Generation of novel Il2rg-knockout mice with clustered regularly interspaced short palindromic repeats (CRISPR) and Cas9

• Published in: Experimental Animals Vol. 69; no. 2; pp. 189 - 198
• Main Authors: Byambaa, Suvd, Uosaki, Hideki, Hara, Hiromasa, Nagao, Yasumitsu, Abe, Tomoyuki, Shibata, Hiroaki, Nureki, Osamu, Ohmori, Tsukasa, Hanazono, Yutaka
• Format: Journal Article
• Language: English
• Published: Japan Japanese Association for Laboratory Animal Science 01.01.2020; Japan Science and Technology Agency
• Subjects: animal model; clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9; CRISPR; Exons; Gene deletion; Genetic disorders; genome-editing; II2rg; Insertion; Interleukin 2; Interleukins; Lymphocytes; Lymphocytes B; Lymphocytes T; mRNA; Mutants; Mutation; Nucleotides; Original; Peripheral blood; Phenotypes; Proteins; Rodents; Severe combined immunodeficiency; X-linked severe combined immunodeficiency (X-SCID); Zygotes; X-linked severe combined immunodeficiency (X-SCID); clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9; II2rg; animal model; genome-editing

X-linked severe combined immunodeficiency (X-SCID) is an inherited genetic disorder. A majority of X-SCID subjects carries point mutations in the Interleukin-2 receptor gamma chain (IL2RG) gene. In contrast, Il2rg-knockout mice recapitulating X-SCID phenotype lack a large part of Il2rg instead of po...