Compartmentalized Culture of Perivascular Stroma and Endothelial Cells in a Microfluidic Model of the Human Endometrium

• Published in: Annals of biomedical engineering Vol. 45; no. 7; pp. 1758 - 1769
• Main Authors: Gnecco, Juan S., Pensabene, Virginia, Li, David J., Ding, Tianbing, Hui, Elliot E., Bruner-Tran, Kaylon L., Osteen, Kevin G.
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.07.2017; Springer Nature B.V
• Subjects: Biochemical analysis; Biochemistry; Biological and Medical Physics; Biomedical and Life Sciences; Biomedical Engineering and Bioengineering; Biomedicine; Biophysics; Cell culture; Cell Culture Techniques; Classical Mechanics; Computer simulation; Crosstalk; Cytoskeleton; Decidua; Differentiation; Endometrium; Endometrium - blood supply; Endometrium - cytology; Endometrium - metabolism; Endothelial cells; Female; Fibroblasts; Fluorescence; High resolution; Human Umbilical Vein Endothelial Cells - cytology; Human Umbilical Vein Endothelial Cells - metabolism; Humans; Immunology; Implantation; In vitro methods and tests; Mechanical stimuli; Membranes, Artificial; Menstrual cycle; Microenvironments; Microfluidics; Models, Cardiovascular; Paracrine signalling; Polydimethylsiloxane; Porosity; Prolactin; Reproductive Tissue Engineering; Shear stress; Steroid hormones; Steroids; Stimulation; Stroma; Tissue Engineering - methods; Uterus; Stroma; Organs-on-a-chip; Porous membrane; Endometrium; Microfluidic

The endometrium is the inner lining of the uterus. Following specific cyclic hormonal stimulation, endometrial stromal fibroblasts (stroma) and vascular endothelial cells exhibit morphological and biochemical changes to support embryo implantation and regulate vascular function, respectively. Herein...