Establishment of a reverse genetics system for SARS-CoV-2 using circular polymerase extension reaction

• Published in: Cell reports (Cambridge) Vol. 35; no. 3; p. 109014
• Main Authors: Torii, Shiho, Ono, Chikako, Suzuki, Rigel, Morioka, Yuhei, Anzai, Itsuki, Fauzyah, Yuzy, Maeda, Yusuke, Kamitani, Wataru, Fukuhara, Takasuke, Matsuura, Yoshiharu
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 20.04.2021; The Authors; Elsevier
• Subjects: Animals; COVID-19 - genetics; CPER; Cricetinae; HEK293 Cells; Humans; infectious clone; mutagenesis; Reverse Genetics; SARS-CoV-2; SARS-CoV-2 - genetics; SARS-CoV-2; reverse genetics; infectious clone; CPER; mutagenesis

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been identified as the causative agent of coronavirus disease 2019 (COVID-19). Although multiple mutations have been observed in SARS-CoV-2, functional analysis of each mutation of SARS-CoV-2 has been limited by the lack of convenient...