Quantitative single-molecule imaging by confocal laser scanning microscopy
A new approach to quantitative single-molecule imaging by confocal laser scanning microscopy (CLSM) is presented. It relies on fluorescence intensity distribution to analyze the molecular occurrence statistics captured by digital imaging and enables direct determination of the number of fluorescent...
Saved in:
Published in | Proceedings of the National Academy of Sciences - PNAS Vol. 105; no. 47; pp. 18176 - 18181 |
---|---|
Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
United States
National Academy of Sciences
25.11.2008
National Acad Sciences |
Subjects | |
Online Access | Get full text |
Cover
Loading…
Abstract | A new approach to quantitative single-molecule imaging by confocal laser scanning microscopy (CLSM) is presented. It relies on fluorescence intensity distribution to analyze the molecular occurrence statistics captured by digital imaging and enables direct determination of the number of fluorescent molecules and their diffusion rates without resorting to temporal or spatial autocorrelation analyses. Digital images of fluorescent molecules were recorded by using fast scanning and avalanche photodiode detectors. In this way the signal-to-background ratio was significantly improved, enabling direct quantitative imaging by CLSM. The potential of the proposed approach is demonstrated by using standard solutions of fluorescent dyes, fluorescently labeled DNA molecules, quantum dots, and the Enhanced Green Fluorescent Protein in solution and in live cells. The method was verified by using fluorescence correlation spectroscopy. The relevance for biological applications, in particular, for live cell imaging, is discussed. |
---|---|
AbstractList | A new approach to quantitative single-molecule imaging by confocal laser scanning microscopy (CLSM) is presented. It relies on fluorescence intensity distribution to analyze the molecular occurrence statistics captured by digital imaging and enables direct determination of the number of fluorescent molecules and their diffusion rates without resorting to temporal or spatial autocorrelation analyses. Digital images of fluorescent molecules were recorded by using fast scanning and avalanche photodiode detectors. In this way the signal-to-background ratio was significantly improved, enabling direct quantitative imaging by CLSM. The potential of the proposed approach is demonstrated by using standard solutions of fluorescent dyes, fluorescently labeled DNA molecules, quantum dots, and the Enhanced Green Fluorescent Protein in solution and in live cells. The method was verified by using fluorescence correlation spectroscopy. The relevance for biological applications, in particular, for live cell imaging, is discussed. A new approach to quantitative single-molecule imaging by confocal laser scanning microscopy (CLSM) is presented. It relies on fluorescence intensity distribution to analyze the molecular occurrence statistics captured by digital imaging and enables direct determination of the number of fluorescent molecules and their diffusion rates without resorting to temporal or spatial autocorrelation analyses. Digital images of fluorescent molecules were recorded by using fast scanning and avalanche photodiode detectors. In this way the signal-to-background ratio was significantly improved, enabling direct quantitative imaging by CLSM. The potential of the proposed approach is demonstrated by using standard solutions of fluorescent dyes, fluorescently labeled DNA molecules, quantum dots, and the Enhanced Green Fluorescent Protein in solution and in live cells. The method was verified by using fluorescence correlation spectroscopy. The relevance for biological applications, in particular, for live cell imaging, is discussed. fluorescence correlation spectroscopy live cells sensitivity A new approach to quantitative single-molecule imaging by confocal laser scanning microscopy (CLSM) is presented. It relies on fluorescence intensity distribution to analyze the molecular occurrence statistics captured by digital imaging and enables direct determination of the number of fluorescent molecules and their diffusion rates without resorting to temporal or spatial autocorrelation analyses. Digital images of fluorescent molecules were recorded by using fast scanning and avalanche photodiode detectors. In this way the signal-to-background ratio was significantly improved, enabling direct quantitative imaging by CLSM. The potential of the proposed approach is demonstrated by using standard solutions of fluorescent dyes, fluorescently labeled DNA molecules, quantum dots, and the Enhanced Green Fluorescent Protein in solution and in live cells. The method was verified by using fluorescence correlation spectroscopy. The relevance for biological applications, in particular, for live cell imaging, is discussed. [PUBLICATION ABSTRACT] |
Author | Ming, Yu Vukojević, Vladana Johansson, Björn Rigler, Rudolf Terenius, Lars Heidkamp, Marcus |
Author_xml | – sequence: 1 fullname: Vukojević, Vladana – sequence: 2 fullname: Heidkamp, Marcus – sequence: 3 fullname: Ming, Yu – sequence: 4 fullname: Johansson, Björn – sequence: 5 fullname: Terenius, Lars – sequence: 6 fullname: Rigler, Rudolf |
BackLink | https://www.ncbi.nlm.nih.gov/pubmed/19011092$$D View this record in MEDLINE/PubMed http://kipublications.ki.se/Default.aspx?queryparsed=id:118018740$$DView record from Swedish Publication Index |
BookMark | eNqFkc1v1DAQxS1URLeFMycg4oDEIa3HH7F9QUJV-VIlhKBny3GcJYvX3sZJ6f73OErUpVw4WLZmfu9pPO8EHYUYHELPAZ8BFvR8F0w6wxIrwjFg_gitACsoK6bwEVphTEQpGWHH6CSlDcZYcYmfoGNQGDJIVujLt9GEoRvM0N26InVh7V25jd7Z0bui25p1LhX1vrAxtNEaX3iTXF8ka0KYWtvO9jHZuNs_RY9b45N7ttyn6PrD5Y-LT-XV14-fL95flZYLOpTESiKYNLStHVRUVqLhjWHGMaNqRWpim5YpQWXdGA5OUEUaIEy0mHAAAfQUqdk3_Xa7sda7Po_Z73U0XX7HRi_1X910dHIaQGKQguGsfTdrM7B1jXVh6I1_aPGgE7qfeh1vNeFSVJRmgzeLQR9vRpcGve2Sdd6b4OKYdKUkJXnRGXz9D7iJYx_yYjTBQKUSkmTofIamHabetfeTANZTwHoKWB8CzoqXf3_gwC-JZuDtAkzKgx3XTGiQICrdjt4P7m7IbPEfNiMvZmSThtjfM4SzijPKcv_V3G9N1Gbdd0lff58-iIFXTOaN_QHYRtA7 |
CitedBy_id | crossref_primary_10_1039_c3pp50404b crossref_primary_10_1096_fj_202101213R crossref_primary_10_1007_s00223_013_9815_6 crossref_primary_10_1186_s40035_021_00244_3 crossref_primary_10_3390_ijms24065794 crossref_primary_10_1039_D1SM01466H crossref_primary_10_3233_JAD_200237 crossref_primary_10_1002_cpcy_39 crossref_primary_10_1021_acs_analchem_9b01813 crossref_primary_10_1051_jbio_2011012 crossref_primary_10_1083_jcb_201809121 crossref_primary_10_3233_JAD_181144 crossref_primary_10_1515_optof_2015_0001 crossref_primary_10_1016_j_isci_2022_105137 crossref_primary_10_1016_j_mod_2015_09_004 crossref_primary_10_3390_molecules27072350 crossref_primary_10_1103_PhysRevE_83_021909 crossref_primary_10_1038_cddis_2015_39 crossref_primary_10_1016_j_yexcr_2014_05_003 crossref_primary_10_1128_MCB_01304_10 crossref_primary_10_1111_j_1742_4658_2009_07107_x crossref_primary_10_1016_j_biomaterials_2010_08_111 crossref_primary_10_1016_j_trac_2023_117085 crossref_primary_10_1007_s11120_017_0346_2 crossref_primary_10_3389_fnagi_2020_608628 crossref_primary_10_1073_pnas_0914595107 crossref_primary_10_3389_fimmu_2016_00016 crossref_primary_10_1364_OE_18_021225 crossref_primary_10_1074_jbc_M113_510883 crossref_primary_10_1101_gad_338061_120 crossref_primary_10_1073_pnas_1018658108 crossref_primary_10_3390_ijms21165742 crossref_primary_10_1117_1_3600002 crossref_primary_10_1002_jemt_22368 crossref_primary_10_1242_dev_168179 crossref_primary_10_1364_OE_504581 crossref_primary_10_1021_la1026416 crossref_primary_10_1016_j_bbrc_2010_04_070 crossref_primary_10_1039_D1CC04799J crossref_primary_10_1007_s00216_012_6373_0 crossref_primary_10_1016_j_bbagrm_2012_02_001 crossref_primary_10_1016_j_bbamem_2017_09_024 crossref_primary_10_1002_ejlt_201800401 crossref_primary_10_1021_ac202313d crossref_primary_10_1002_cbic_201100302 crossref_primary_10_1073_pnas_0914612107 crossref_primary_10_1021_cm5003669 crossref_primary_10_1021_ac1014047 crossref_primary_10_1515_optof_2016_0004 crossref_primary_10_1093_hmg_ddv225 crossref_primary_10_1016_j_snb_2018_04_090 crossref_primary_10_1002_mgg3_666 crossref_primary_10_1371_journal_pone_0213655 crossref_primary_10_1038_s41598_018_30887_8 crossref_primary_10_1039_c3ra46877a crossref_primary_10_1111_tra_12582 crossref_primary_10_1080_13682199_2015_1123343 crossref_primary_10_3233_JAD_215504 crossref_primary_10_1007_s00216_022_04502_9 crossref_primary_10_1017_S0033583514000146 |
Cites_doi | 10.1021/j100036a009 10.1126/science.1127344 10.1103/PhysRevA.10.1938 10.1016/S0006-3495(00)76523-4 10.1117/1.1779234 10.1117/12.146154 10.1038/nmeth922 10.1529/biophysj.105.061788 10.1073/pnas.91.13.5740 10.1016/S0006-3495(94)80801-X 10.1529/biophysj.107.114645 10.1038/nmeth929 10.1016/0301-0104(74)85005-6 10.1016/S0006-3495(99)76912-2 10.2174/138920106777950843 10.1073/pnas.96.24.13756 10.1073/pnas.0606557104 10.1007/s12013-007-9000-5 10.1103/PhysRevLett.29.705 10.1529/biophysj.104.057364 10.1038/nmeth986 10.1016/S0006-3495(91)82125-7 10.1021/jp052345f 10.1002/bip.1974.360130102 10.1021/ac980027p 10.1103/RevModPhys.15.1 10.1007/BF00185777 |
ContentType | Journal Article |
Copyright | Copyright 2008 The National Academy of Sciences of the United States of America Copyright National Academy of Sciences Nov 25, 2008 2008 by The National Academy of Sciences of the USA |
Copyright_xml | – notice: Copyright 2008 The National Academy of Sciences of the United States of America – notice: Copyright National Academy of Sciences Nov 25, 2008 – notice: 2008 by The National Academy of Sciences of the USA |
DBID | FBQ CGR CUY CVF ECM EIF NPM AAYXX CITATION 7QG 7QL 7QP 7QR 7SN 7SS 7T5 7TK 7TM 7TO 7U9 8FD C1K FR3 H94 M7N P64 RC3 7X8 5PM ADTPV AOWAS D8T ZZAVC |
DOI | 10.1073/pnas.0809250105 |
DatabaseName | AGRIS Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed CrossRef Animal Behavior Abstracts Bacteriology Abstracts (Microbiology B) Calcium & Calcified Tissue Abstracts Chemoreception Abstracts Ecology Abstracts Entomology Abstracts (Full archive) Immunology Abstracts Neurosciences Abstracts Nucleic Acids Abstracts Oncogenes and Growth Factors Abstracts Virology and AIDS Abstracts Technology Research Database Environmental Sciences and Pollution Management Engineering Research Database AIDS and Cancer Research Abstracts Algology Mycology and Protozoology Abstracts (Microbiology C) Biotechnology and BioEngineering Abstracts Genetics Abstracts MEDLINE - Academic PubMed Central (Full Participant titles) SwePub SwePub Articles SWEPUB Freely available online SwePub Articles full text |
DatabaseTitle | MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) CrossRef Virology and AIDS Abstracts Oncogenes and Growth Factors Abstracts Technology Research Database Nucleic Acids Abstracts Ecology Abstracts Neurosciences Abstracts Biotechnology and BioEngineering Abstracts Environmental Sciences and Pollution Management Entomology Abstracts Genetics Abstracts Animal Behavior Abstracts Bacteriology Abstracts (Microbiology B) Algology Mycology and Protozoology Abstracts (Microbiology C) AIDS and Cancer Research Abstracts Chemoreception Abstracts Immunology Abstracts Engineering Research Database Calcium & Calcified Tissue Abstracts MEDLINE - Academic |
DatabaseTitleList | MEDLINE - Academic CrossRef Virology and AIDS Abstracts MEDLINE |
Database_xml | – sequence: 1 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: EIF name: MEDLINE url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search sourceTypes: Index Database – sequence: 3 dbid: FBQ name: AGRIS url: http://www.fao.org/agris/Centre.asp?Menu_1ID=DB&Menu_2ID=DB1&Language=EN&Content=http://www.fao.org/agris/search?Language=EN sourceTypes: Publisher |
DeliveryMethod | fulltext_linktorsrc |
Discipline | Sciences (General) |
EISSN | 1091-6490 |
EndPage | 18181 |
ExternalDocumentID | oai_prod_swepub_kib_ki_se_118018740 1603217951 10_1073_pnas_0809250105 19011092 105_47_18176 25465434 US201301564833 |
Genre | Research Support, Non-U.S. Gov't Journal Article Feature |
GroupedDBID | --- -DZ -~X .55 .GJ 0R~ 123 29P 2AX 2FS 2WC 3O- 4.4 53G 5RE 5VS 692 6TJ 79B 85S AACGO AAFWJ AANCE AAYJJ ABBHK ABOCM ABPLY ABPPZ ABPTK ABTLG ABZEH ACGOD ACIWK ACKIV ACNCT ACPRK ADULT ADZLD AENEX AEUPB AEXZC AFDAS AFFNX AFOSN AFRAH ALMA_UNASSIGNED_HOLDINGS ASUFR AS~ BKOMP CS3 D0L DCCCD DIK DNJUQ DOOOF DU5 DWIUU E3Z EBS EJD F20 F5P FBQ FRP GX1 HGD HH5 HQ3 HTVGU HYE JAAYA JBMMH JENOY JHFFW JKQEH JLS JLXEF JPM JSG JSODD JST KQ8 L7B LU7 MVM N9A NEJ NHB N~3 O9- OK1 P-O PNE PQQKQ R.V RHF RHI RNA RNS RPM RXW SA0 SJN TAE TN5 UKR VOH VQA W8F WH7 WHG WOQ WOW X7M XFK XSW Y6R YBH YKV YSK ZA5 ZCA ZCG ~02 ~KM ABXSQ AQVQM - 02 0R 1AW 55 AAPBV ABFLS ADACO AJYGW AS DZ KM PQEST X XHC ADACV CGR CUY CVF ECM EIF H13 IPSME NPM AAYXX CITATION 7QG 7QL 7QP 7QR 7SN 7SS 7T5 7TK 7TM 7TO 7U9 8FD C1K FR3 H94 M7N P64 RC3 7X8 ADQXQ 5PM ADTPV AOWAS D8T ZZAVC |
ID | FETCH-LOGICAL-c573t-2c82748a3fbe163867d5da4ae4a9b92b2cdf49738bda51e7392d1247f02511713 |
IEDL.DBID | RPM |
ISSN | 0027-8424 1091-6490 |
IngestDate | Wed Oct 23 03:55:24 EDT 2024 Tue Sep 17 21:22:43 EDT 2024 Fri Aug 16 02:04:44 EDT 2024 Thu Oct 10 17:12:43 EDT 2024 Fri Aug 23 00:41:06 EDT 2024 Wed Oct 23 10:01:09 EDT 2024 Wed Nov 11 00:29:45 EST 2020 Thu May 30 08:50:35 EDT 2019 Fri Feb 02 07:05:50 EST 2024 Wed Dec 27 18:59:44 EST 2023 |
IsDoiOpenAccess | true |
IsOpenAccess | true |
IsPeerReviewed | true |
IsScholarly | true |
Issue | 47 |
Language | English |
LinkModel | DirectLink |
MergedId | FETCHMERGED-LOGICAL-c573t-2c82748a3fbe163867d5da4ae4a9b92b2cdf49738bda51e7392d1247f02511713 |
Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Author contributions: R.R. designed research; V.V. performed research; M.H., Y.M., B.J., and L.T. contributed new reagents/analytic tools; V.V. and R.R. analyzed data; and V.V. and R.R. wrote the paper. Parts of this work were presented at the 9th Carl Zeiss-sponsored workshop on FCS and related methods, December 4–6, 2006, AlbaNova University Center, Stockholm Sweden. Communicated by Walter J. Gehring, University of Basel, Basel, Switzerland, October 7, 2008 |
OpenAccessLink | http://kipublications.ki.se/Default.aspx?queryparsed=id:118018740 |
PMID | 19011092 |
PQID | 201389782 |
PQPubID | 42026 |
PageCount | 6 |
ParticipantIDs | pubmed_primary_19011092 swepub_primary_oai_prod_swepub_kib_ki_se_118018740 pnas_primary_105_47_18176_fulltext proquest_journals_201389782 crossref_primary_10_1073_pnas_0809250105 fao_agris_US201301564833 pnas_primary_105_47_18176 proquest_miscellaneous_69832000 jstor_primary_25465434 pubmedcentral_primary_oai_pubmedcentral_nih_gov_2587633 |
ProviderPackageCode | RNA PNE |
PublicationCentury | 2000 |
PublicationDate | 2008-11-25 |
PublicationDateYYYYMMDD | 2008-11-25 |
PublicationDate_xml | – month: 11 year: 2008 text: 2008-11-25 day: 25 |
PublicationDecade | 2000 |
PublicationPlace | United States |
PublicationPlace_xml | – name: United States – name: Washington |
PublicationTitle | Proceedings of the National Academy of Sciences - PNAS |
PublicationTitleAlternate | Proc Natl Acad Sci U S A |
PublicationYear | 2008 |
Publisher | National Academy of Sciences National Acad Sciences |
Publisher_xml | – name: National Academy of Sciences – name: National Acad Sciences |
References | e_1_3_3_17_2 e_1_3_3_16_2 e_1_3_3_19_2 e_1_3_3_18_2 e_1_3_3_13_2 e_1_3_3_12_2 e_1_3_3_15_2 e_1_3_3_14_2 e_1_3_3_11_2 e_1_3_3_10_2 e_1_3_3_6_2 e_1_3_3_5_2 e_1_3_3_8_2 e_1_3_3_7_2 e_1_3_3_27_2 e_1_3_3_24_2 e_1_3_3_23_2 e_1_3_3_26_2 e_1_3_3_25_2 e_1_3_3_2_2 e_1_3_3_20_2 e_1_3_3_1_2 e_1_3_3_4_2 Rigler R (e_1_3_3_9_2) 1993 e_1_3_3_22_2 e_1_3_3_3_2 e_1_3_3_21_2 |
References_xml | – ident: e_1_3_3_12_2 doi: 10.1021/j100036a009 – ident: e_1_3_3_21_2 doi: 10.1126/science.1127344 – ident: e_1_3_3_4_2 doi: 10.1103/PhysRevA.10.1938 – ident: e_1_3_3_15_2 doi: 10.1016/S0006-3495(00)76523-4 – ident: e_1_3_3_14_2 doi: 10.1117/1.1779234 – start-page: 239 volume-title: Laser Spectroscopy of Biomolecules year: 1993 ident: e_1_3_3_9_2 doi: 10.1117/12.146154 contributor: fullname: Rigler R – ident: e_1_3_3_20_2 doi: 10.1038/nmeth922 – ident: e_1_3_3_24_2 doi: 10.1529/biophysj.105.061788 – ident: e_1_3_3_5_2 doi: 10.1073/pnas.91.13.5740 – ident: e_1_3_3_7_2 doi: 10.1016/S0006-3495(94)80801-X – ident: e_1_3_3_25_2 doi: 10.1529/biophysj.107.114645 – ident: e_1_3_3_22_2 doi: 10.1038/nmeth929 – ident: e_1_3_3_3_2 doi: 10.1016/0301-0104(74)85005-6 – ident: e_1_3_3_26_2 doi: 10.1016/S0006-3495(99)76912-2 – ident: e_1_3_3_10_2 doi: 10.2174/138920106777950843 – ident: e_1_3_3_17_2 doi: 10.1073/pnas.96.24.13756 – ident: e_1_3_3_27_2 doi: 10.1073/pnas.0606557104 – ident: e_1_3_3_23_2 doi: 10.1007/s12013-007-9000-5 – ident: e_1_3_3_1_2 doi: 10.1103/PhysRevLett.29.705 – ident: e_1_3_3_11_2 doi: 10.1529/biophysj.104.057364 – ident: e_1_3_3_8_2 doi: 10.1038/nmeth986 – ident: e_1_3_3_19_2 doi: 10.1016/S0006-3495(91)82125-7 – ident: e_1_3_3_16_2 doi: 10.1021/jp052345f – ident: e_1_3_3_2_2 doi: 10.1002/bip.1974.360130102 – ident: e_1_3_3_13_2 doi: 10.1021/ac980027p – ident: e_1_3_3_18_2 doi: 10.1103/RevModPhys.15.1 – ident: e_1_3_3_6_2 doi: 10.1007/BF00185777 |
SSID | ssj0009580 |
Score | 2.2722497 |
Snippet | A new approach to quantitative single-molecule imaging by confocal laser scanning microscopy (CLSM) is presented. It relies on fluorescence intensity... |
SourceID | swepub pubmedcentral proquest crossref pubmed pnas jstor fao |
SourceType | Open Access Repository Aggregation Database Index Database Enrichment Source Publisher |
StartPage | 18176 |
SubjectTerms | Cells Correlations Deoxyribonucleic acid DNA DNA - chemistry Fluorescence Fluorescent Dyes Image analysis Imaging Lasers Medicin och hälsovetenskap Microscopy Microscopy, Confocal - methods Molecular imaging Molecular structure Molecules Photons Physical Sciences Pixels Proteins Spectroscopy |
Title | Quantitative single-molecule imaging by confocal laser scanning microscopy |
URI | https://www.jstor.org/stable/25465434 http://www.pnas.org/content/105/47/18176.abstract https://www.ncbi.nlm.nih.gov/pubmed/19011092 https://www.proquest.com/docview/201389782 https://search.proquest.com/docview/69832000 https://pubmed.ncbi.nlm.nih.gov/PMC2587633 http://kipublications.ki.se/Default.aspx?queryparsed=id:118018740 |
Volume | 105 |
hasFullText | 1 |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Lb9QwEB51e-KCaKHUlBYLcSiH7MOPODmiiqoUFYFgpd4sO3boiia7anYP_feMnWRXEXDhkEscO4pn7PkmnvkG4B1zM2tNoRLDrUhEyaeJZcwlKp0WuZBG2ViS5eZLejUX17fydg9knwsTg_YLuxjX99W4XtzF2MpVVUz6OLHJ15sLJgOPGp-MYKQ47130LdNu1uadMNx-BRM9n4_ik1VtmjFCpBztPuKKSBga7F_OBlZpVJplH54YOE-x19_w559hlAOy0WigLp_B0w5Z0g_tFxzAnq8P4aBbuw097wim3z-H628bU8fkMtzqaPhZcO-Tqq2T6-miioWLqH2k6CuXwdZRhNj-gTZFW-CIViGKL-SzPL6A-eXHHxdXSVdTISmk4uuEFRn6oZnhpfUBiqXKSWeE8cLkNmeWFa4UueKZdUbOvEL45BACqDL6IujRHsF-vaz9MdBScFOayHHmBXpNuXSILnMzVYo7FDSB835O9aqlztDxyFtxHeZU7yRB4BjnXJufuLHp-XcWjlMDi03GOYGjKIjtEIHAP-TDEiBxlN3QUgulEbGolMDbf7bpsouqIXDSy1R3C7fRLJ7cImwi8GbbiisuHKOY2i83jU5z3AVR0Qi8bOW_e02nTQTUQDO2DwQu72ELqnjk9O5UmgBrdWjYBW2p7u7_WoRLN14H8r5YUfHVf7_uBJ7E-JfZLGHyNeyvHzb-FEHW2p6he_Hp81lcWr8BNdgjsg |
link.rule.ids | 230,315,733,786,790,891,27955,27956,53825,53827 |
linkProvider | National Library of Medicine |
linkToHtml | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Lb9QwEB615QAXoEBbU6AR4lAOye76ESdHVFEtpVuB6KLeLDtxYNUmu2p2D-XXM3aSXYXHAQ65xIkja8Yz38Qz3wC8ofnIGJ3JUDPDQ16wYWgozUMZD7OUCy2Nb8kyuYjHU352Ja62QHS1MD5pPzOzqLopo2r23edWLsps0OWJDT5NTqhwPGpssA33cL9S0QXpa67dpKk8oWiAOeUdo49kg0Wl6whBUoqeH5GFpwx1HjClPb-0Xeh5l6DoWE_xrT8h0N8TKXt0o95FnT6Cr93imsyU62i1NFH24xfex39e_WN42ILW4F0zvAtbtnoCu61ZqIPjlrv67VM4-7zSla9bQysauP8QNzYsmxa8NpiVvidSYO4CDMML50YDRO_2NqizpndSULoEQVcqc_cMpqfvL0_GYduuIcyEZMuQZgmGuIlmhbEO5cUyF7nm2nKdmpQamuUFTyVLTK7FyEpEZjmiC1n4MAeD5T3YqeaVPYCg4EwX2tOnWY4BWSpyBK6pHkrJctQhAsedsNSiYeVQ_jRdMuWEpTYiJnCAwlT6G9pMNf1C3UmtI8hJGCOw5yW8nsL1BnCltgSIn2UztVBcKgRDMibw-q9jqmgTdggcdsqiWptQK-oPhRGREThaj-Jmdic0urLzVa3iFA0sajCB_UaxNp9p1ZSA7Knc-gFHE94fQf3xdOGtvhCgjXL2X0E3rdr71zN3qdoqxwvomzU-_-_PHcH98eXkXJ1_uPh4CA98ms1oFFLxAnaWtyv7ErHc0rzyO_cnmmpEsQ |
linkToPdf | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Lb9QwEB7RIiEulAJtTYFGiEM5ZB-2E2-OqLAqhVZFsFLFxbJjG1Ztsqtm99D-esZOskt4XHrIJX5EyYxnvonH3wC8oWaotcpFrJjmMXdsEGtKTSzSQZ7xRAkdSrKcnqXHE35ykVz8VuorJO3netorr4peOf0ZcivnRd5v88T656dHNPE8aqw_N66_AfdxzVLRBuorvt1RffqEohHmlLesPgJHlarqIVDK0Psjugi0od4LZrTjmzacmrVJip75FEf9C4X-nUzZoRwNbmq8Bd_bF6yzUy57y4Xu5bd_cD_e6Qs8hkcNeI3e1V224Z4tn8B2Yx6q6LDhsH77FE6-LFUZzq-hNY38_4grGxd1KV4bTYtQGynSNxGG48670whRvL2OqryuoRQVPlHQH5m5eQaT8YdvR8dxU7YhzhPBFjHNURx8pJjT1qO9VJjEKK4sV5nOqKa5cTwTbKSNSoZWIEIziDKEC-EOBs07sFnOSrsHkeNMORVo1CzHwCxLDALYTA2EYAZ1icBhKzA5r9k5ZNhVF0x6gcm1mAnsoUCl-oG2U06-Ur9j64lyRowR2AlSXk3hawT4I7cESJhlPXUiuZAIikRK4PV_26RrEncI7LcKIxvbUEkaNocRmRE4WLXiovY7Naq0s2Ul0wwNLWoxgd1audaPaVSVgOio3aqDpwvvtqAOBdrwRmcI0FpBu0PQXcvm_uXUX7Ky0vMDhqKNz-_8uAN4cP5-LD9_PPu0Dw9Dts1wGNPkBWwurpf2JUK6hX4VFu8vyxNHMQ |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Quantitative+single-molecule+imaging+by+confocal+laser+scanning+microscopy&rft.jtitle=Proceedings+of+the+National+Academy+of+Sciences+-+PNAS&rft.au=Vladana+Vukojevi%C4%87&rft.au=Marcus+Heidkamp&rft.au=Yu+Ming&rft.au=Bj%C3%B6rn+Johansson&rft.date=2008-11-25&rft.pub=National+Acad+Sciences&rft.issn=0027-8424&rft.eissn=1091-6490&rft.volume=105&rft.issue=47&rft.spage=18176&rft_id=info:doi/10.1073%2Fpnas.0809250105&rft_id=info%3Apmid%2F19011092&rft.externalDBID=n%2Fa&rft.externalDocID=105_47_18176 |
thumbnail_m | http://utb.summon.serialssolutions.com/2.0.0/image/custom?url=http%3A%2F%2Fwww.pnas.org%2Fcontent%2F105%2F47.cover.gif |
thumbnail_s | http://utb.summon.serialssolutions.com/2.0.0/image/custom?url=http%3A%2F%2Fwww.pnas.org%2Fcontent%2F105%2F47.cover.gif |