Fast, three-dimensional super-resolution imaging of live cells

• Published in: Nature methods Vol. 8; no. 6; pp. 499 - 505
• Main Authors: Jones, Sara A, Shim, Sang-Hee, He, Jiang, Zhuang, Xiaowei
• Format: Journal Article
• Language: English
• Published: New York Nature Publishing Group US 01.06.2011; Nature Publishing Group
• Subjects: 631/1647/245/2225; 631/1647/328/2238; 631/80/2373; Animals; Bioinformatics; Biological Microscopy; Biological Techniques; Biomedical and Life Sciences; Biomedical Engineering/Biotechnology; Cell Line; Cellular biology; Clathrin - metabolism; Coated Pits, Cell-Membrane - metabolism; Dyes; Emissions; Endocytosis; Fluorescence; Fluorescence microscopy; Fluorescent Dyes; Genetic aspects; Image Processing, Computer-Assisted - methods; Image Processing, Computer-Assisted - statistics & numerical data; Life Sciences; Microscopy; Microscopy, Fluorescence - methods; Microscopy, Fluorescence - statistics & numerical data; Probes; Proteins; Proteomics; Scientific imaging; Structure; Transferrin - metabolism; Wavelengths; United States

Judicious choice of probes and imaging conditions allows two-dimensional super-resolution imaging of live cells at speeds up to 2 Hz with ~25-nm resolution and three-dimensional super-resolution imaging at ~1 Hz with ~30 nm x-y and ~50 nm z dimension resolution using stochastic optical reconstructio...