Structure-function relationships in NDP-sugar active SDR enzymes: Fingerprints for functional annotation and enzyme engineering

• Published in: Biotechnology advances Vol. 48; p. 107705
• Main Authors: Da Costa, Matthieu, Gevaert, Ophelia, Van Overtveldt, Stevie, Lange, Joanna, Joosten, Henk-Jan, Desmet, Tom, Beerens, Koen
• Format: Journal Article
• Language: English
• Published: England Elsevier Inc 01.05.2021; Elsevier Science Ltd
• Subjects: Annotations; Biosynthesis; Carbohydrates; Decarboxylase; Dehydratase; Enzymes; Epimerase; Fingerprints; Galactose; Humans; Metabolic disorders; NDP-sugars; Nucleotides; Oxidoreductases; Reductase; Reductases; SDR superfamily; Sequence Alignment; Short-chain dehydrogenase/reductase; Specificity fingerprints; Structure-Activity Relationship; Structure-function relationship; Substrate Specificity; Substrates; Sugar; Sugars; Virulence; NDP-sugars; Decarboxylase; Dehydratase; SDR superfamily; Specificity fingerprints; Structure-function relationship; Epimerase; Reductase; Short-chain dehydrogenase/reductase

Short-chain Dehydrogenase/Reductase enzymes that are active on nucleotide sugars (abbreviated as NS-SDR) are of paramount importance in the biosynthesis of rare sugars and glycosides. Some family members have already been extensively characterized due to their direct implication in metabolic disorde...