007.00182.x
10.1007/978-1-4757-9607-0_17
10.1105/tpc.3.8.771
10.1016/j.copbio.2014.11.023
10.16420/j.issn.0513-353x.2018-0023
10.1007/s11103-008-9330-8
10.1038/s41438-021-00550-x
10.15835/nbha46211183
10.1105/tpc.104.025353
10.1038/85719
10.1007/s00425-005-0086-y
10.2135/cropsci2008.03.0118
10.1590/S1516-05722009000400015
10.1007/s11703-011-1095-1
10.1023/a:1008992719010
10.1093/jxb/eraa161
10.1038/srep37306
10.1111/nph.16490
ContentType Journal Article
Copyright 2023 Cai et al.
COPYRIGHT 2023 PeerJ. Ltd.
2023 Cai et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.
2023 Cai et al. 2023 Cai et al.
Copyright_xml – notice: 2023 Cai et al.
– notice: COPYRIGHT 2023 PeerJ. Ltd.
– notice: 2023 Cai et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.
– notice: 2023 Cai et al. 2023 Cai et al.
DBID CGR
CUY
CVF
ECM
EIF
NPM
AAYXX
CITATION
3V.
7XB
88I
8FE
8FH
8FK
ABUWG
AFKRA
AZQEC
BBNVY
BENPR
BHPHI
CCPQU
DWQXO
GNUQQ
HCIFZ
LK8
M2P
M7P
PIMPY
PQEST
PQQKQ
PQUKI
PRINS
Q9U
7X8
5PM
DOA
DOI 10.7717/peerj.14938
DatabaseName Medline
MEDLINE
MEDLINE (Ovid)
MEDLINE
MEDLINE
PubMed
CrossRef
ProQuest Central (Corporate)
ProQuest Central (purchase pre-March 2016)
Science Database (Alumni Edition)
ProQuest SciTech Collection
ProQuest Natural Science Collection
ProQuest Central (Alumni) (purchase pre-March 2016)
ProQuest Central (Alumni)
ProQuest Central
ProQuest Central Essentials
Biological Science Collection
ProQuest Central
Natural Science Collection
ProQuest One Community College
ProQuest Central Korea
ProQuest Central Student
SciTech Premium Collection
Biological Sciences
ProQuest Science Database
Biological Science Database
Publicly Available Content Database
ProQuest One Academic Eastern Edition (DO NOT USE)
ProQuest One Academic
ProQuest One Academic UKI Edition
ProQuest Central China
ProQuest Central Basic
MEDLINE - Academic
PubMed Central (Full Participant titles)
Directory of Open Access Journals
DatabaseTitle MEDLINE
Medline Complete
MEDLINE with Full Text
PubMed
MEDLINE (Ovid)
CrossRef
Publicly Available Content Database
ProQuest Science Journals (Alumni Edition)
ProQuest Central Student
ProQuest Biological Science Collection
ProQuest Central Basic
ProQuest Central Essentials
ProQuest Science Journals
ProQuest One Academic Eastern Edition
ProQuest Central (Alumni Edition)
SciTech Premium Collection
ProQuest One Community College
ProQuest Natural Science Collection
Biological Science Database
ProQuest SciTech Collection
ProQuest Central China
ProQuest Central
ProQuest One Academic UKI Edition
Natural Science Collection
ProQuest Central Korea
Biological Science Collection
ProQuest One Academic
ProQuest Central (Alumni)
MEDLINE - Academic
DatabaseTitleList
Publicly Available Content Database

MEDLINE
CrossRef

MEDLINE - Academic
Database_xml – sequence: 1
  dbid: DOA
  name: Open Access: DOAJ - Directory of Open Access Journals
  url: https://www.doaj.org/
  sourceTypes: Open Website
– sequence: 2
  dbid: NPM
  name: PubMed
  url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed
  sourceTypes: Index Database
– sequence: 3
  dbid: EIF
  name: MEDLINE
  url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search
  sourceTypes: Index Database
– sequence: 4
  dbid: BENPR
  name: AUTh Library subscriptions: ProQuest Central
  url: https://www.proquest.com/central
  sourceTypes: Aggregation Database
DeliveryMethod fulltext_linktorsrc
Discipline Medicine
EISSN 2167-8359
EndPage e14938
ExternalDocumentID oai_doaj_org_article_32292803c5c048b9b6a5bfe1a9cd508d
A739839920
10_7717_peerj_14938
36908820
Genre Research Support, Non-U.S. Gov't
Journal Article
GeographicLocations China
Beijing China
GeographicLocations_xml – name: China
– name: Beijing China
GrantInformation_xml – fundername: Liaoning Province College Students Innovation and Entrepreneurship Training Program
  grantid: 2021-114
– fundername: 13th 5-year National Key Research and Development Plan Project
  grantid: 2017YFD0600600
– fundername: Scientific Research Project of Liaoning Education Department
  grantid: LSNZD201905
– fundername: Key Project of Liaoning Natural Science Foundation of China
  grantid: 20170540801
GroupedDBID 3V.
53G
5VS
88I
8FE
8FH
AAFWJ
ABUWG
ADBBV
ADRAZ
AENEX
AFKRA
AFPKN
ALMA_UNASSIGNED_HOLDINGS
AOIJS
AZQEC
BAWUL
BBNVY
BCNDV
BENPR
BHPHI
BPHCQ
CCPQU
CGR
CUY
CVF
DIK
DWQXO
ECGQY
ECM
EIF
GNUQQ
GROUPED_DOAJ
GX1
H13
HCIFZ
HYE
IAO
IEA
IHR
IHW
ITC
KQ8
LK8
M2P
M48
M7P
M~E
NPM
OK1
PIMPY
PQQKQ
PROAC
RPM
W2D
YAO
AAYXX
CITATION
7XB
8FK
PQEST
PQUKI
PRINS
Q9U
7X8
5PM
ID FETCH-LOGICAL-c574t-ee2eac6c35e924bb7f6afcba54b1906628bf91443c43254b571ff6690db005e73
IEDL.DBID RPM
ISSN 2167-8359
IngestDate Tue Oct 22 15:14:24 EDT 2024
Tue Apr 09 21:57:23 EDT 2024
Thu Apr 11 20:02:44 EDT 2024
Thu Oct 10 16:29:24 EDT 2024
Thu Feb 22 23:55:44 EST 2024
Fri Feb 02 04:27:32 EST 2024
Tue Aug 20 22:12:35 EDT 2024
Fri Dec 06 02:51:16 EST 2024
Sat Sep 28 08:16:07 EDT 2024
IsDoiOpenAccess true
IsOpenAccess true
IsPeerReviewed true
IsScholarly true
Keywords Juglans mandshurica Maxim
JmLFY gene
Functional verification
Language English
License 2023 Cai et al.
https://creativecommons.org/licenses/by/4.0
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
LinkModel DirectLink
MergedId FETCHMERGED-LOGICAL-c574t-ee2eac6c35e924bb7f6afcba54b1906628bf91443c43254b571ff6690db005e73
Notes ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ORCID 0000-0001-5496-5009
OpenAccessLink https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10000305/
PMID 36908820
PQID 2784104040
PQPubID 2045935
ParticipantIDs doaj_primary_oai_doaj_org_article_32292803c5c048b9b6a5bfe1a9cd508d
pubmedcentral_primary_oai_pubmedcentral_nih_gov_10000305
proquest_miscellaneous_2786510571
proquest_journals_2784104040
gale_infotracmisc_A739839920
gale_infotracacademiconefile_A739839920
gale_healthsolutions_A739839920
crossref_primary_10_7717_peerj_14938
pubmed_primary_36908820
PublicationCentury 2000
PublicationDate 2023-03-07
PublicationDateYYYYMMDD 2023-03-07
PublicationDate_xml – month: 03
  year: 2023
  text: 2023-03-07
  day: 07
PublicationDecade 2020
PublicationPlace United States
PublicationPlace_xml – name: United States
– name: San Diego
– name: San Diego, USA
PublicationTitle PeerJ (San Francisco, CA)
PublicationTitleAlternate PeerJ
PublicationYear 2023
Publisher PeerJ. Ltd
PeerJ, Inc
PeerJ Inc
Publisher_xml – name: PeerJ. Ltd
– name: PeerJ, Inc
– name: PeerJ Inc
References Hassankhah (10.7717/peerj.14938/ref-9) 2018; 46
Vazquez (10.7717/peerj.14938/ref-31) 2007; 4
Kalve (10.7717/peerj.14938/ref-19) 2020; 226
He (10.7717/peerj.14938/ref-16) 2000; 9
Mori (10.7717/peerj.14938/ref-24) 2017; 7
Hassankhah (10.7717/peerj.14938/ref-10) 2020; 20
Zeng (10.7717/peerj.14938/ref-34) 2018; 19
Dong (10.7717/peerj.14938/ref-5) 2021; 8
He (10.7717/peerj.14938/ref-15) 2017; 56
Ahearn (10.7717/peerj.14938/ref-1) 2001; 10
He (10.7717/peerj.14938/ref-14) 2011; 5
Weigel (10.7717/peerj.14938/ref-32) 1993; 69
Zhao (10.7717/peerj.14938/ref-38) 2020; 39
Dornelas (10.7717/peerj.14938/ref-6) 2006; 223
Fallah (10.7717/peerj.14938/ref-7) 2022; 295
Martina (10.7717/peerj.14938/ref-23) 2015; 32
Bosl (10.7717/peerj.14938/ref-4) 2004; 16
Zhang (10.7717/peerj.14938/ref-37) 2008; 67
Pineiro (10.7717/peerj.14938/ref-26) 1998; 117
Ma (10.7717/peerj.14938/ref-22) 2020; 261
He (10.7717/peerj.14938/ref-13) 2018; 45
Shao (10.7717/peerj.14938/ref-29) 1999; 41
Blazquez (10.7717/peerj.14938/ref-3) 1997; 124
Peña (10.7717/peerj.14938/ref-25) 2001; 19
Tang (10.7717/peerj.14938/ref-30) 2016; 6
Lu (10.7717/peerj.14938/ref-20) 2020; 71
Ma (10.7717/peerj.14938/ref-21) 2019; 261
Alvarez-Buylla (10.7717/peerj.14938/ref-2) 2006; 57
Feng (10.7717/peerj.14938/ref-8) 2016; 36
Weigel (10.7717/peerj.14938/ref-33) 1995; 377
Zou (10.7717/peerj.14938/ref-39) 2014; 34
Jian (10.7717/peerj.14938/ref-17) 2019; 20
Saquib (10.7717/peerj.14938/ref-28) 2020; 11
Zhang (10.7717/peerj.14938/ref-36) 2019; 50
Haughn (10.7717/peerj.14938/ref-12) 1991; 3
Hassankhah (10.7717/peerj.14938/ref-11) 2017; 4
Jin (10.7717/peerj.14938/ref-18) 2019; 17
Roy (10.7717/peerj.14938/ref-27) 2009; 49
Zhang (10.7717/peerj.14938/ref-35) 2003; 5
References_xml – volume: 57
  start-page: 3099
  issue: 12
  year: 2006
  ident: 10.7717/peerj.14938/ref-2
  article-title: Unique and redundant functional domains of APETALA1 and CAULI FLOWER, two recently duplicated Arabidopsis thaliana Xoral MADS-genes
  publication-title: Journal of Experimental Botany
  doi: 10.1093/jxb/erl081
  contributor:
    fullname: Alvarez-Buylla
– volume: 295
  start-page: 110885
  year: 2022
  ident: 10.7717/peerj.14938/ref-7
  article-title: Breeding of Persian walnut: aiming to introduce late-leafing and early-harvesting varieties by targeted hybridization
  publication-title: Scientia Horticulturae
  doi: 10.1016/j.scienta.2022.110885
  contributor:
    fullname: Fallah
– volume: 17
  start-page: 396
  issue: 2
  year: 2019
  ident: 10.7717/peerj.14938/ref-18
  article-title: Cloning and structural analysis of the LEAFY homologous gene of A
  publication-title: Molecular Plant Breeding
  doi: 10.13271/j.mpb.017.000396
  contributor:
    fullname: Jin
– volume: 11
  start-page: 2
  issue: 3
  year: 2020
  ident: 10.7717/peerj.14938/ref-28
  article-title: The critical role of miRNAs in regulation of flowering time and flower development
  publication-title: Genes
  doi: 10.3390/genes11030319
  contributor:
    fullname: Saquib
– volume: 124
  start-page: 3835
  issue: 19
  year: 1997
  ident: 10.7717/peerj.14938/ref-3
  article-title: LEAFY expression and flower initiation in Arabidopsis
  publication-title: Development
  doi: 10.1007/s004290050103
  contributor:
    fullname: Blazquez
– volume: 7
  start-page: 41124
  year: 2017
  ident: 10.7717/peerj.14938/ref-24
  article-title: Identification of RAN1 orthologue associated with sex determination through whole genome sequencing analysis in fig (Ficus carica L.)
  publication-title: Scientific Reports
  doi: 10.1038/srep41124
  contributor:
    fullname: Mori
– volume: 117
  start-page: 1
  issue: 1
  year: 1998
  ident: 10.7717/peerj.14938/ref-26
  article-title: The control of flowering time and floral identity in Arabidopsis
  publication-title: Plant Physiology
  doi: 10.1104/pp.117.1.1
  contributor:
    fullname: Pineiro
– volume: 20
  start-page: 1
  issue: 1
  year: 2020
  ident: 10.7717/peerj.14938/ref-10
  article-title: Flowering in Persian walnut: patterns of gene expression during flower development
  publication-title: BMC Plant Biology
  doi: 10.1186/s12870-020-02372-w
  contributor:
    fullname: Hassankhah
– volume: 4
  start-page: 259
  issue: 2
  year: 2017
  ident: 10.7717/peerj.14938/ref-11
  article-title: Persian walnut phenology: effect of chilling and heat requirements on budbreak and flowering date
  publication-title: International Journal of Horticultural Science and Technology
  doi: 10.22059/IJHST.2018.260944.249
  contributor:
    fullname: Hassankhah
– volume: 34
  start-page: 56
  year: 2014
  ident: 10.7717/peerj.14938/ref-39
  article-title: Genomic identification, classification and evolution analysis of Lhca gene family in four species of Euphorbiaceae
  publication-title: Journal of Central South University of Forestry and Technology
  contributor:
    fullname: Zou
– volume: 50
  start-page: 280
  issue: 3
  year: 2019
  ident: 10.7717/peerj.14938/ref-36
  article-title: Study on changes of material metabolism during flower bud formation of Juglans mandshurica
  publication-title: Journal of Shenyang Agricultural University
  contributor:
    fullname: Zhang
– volume: 261
  start-page: 109014
  year: 2019
  ident: 10.7717/peerj.14938/ref-21
  article-title: Cloning and characterization of a homologue of the FLORICAULA/LEAFY gene in Ficus carica L. FcLFY, and its role in flower bud differentiation
  publication-title: Scientia Horticulturae
  doi: 10.1016/j.scienta.2019.109014
  contributor:
    fullname: Ma
– volume: 19
  start-page: 2
  issue: 1
  year: 2018
  ident: 10.7717/peerj.14938/ref-34
  article-title: Soybean MADS-box gene GmAGL1 promotes flowering via the photoperiod pathway
  publication-title: BMC Genomics
  doi: 10.1186/s12864-017-4402-2
  contributor:
    fullname: Zeng
– volume: 41
  start-page: 42
  issue: 3
  year: 1999
  ident: 10.7717/peerj.14938/ref-29
  article-title: Cloning of Arabidopsis thaliana LFY cDNA and its transformation into Crysanthemum
  publication-title: Acta Botany
  doi: 10.1007/BF00041177
  contributor:
    fullname: Shao
– volume: 10
  start-page: 1130
  issue: 10
  year: 2001
  ident: 10.7717/peerj.14938/ref-1
  article-title: NFL1, a Nicotiana tabacum LEAFY-like gene, controls meristem initiation and floral structure
  publication-title: Plant and Cell Physiology
  doi: 10.1093/pcp/pce143
  contributor:
    fullname: Ahearn
– volume: 20
  start-page: 4
  issue: 1
  year: 2019
  ident: 10.7717/peerj.14938/ref-17
  article-title: Joint QTL mapping and transcriptome sequencing analysis reveal candidate flowering time genes in Brassica napus L
  publication-title: BMC Genomics
  doi: 10.1186/s12864-018-5356-8
  contributor:
    fullname: Jian
– volume: 36
  start-page: 25
  issue: 2
  year: 2016
  ident: 10.7717/peerj.14938/ref-8
  article-title: Phylogenetic evolution and research progress of LEAFY homologous genes
  publication-title: Journal of Central South University of Forestry and Technology
  contributor:
    fullname: Feng
– volume: 261
  start-page: 109014
  year: 2020
  ident: 10.7717/peerj.14938/ref-22
  article-title: Cloning and characterization of a homologue of the FLORICAULA/LEAFY gene in Ficus carica L., FcLFY, and its role in flower bud differentiation
  publication-title: Scientia Horticulturae
  doi: 10.1016/j.scienta.2019.109014
  contributor:
    fullname: Ma
– volume: 4
  start-page: 46
  issue: 5
  year: 2007
  ident: 10.7717/peerj.14938/ref-31
  article-title: Characterization of the expression patterns of LEAFY/FLORICAULA and NEEDLY orthologs in female and male cones of the conifer genera Picea, Podocarpus, and Taxus: implications for current evo-devo hypotheses for gymnosperms
  publication-title: Evolution and Development
  doi: 10.1111/j.1525-142X.2007.00182.x
  contributor:
    fullname: Vazquez
– volume: 69
  start-page: 843
  issue: 5
  year: 1993
  ident: 10.7717/peerj.14938/ref-32
  article-title: LEAFY controls floral meristem identity in Arabidopsis
  publication-title: Cell
  doi: 10.1007/978-1-4757-9607-0_17
  contributor:
    fullname: Weigel
– volume: 39
  start-page: 294
  issue: 3
  year: 2020
  ident: 10.7717/peerj.14938/ref-38
  article-title: Expression and function analysis of Arabidopsis transcription factor LFY2
  publication-title: Journal of Electron Microscopy
  contributor:
    fullname: Zhao
– volume: 3
  start-page: 771
  issue: 8
  year: 1991
  ident: 10.7717/peerj.14938/ref-12
  article-title: LEAFY, a homeotic gene that regulates inflorescence development in Arabidopsis
  publication-title: The Plant Cell
  doi: 10.1105/tpc.3.8.771
  contributor:
    fullname: Haughn
– volume: 32
  start-page: 121
  year: 2015
  ident: 10.7717/peerj.14938/ref-23
  article-title: Flowering time regulation in crops-what did we learn from Arabidopsis
  publication-title: Current Opinion in Biotechnology
  doi: 10.1016/j.copbio.2014.11.023
  contributor:
    fullname: Martina
– volume: 45
  start-page: 1479
  issue: 8
  year: 2018
  ident: 10.7717/peerj.14938/ref-13
  article-title: Screening and expression analysis of related genes based on transcriptome sequencing of ginkgo flower buds at three differentiation stages
  publication-title: Acta Horticulturae Sinica
  doi: 10.16420/j.issn.0513-353x.2018-0023
  contributor:
    fullname: He
– volume: 5
  start-page: 589
  year: 2003
  ident: 10.7717/peerj.14938/ref-35
  article-title: Research progress on molecular mechanism of plant flower development
  publication-title: Bulletin of Botany
  contributor:
    fullname: Zhang
– volume: 67
  start-page: 419
  issue: 4
  year: 2008
  ident: 10.7717/peerj.14938/ref-37
  article-title: Overexpression of the cucumber LEAFY homolog CFL and hormone treatments alter flower development in gloxinia (Sinningia speciosa)
  publication-title: Plant Molecular Biology
  doi: 10.1007/s11103-008-9330-8
  contributor:
    fullname: Zhang
– volume: 8
  start-page: 115
  issue: 1
  year: 2021
  ident: 10.7717/peerj.14938/ref-5
  article-title: Auxin-induced AUXIN RESPONSE FACTOR4 activates APETALA1 and FRUITFULL to promote flowering in woodland strawberry
  publication-title: Horticulture Research
  doi: 10.1038/s41438-021-00550-x
  contributor:
    fullname: Dong
– volume: 46
  start-page: 700
  issue: 2
  year: 2018
  ident: 10.7717/peerj.14938/ref-9
  article-title: Flower development in walnut: altering the flowering pattern by gibberellic acid application
  publication-title: Notulae Botanicae Horti Agrobotanici Cluj-Napoca
  doi: 10.15835/nbha46211183
  contributor:
    fullname: Hassankhah
– volume: 16
  start-page: 2601
  issue: 10
  year: 2004
  ident: 10.7717/peerj.14938/ref-4
  article-title: Divergent roles of a pair of homologous Jumonji/Zinc-finger-class transcription factor proteins in the regulation of Arabidopsis flowering time
  publication-title: The Plant Cell
  doi: 10.1105/tpc.104.025353
  contributor:
    fullname: Bosl
– volume: 19
  start-page: 263
  issue: 3
  year: 2001
  ident: 10.7717/peerj.14938/ref-25
  article-title: Constitutive expressing of Arabidopsis LEAFY or APETALA genes in citrus reduces their generation time
  publication-title: Nature Biotechnology
  doi: 10.1038/85719
  contributor:
    fullname: Peña
– volume: 223
  start-page: 306
  year: 2006
  ident: 10.7717/peerj.14938/ref-6
  article-title: The tropical cedar tree (Cedrela fissilis Vell., Meliaceae) homolog of the Arabidopsis LEAFY gene is expressed in reproductive tis-sues and can complement Arabidopsis leafy mutants
  publication-title: Planta
  doi: 10.1007/s00425-005-0086-y
  contributor:
    fullname: Dornelas
– volume: 49
  start-page: 930
  issue: 3
  year: 2009
  ident: 10.7717/peerj.14938/ref-27
  article-title: Overexpression of AtLEAFY accelerates flowering in Brassica juncea
  publication-title: Crop Science
  doi: 10.2135/cropsci2008.03.0118
  contributor:
    fullname: Roy
– volume: 377
  start-page: 495
  issue: 6549
  year: 1995
  ident: 10.7717/peerj.14938/ref-33
  article-title: A developmental switch sufficient for flower initiation in diverse plants
  publication-title: Nature
  doi: 10.1590/S1516-05722009000400015
  contributor:
    fullname: Weigel
– volume: 5
  start-page: 366
  issue: 3
  year: 2011
  ident: 10.7717/peerj.14938/ref-14
  article-title: Molecular cloning and sequence analysis of an LFY homologous gene from Juglans regia L
  publication-title: Frontiers of Agriculture in China
  doi: 10.1007/s11703-011-1095-1
  contributor:
    fullname: He
– volume: 56
  start-page: 4
  issue: 8
  year: 2017
  ident: 10.7717/peerj.14938/ref-15
  article-title: Construction of walnut JrLFY gene expression vector and screening of engineered bacteria
  publication-title: Hubei Agricultural Science
  contributor:
    fullname: He
– volume: 9
  start-page: 223
  issue: 3
  year: 2000
  ident: 10.7717/peerj.14938/ref-16
  article-title: Transformation of rice with the Arabidopsis floral regulator LEAFY causes early heading
  publication-title: Transgenic Research
  doi: 10.1023/a:1008992719010
  contributor:
    fullname: He
– volume: 71
  start-page: 4057
  issue: 14
  year: 2020
  ident: 10.7717/peerj.14938/ref-20
  article-title: Alternate expression of CONSTANS-LIKE 4 in short days and CONSTANS in long days facilitates day-neutral response in Rosa chinensis
  publication-title: Journal of Experimental Botany
  doi: 10.1093/jxb/eraa161
  contributor:
    fullname: Lu
– volume: 6
  start-page: 37306
  year: 2016
  ident: 10.7717/peerj.14938/ref-30
  article-title: An ortholog of LEAFY in Jatropha curcas regulates flowering time and floral organ development
  publication-title: Scientific Reports
  doi: 10.1038/srep37306
  contributor:
    fullname: Tang
– volume: 226
  start-page: 1766
  issue: 6
  year: 2020
  ident: 10.7717/peerj.14938/ref-19
  article-title: Osmotic stress inhibits leaf growth of Arabidopsis thaliana by enhancing ARF-mediated auxin responses
  publication-title: New Phytologist
  doi: 10.1111/nph.16490
  contributor:
    fullname: Kalve
SSID ssj0000826083
Score 2.33983
Snippet In this study, a pBI121- plant expression vector was constructed on the basis of obtaining the full-length sequence of the gene from , which was then used for...
In this study, a pBI121- JmLFY plant expression vector was constructed on the basis of obtaining the full-length sequence of the JmLFY gene from Juglans...
In this study, a pBI121-JmLFY plant expression vector was constructed on the basis of obtaining the full-length sequence of the JmLFY gene from Juglans...
SourceID doaj
pubmedcentral
proquest
gale
crossref
pubmed
SourceType Open Website
Open Access Repository
Aggregation Database
Index Database
StartPage e14938
SubjectTerms Agricultural Science
Arabidopsis - genetics
Arabidopsis thaliana
Children
Cloning
Flowering
Flowers & plants
Flowers - genetics
Functional verification
Gene expression
Genetic aspects
Genetic engineering
Genetic research
Genetic transformation
Genetically modified plants
Health aspects
Infections
Inflorescence - genetics
JmLFY gene
Juglans
Juglans mandshurica
Juglans mandshurica Maxim
Leaves
Molecular Biology
Mutants
Plant Breeding
Plant Science
Seeds
Transgenic plants
SummonAdditionalLinks – databaseName: Directory of Open Access Journals
  dbid: DOA
  link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV1LS8QwEA6yB_Eivl2fEQRPZdumSdqjiossricFPYUkTdgFt7voCv58Z9K6tHjw4rUzLe288k0zMyHkEts9U-9FlOqcRZmJXWRKC8lKyRKLgJvF2Cg8fhT3z9nohb-0jvrCmrB6PHAtuAEYXIEnKFluwdhMYYTmxrtEF7YEcFGG6BunrWQqxGBAzQAu6oY8CSnLYOHcO8aFAjtRWktQmNT_Ox63FqRusWRr9Rlukc0GNtLr-nW3yZqrdsj6uNkY3yXlENan-rceBePE-p8gcjr3FCAeHc0ehq8UjMVR3SjElRR_wgayf8PD0mAVQ_4RQurqg86wC3iCU4c0Heuv6WyPPA_vnm7vo-YAhchymS0j51KIq8Iy7iDNMkZ6ob01mmcGcIAQaW58ARkVsxmDRNFwmYDaIF8u0TmdZPukV80rd0iojGPrE1t4HLhXJDa3wkCqw1OumYx13CeXPzJVi3pOhoL8AkWvguhVEH2f3KC8Vyw43DpcAJWrRuXqL5X3yTlqS9WdoisXVdeSFTkO2oW3uQoc6KSgNKubXgP4Fhx31eE86XCCc9ku-cciVOPcHyrs1ULwy4B8sSLjnViwVrn5Z-ARiF1l0icHtQGtPpoJLC7Dh-cd0-pIpUupppMw-jvsxkCIPvoPOR6TjRQgW6iokyekt3z_dKcAsZbmLHjTN0SrJLs
  priority: 102
  providerName: Directory of Open Access Journals
– databaseName: ProQuest Central
  dbid: BENPR
  link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfR1da9swUGwpjL2Mfc9r12lQ2JOpbVmS_TTa0VDCUsZYoXsSkiy1hcXOkhT283snK17MoK_ROdj3faf7IOQI2z0L70Va6IqlpclcahoLwUrDcosON8uwUXh-Ic4vy9kVv4oJt3Usq9zqxKCom85ijvw4XJABx5XZl-WfFLdG4e1qXKHxmOwVOauqCdk7Pbv4_mPIsoCBE-Bk9I15EkKX46VzK9QPNXak7JiiMLH_f728Y5jGRZM7Vmj6nDyL7iM96en9gjxy7UvyZB4vyF-RZgp2qk_vUWBSrAMKqKedp-Dq0dni2_QXBaZxVEfCuIZiMjYc-9-4NA2sGcLP0LVu13SB3cA3OH1I07n-e7t4TS6nZz-_nqdxkUJquSw3qXMF6FdhGXcQbhkjvdDeGs1LA_6AEEVlfA2RFbMlg4DRcJkD-SBublBInWRvyKTtWveOUJll1ue29jh4r85tZYWBkIcXXDOZ6SwhR1ucqmU_L0NBnIGoVwH1KqA-IaeI7wEEh1yHH7rVtYoyo0DX1Lg8y3ILesbURmhuvMt1bRvwK5uEfERqqb5jdBBVdSJZXeHAXXibzwEChRWIZnXsOYBvwbFXI8iDESQImR0fbzlCRSFfq38smZBPwzE-iYVrrevuAoxAH1bmCXnbM9Dw0UxgkRn-eTVirRFWxift7U0YAR5uZUBVv3_4vfbJ0wKcslAzJw_IZLO6cx_AidqYwygp9xkUHYM
  priority: 102
  providerName: ProQuest
– databaseName: Scholars Portal Journals: Open Access
  dbid: M48
  link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV3dT9swED8xJk17QRtso-PLk5B4Ckvi2E6eJpioULXuiUrwZNmOPZBoCqVI7L_nzkkrsu1pr_U5au7zd_HdGeCQ2j3zEGSSm5InhU19YmuHyUrNM0eAm6fUKDz-Kc8nxehSXK7B8jLOjoEP_0zt6D6pyfz2-On-9zc0eMSvxwqzka933s_J5CtevoLXOYZEqu0adzg_umQE0Yg12v68P_f0IlIc3P-3e34Rn_q1ky-C0fAdbHQokp20Yn8Pa77ZhDfj7px8C-ohhqv2Kx9DXaVyoCgBNgsMER8bTX8Mrxjqjmemk4-vGX2Tjcvhlu5Ow6BG9CNC2M0Dm1JT8DUNITJsbJ5uph9gMjy7-H6edPcpJE6oYpF4n6OblY4Lj1mXtSpIE5w1orAIC6TMSxsqTLC4KzjmjVaoDKWI6XNNtuoV_wjrzazx28BUmrqQuSrQ_L0qc6WTFjMfkQvDVWrSARwuearv2rEZGtMNYr2OrNeR9QM4JX6vSGjWdfxhNv-lO9PR6HIqukPLCYfuxlZWGmGDz0zlaoSX9QAOSFq6bRxdWaw-Ubwqae4u_pujSEFahEJzpms9wHeh6Vc9yt0eJdqa6y8vNUIvVVXHo1v0hQUuf1kt006qX2v87DHSSIKyKhvAp1aBVi_NJdWa0cPLnmr1uNJfaW6u4yTweDiDHvvz_2_dgbc54rZYVqd2YX0xf_R7iLMWdj_a0DPY5Sqg
  priority: 102
  providerName: Scholars Portal
Title Functional verification of the JmLFY gene associated with the flowering of Juglans mandshurica Maxim
URI https://www.ncbi.nlm.nih.gov/pubmed/36908820
https://www.proquest.com/docview/2784104040
https://search.proquest.com/docview/2786510571
https://pubmed.ncbi.nlm.nih.gov/PMC10000305
https://doaj.org/article/32292803c5c048b9b6a5bfe1a9cd508d
Volume 11
hasFullText 1
inHoldings 1
isFullTextHit
isPrint
link http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV3da9swEBf9gLGXsXVf2bpUg8Ke3NiWJdmPbWlawlzKWCF7EpIsrYHaCWkK-_N3J9shZm978UN0DvZ9_k6-OxFyiu2eqfciSnXOoszELjKVhWSlYolFwM1ibBQub8XNfTab8_keEX0vTCjat2Zx1jzWZ83iIdRWrmo76evEJnflZdiTBkWd7JN9iL87OXrwv4CYAVi0zXgS0pXJyrk1-oQCu1B2wk-Y0v-vL94JRsNCyZ3IM31NXnWQkZ63j_aG7LnmiLwou4_ib0k1hdjUbulRUEys_QnspktPAd7RWf19-ouCojiqO2G4iuIGbFj2j3hQGkQwpJ8hnG6eaI0dwA84cUjTUv9Z1O_I_fTq5-VN1B2eEFkus03kXAo-VVjGHaRYxkgvtLdG88wABhAizY0vIJtiNmOQJBouExAZ5MoVGqaT7D05aJaN-0iojGPrE1t4HLZXJDa3wkCaw1OumYx1PCKnPU_Vqp2RoSC3QNarwHoVWD8iF8jvLQkOtg4_LNe_VSdeBf6lwAOzLLfgW0xhhObGu0QXtgIsWY3ICUpLtV2iW_NU55IVOQ7Zhaf5FijQQEFoVnd9BvAuOOpqQHk8oATDssPlXiNUZ9hPKnynBceXwfLX7TLeicVqjVs-BxqBuFUmI_KhVaDtSzOBhWX45_lAtQZcGa6AFYSx373Wf_r_Wz-TlymAtFBDJ4_JwWb97L4AqNqYMTm8uLq9-zEOmxJwvZ4ncC2zfBys6y8pFCgH
link.rule.ids 230,314,727,780,784,864,885,2102,21388,24318,27924,27925,33744,33745,43805,53791,53793,74302
linkProvider National Library of Medicine
linkToHtml http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfR1db9Mw0IJOAl4Q3wQGM9IknqLly3byhDa0qpS2QmiTxpNlO_Y2iSal7SR-PneOGxoh8VpfquS-73wfhBxju2fmHI8zVeZxoRMb69pAsFLnqUGHO0-wUXi-4JPLYnrFrkLCbRPKKnc60SvqujWYIz_xF2TAcUXyafUrxq1ReLsaVmjcJwc4OZ2NyMHZ-eLb9z7LAgaOg5PRNeYJCF1OVtauUT9U2JGyZ4r8xP5_9fKeYRoWTe5ZofET8ji4j_S0o_dTcs82z8iDebggf07qMdipLr1HgUmxDsijnraOgqtHp8vZ-AcFprFUBcLYmmIy1h-7n7g0DawZwk_RtW42dIndwDc4fUjRufp9u3xBLsfnF58ncVikEBsmim1sbQb6lZucWQi3tBaOK2e0YoUGf4DzrNSugsgqN0UOAaNmIgXyQdxco5Bakb8ko6Zt7GtCRZIYl5rK4eC9KjWl4RpCHpYxlYtEJRE53uFUrrp5GRLiDES99KiXHvUROUN89yA45Nr_0K6vZZAZCbqmwuVZhhnQM7rSXDHtbKoqU4NfWUfkCKklu47RXlTlqcirEgfuwtt89BAorEA0o0LPAXwLjr0aQB4OIEHIzPB4xxEyCPlG_mXJiHzoj_FJLFxrbHvnYTj6sCKNyKuOgfqPzjkWmeGflwPWGmBleNLc3vgR4P5WBlT1m_-_1xF5OLmYz-Tsy-LrW_IoAwfN18-JQzLaru_sO3Cotvp9kJo_lBcgaw
linkToPdf http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV3da9swEBdbCmUvY99z160aFPZkYlu2ZD-NdmvosiaUsUL3JCRZWguLnSYp7M_fnax4MYO9Rudg3-fvpLsTIcfY7pk5x-NMlSzOdWJjXRtIVmqWGgTcLMFG4dmcn1_l0-viOtQ_rUNZ5dYnekddtwb3yMf-gAw0Lk_GLpRFXH6efFzexXiDFJ60hus0HpI9iIpJNiJ7p2fzy2_9jgsEOw6Ao2vSE5DGjJfWrtBXVNidshOW_PT-f330TpAaFlDuRKTJE_I4QEl60sn-KXlgm2dkfxYOy5-TegIxq9vqo6CwWBPkxUBbRwH20eniYvKDggJZqoKQbE1xY9Yvu194gRpENqSfIsxu1nSBncE3OIlI0Zn6fbt4Qa4mZ98_ncfhUoXYFCLfxNZm4Gu5YYWF1Etr4bhyRqsi14ANOM9K7SrIspjJGSSPuhApiBJy6BoN1gr2koyatrGvCRVJYlxqKodD-KrUlIZrSH-KrFBMJCqJyPGWp3LZzc6QkHMg66VnvfSsj8gp8rsnwYHX_od29VMG-5Hgdyq8SMsUBnyOrjRXhXY2VZWpAWPWETlCacmue7Q3W3kiWFXi8F14mw-eAg0XhGZU6D-Ab8ERWAPKwwElGJwZLm81QgaDX8u_6hmR9_0yPolFbI1t7z0NRzwr0oi86hSo_2jGseAM_7wcqNaAK8OV5vbGjwP3JzTgtg_-_15HZB8MRl58mX99Qx5lgNV8KZ04JKPN6t6-BWy10e-C0fwB_tQkmA
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Functional+verification+of+the+JmLFY+gene+associated+with+the+flowering+of+Juglans+mandshurica+Maxim&rft.jtitle=PeerJ+%28San+Francisco%2C+CA%29&rft.au=Cai%2C+Jiayou&rft.au=Jia%2C+Ruoxue&rft.au=Jiang%2C+Ying&rft.au=Fu%2C+Jingqi&rft.date=2023-03-07&rft.pub=PeerJ+Inc&rft.eissn=2167-8359&rft.volume=11&rft_id=info:doi/10.7717%2Fpeerj.14938&rft.externalDBID=PMC10000305
thumbnail_l http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=2167-8359&client=summon
thumbnail_m http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=2167-8359&client=summon
thumbnail_s http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=2167-8359&client=summon