Simultaneous NAD(P)H and FAD fluorescence lifetime microscopy of long UVA–induced metabolic stress in reconstructed human skin

• Published in: Scientific reports Vol. 11; no. 1; pp. 22171 - 15
• Main Authors: Ung, Thi Phuong Lien, Lim, Seongbin, Solinas, Xavier, Mahou, Pierre, Chessel, Anatole, Marionnet, Claire, Bornschlögl, Thomas, Beaurepaire, Emmanuel, Bernerd, Françoise, Pena, Ana-Maria, Stringari, Chiara
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 12.11.2021; Nature Publishing Group; Nature Portfolio
• Subjects: 631/443/319; 639/766/400; 639/766/400/385; 639/766/930/328; Adenine; Biomarkers; Cellular Biology; Deep Learning; Dermatology; Fibroblasts; Flavin; Flavin-adenine dinucleotide; Flavin-Adenine Dinucleotide - metabolism; Fluorescence; Fluorescent Antibody Technique; Glycolysis; Human health and pathology; Humanities and Social Sciences; Humans; Immunohistochemistry; Keratinocytes; Life Sciences; Metabolism; Microscopy; Microscopy, Fluorescence; multidisciplinary; NAD; NADP - metabolism; Optical Imaging; Oxidative phosphorylation; Oxidative stress; Phosphorylation; Reactive oxygen species; Science; Science (multidisciplinary); Skin; Skin - metabolism; Skin - radiation effects; Stress, Physiological - radiation effects; Subcellular Processes; Sunlight; Ultraviolet Rays

Solar ultraviolet longwave UVA1 exposure of human skin has short-term consequences at cellular and molecular level, leading at long-term to photoaging. Following exposure, reactive oxygen species (ROS) are generated, inducing oxidative stress that might impair cellular metabolic activity. However, t...