Performance comparison of one-color and two-color platforms within the Microarray Quality Control (MAQC) project

• Published in: Nature biotechnology Vol. 24; no. 9; pp. 1140 - 1150
• Main Authors: Patterson, Tucker A, Lobenhofer, Edward K, Fulmer-Smentek, Stephanie B, Collins, Patrick J, Chu, Tzu-Ming, Bao, Wenjun, Fang, Hong, Kawasaki, Ernest S, Hager, Janet, Tikhonova, Irina R, Walker, Stephen J, Zhang, Liang, Hurban, Patrick, de Longueville, Francoise, Fuscoe, James C, Tong, Weida, Shi, Leming, Wolfinger, Russell D
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 01.09.2006; Nature Publishing Group
• Subjects: Agriculture; analysis; Bioinformatics; Biomedical Engineering/Biotechnology; Biomedicine; Biotechnology; Color; Comparative studies; Correlation coefficient; Design; Design of experiments; Equipment Design; Equipment Failure Analysis; Gene expression; Gene Expression Profiling - instrumentation; Gene Expression Profiling - methods; In Situ Hybridization, Fluorescence - instrumentation; Life Sciences; Microscopy, Fluorescence, Multiphoton - instrumentation; Microscopy, Fluorescence, Multiphoton - methods; Oligonucleotide Array Sequence Analysis - instrumentation; Quality Assurance, Health Care - methods; Quality Control; Reproducibility of Results; Ribonucleic acid; RNA; Sensitivity and Specificity; Spectrometry, Fluorescence - instrumentation; Spectrometry, Fluorescence - methods; United States; United States

Microarray-based expression profiling experiments typically use either a one-color or a two-color design to measure mRNA abundance. The validity of each approach has been amply demonstrated. Here we provide a simultaneous comparison of results from one- and two-color labeling designs, using two inde...