Dissecting horizontal and vertical gene transfer of antibiotic resistance plasmid in bacterial community using microfluidics

• Published in: Environment international Vol. 131; p. 105007
• Main Authors: Li, Bing, Qiu, Yong, Song, Yanqing, Lin, Hai, Yin, Huabing
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Ltd 01.10.2019; Elsevier
• Subjects: activated sludge; amoxicillin; Anti-Bacterial Agents - pharmacology; antibiotic resistance; Antibiotic resistance genes; Antibiotics; Bacteria - drug effects; Bacteria - genetics; bacterial communities; Cell tracking; Conjugation, Genetic; Drug Resistance, Bacterial - genetics; Escherichia coli; gene transfer; Gene Transfer, Horizontal; Horizontal gene transfer; Humans; image analysis; Microbiota; microfluidic technology; Microfluidics; plasmids; Plasmids - genetics; Sewage - microbiology; tetracycline; trimethoprim; Vertical gene transfer; Antibiotics; Cell tracking; Antibiotic resistance genes; Microfluidics; Horizontal gene transfer; Vertical gene transfer

The spread of antibiotic resistance genes (ARGs) has become an emerging threat to the global health. Although horizontal gene transfer (HGT) is regarded as one of the major pathways, more evidence has shown the significant involvement of vertical gene transfer (VGT). However, traditional cultivation...