Electroporation of AsCpf1/RNP at the Zygote Stage is an Efficient Genome Editing Method to Generate Knock-Out Mice Deficient in Leukemia Inhibitory Factor - Kim, Yeon Sun; Kim, Gyeong Ryeong; Park, Mira; Yang, Seung Chel; Park, So Hee; Won, Ji Eun; Lee, Ju Hee; Shin, Ha Eun; Song, Haengseok; Kim, Hye-Ryun (Tissue engineering and regenerative medicine, 2020)
Keywords: Animals; Base Sequence; Blastocyst - metabolism; CRISPR-Associated Protein 9; CRISPR-Cas Systems; Electroporation - methods; Endonucleases; Gene Editing - methods; Gene Targeting; Leukemia Inhibitory Factor - genetics; Leukemia Inhibitory Factor - metabolism; Mice, Knockout; Microinjections; RNA, Guide, CRISPR-Cas Systems - genetics; Zygote - metabolism; LIF; CRISPR/AsCpf1; Electroporation; Embryo; Gene targeting; AsCpf1/RNP