MPLEx: a method for simultaneous pathogen inactivation and extraction of samples for multi-omics profiling

The continued emergence and spread of infectious agents is of great concern, and systems biology approaches to infectious disease research can advance our understanding of host-pathogen relationships and facilitate the development of new therapies and vaccines. Molecular characterization of infectio...

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Published inAnalyst Vol. 142; no. 3; pp. 442 - 448
Main Authors Burnum-Johnson, Kristin E, Kyle, Jennifer E, Eisfeld, Amie J, Casey, Cameron P, Stratton, Kelly G, Gonzalez, Juan F, Habyarimana, Fabien, Negretti, Nicholas M, Sims, Amy C, Chauhan, Sadhana, Thackray, Larissa B, Halfmann, Peter J, Walters, Kevin B, Kim, Young-Mo, Zink, Erika M, Nicora, Carrie D, Weitz, Karl K, Webb-Robertson, Bobbie-Jo M, Nakayasu, Ernesto S, Ahmer, Brian, Konkel, Michael E, Motin, Vladimir, Baric, Ralph S, Diamond, Michael S, Kawaoka, Yoshihiro, Waters, Katrina M, Smith, Richard D, Metz, Thomas O
Format Journal Article Web Resource
LanguageEnglish
Published England Royal Society of Chemistry 26.01.2017
Subjects
Bacteria
Bacteria - isolation & purification
BASIC BIOLOGICAL SCIENCES
Cell Line
Environmental Molecular Sciences Laboratory
Epithelial Cells
Exposure
Extraction
Humans
Inactivation
Lipids
Lipids - analysis
Mass Spectrometry
Membranes
Metabolomics
Pathogens
Proteins
Proteomics
Virus Inactivation
Viruses
Viruses - isolation & purification
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Abstract The continued emergence and spread of infectious agents is of great concern, and systems biology approaches to infectious disease research can advance our understanding of host-pathogen relationships and facilitate the development of new therapies and vaccines. Molecular characterization of infectious samples outside of appropriate biosafety containment can take place only subsequent to pathogen inactivation. Herein, we describe a modified Folch extraction using chloroform/methanol that facilitates the molecular characterization of infectious samples by enabling simultaneous pathogen inactivation and extraction of proteins, metabolites, and lipids for subsequent mass spectrometry-based multi-omics measurements. This single-sample metabolite, protein and lipid extraction (MPLEx) method resulted in complete inactivation of clinically important bacterial and viral pathogens with exposed lipid membranes, including Yersinia pestis, Salmonella Typhimurium, and Campylobacter jejuni in pure culture, and Yersinia pestis, Campylobacter jejuni, and West Nile, MERS-CoV, Ebola, and influenza H7N9 viruses in infection studies. In addition, >99% inactivation, which increased with solvent exposure time, was also observed for pathogens without exposed lipid membranes including community-associated methicillin-resistant Staphylococcus aureus, Clostridium difficile spores and vegetative cells, and adenovirus type 5. The overall pipeline of inactivation and subsequent proteomic, metabolomic, and lipidomic analyses was evaluated using a human epithelial lung cell line infected with wild-type and mutant influenza H7N9 viruses, thereby demonstrating that MPLEx yields biomaterial of sufficient quality for subsequent multi-omics analyses. Based on these experimental results, we believe that MPLEx will facilitate systems biology studies of infectious samples by enabling simultaneous pathogen inactivation and multi-omics measurements from a single specimen with high success for pathogens with exposed lipid membranes.
AbstractList The continued emergence and spread of infectious agents is of great concern, and systems biology approaches to infectious disease research can advance our understanding of host–pathogen relationships and facilitate the development of new therapies and vaccines. Molecular characterization of infectious samples outside of appropriate biosafety containment can take place only subsequent to pathogen inactivation. Herein, we describe a modified Folch extraction using chloroform/methanol that facilitates the molecular characterization of infectious samples by enabling simultaneous pathogen inactivation and extraction of proteins, metabolites, and lipids for subsequent mass spectrometry-based multi-omics measurements. This single-sample metabolite, protein and lipid extraction (MPLEx) method resulted in complete inactivation of clinically important bacterial and viral pathogens with exposed lipid membranes, including Yersinia pestis , Salmonella Typhimurium, and Campylobacter jejuni in pure culture, and Yersinia pestis , Campylobacter jejuni , and West Nile, MERS-CoV, Ebola, and influenza H7N9 viruses in infection studies. In addition, >99% inactivation, which increased with solvent exposure time, was also observed for pathogens without exposed lipid membranes including community-associated methicillin-resistant Staphylococcus aureus , Clostridium difficile spores and vegetative cells, and adenovirus type 5. The overall pipeline of inactivation and subsequent proteomic, metabolomic, and lipidomic analyses was evaluated using a human epithelial lung cell line infected with wild-type and mutant influenza H7N9 viruses, thereby demonstrating that MPLEx yields biomaterial of sufficient quality for subsequent multi-omics analyses. Based on these experimental results, we believe that MPLEx will facilitate systems biology studies of infectious samples by enabling simultaneous pathogen inactivation and multi-omics measurements from a single specimen with high success for pathogens with exposed lipid membranes.
The continued emergence and spread of infectious agents is of great concern, and systems biology approaches to infectious disease research can advance our understanding of host-pathogen relationships and facilitate the development of new therapies and vaccines. Molecular characterization of infectious samples outside of appropriate biosafety containment can take place only subsequent to pathogen inactivation. Herein, we describe a modified Folch extraction using chloroform/methanol that facilitates the molecular characterization of infectious samples by enabling simultaneous pathogen inactivation and extraction of proteins, metabolites, and lipids for subsequent mass spectrometry-based multi-omics measurements. This single-sample metabolite, protein and lipid extraction (MPLEx) method resulted in complete inactivation of clinically important bacterial and viral pathogens with exposed lipid membranes, including Yersinia pestis, Salmonella Typhimurium, and Campylobacter jejuni in pure culture, and Yersinia pestis, Campylobacter jejuni, and West Nile, MERS-CoV, Ebola, and influenza H7N9 viruses in infection studies. In addition, >99% inactivation, which increased with solvent exposure time, was also observed for pathogens without exposed lipid membranes including community-associated methicillin-resistant Staphylococcus aureus, Clostridium difficile spores and vegetative cells, and adenovirus type 5. The overall pipeline of inactivation and subsequent proteomic, metabolomic, and lipidomic analyses was evaluated using a human epithelial lung cell line infected with wild-type and mutant influenza H7N9 viruses, thereby demonstrating that MPLEx yields biomaterial of sufficient quality for subsequent multi-omics analyses. Based on these experimental results, we believe that MPLEx will facilitate systems biology studies of infectious samples by enabling simultaneous pathogen inactivation and multi-omics measurements from a single specimen with high success for pathogens with exposed lipid membranes.
The continued emergence and spread of infectious agents is of increasing concern due to increased population growth and the associated increased livestock production to meet food demands, increased urbanization and land-use changes, and greater travel. A systems biology approach to infectious disease research can significantly advance our understanding of host-pathogen relationships and facilitate the development of new therapies and vaccines. Molecular characterization of infectious samples outside of appropriate biosafety containment can only take place subsequent to pathogen inactivation. Herein, we describe a modified Folch extraction using chloroform/methanol that facilitates the molecular characterization of infectious samples by enabling simultaneous pathogen inactivation and extraction of proteins, metabolites, and lipids for subsequent mass spectrometry-based multi-omics measurements. This metabolite, protein and lipid extraction (MPLEx) method resulted in complete inactivation of bacterial and viral pathogens with exposed lipid membranes, including Yersinia pestis, Salmonella Typhimurium, and Campylobacter jejuni in pure culture, and Yersinia pestis, Campylobacter jejuni, West Nile, MERS-CoV, Ebola, and influenza H7N9 viruses in infection studies. Partial inactivation was observed for pathogens without exposed lipid membranes including 99.99% inactivation of community-associated methicillin-resistant Staphylococcus aureus, 99.6% and >99% inactivation of Clostridium difficile spores and vegetative cells, respectively, and 50% inactivation of adenovirus type 5. To demonstrate that MPLEx yields biomaterial of sufficient quality for subsequent multi-omics analyses, we highlight select proteomics, metabolomics and lipidomics data from human epithelial lung cells infected with wild-type and mutant forms of influenza H7N9. We believe that MPLEx will facilitate systems biology studies of infectious samples by enabling simultaneous pathogen inactivation and multi-omics measurements from a single specimen.
Author Weitz, Karl K
Waters, Katrina M
Casey, Cameron P
Sims, Amy C
Motin, Vladimir
Konkel, Michael E
Nicora, Carrie D
Thackray, Larissa B
Ahmer, Brian
Smith, Richard D
Chauhan, Sadhana
Kim, Young-Mo
Webb-Robertson, Bobbie-Jo M
Metz, Thomas O
Stratton, Kelly G
Negretti, Nicholas M
Zink, Erika M
Eisfeld, Amie J
Habyarimana, Fabien
Walters, Kevin B
Kyle, Jennifer E
Baric, Ralph S
Gonzalez, Juan F
Nakayasu, Ernesto S
Kawaoka, Yoshihiro
Burnum-Johnson, Kristin E
Halfmann, Peter J
Diamond, Michael S
AuthorAffiliation 2 Infuenza Research Institute, Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, WI
8 Computational and Statistical Analytics Division, Pacific Northwest National Laboratory, Richland, WA
4 School of Molecular Biosciences, College of Veterinary Medicine, Washington State University, Pullman, WA
7 Departments of Medicine, Molecular Microbiology, Pathology & Immunology, Washington University School of Medicine, St. Louis, MO
1 Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA
3 Department of Microbial Infection and Immunity, Ohio State University, Columbus, OH
5 Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC
6 Department of Pathology, University of Texas Medical Branch, Galveston, TX
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BackLink https://www.ncbi.nlm.nih.gov/pubmed/28091625$$D View this record in MEDLINE/PubMed
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Cites_doi 10.1128/JVI.00570-14
10.1053/jinf.2000.0652
10.1038/nrmicro2921
10.1128/mBio.00325-10
10.1016/j.cub.2010.06.031
10.1128/JVI.14.3.689-699.1974
10.1016/S0140-6736(06)68853-3
10.1128/JVI.01087-15
10.1073/pnas.2235620100
10.1016/j.tim.2014.04.003
10.1038/28970
10.1016/j.jbbm.2003.10.008
10.1146/annurev-immunol-032713-120254
10.1146/annurev.micro.61.080706.093224
10.1046/j.1537-2995.1985.25686071422.x
10.1128/AAC.01498-09
10.1016/S0021-9258(18)64849-5
10.1073/pnas.0306859101
10.1056/NEJMsa0806477
10.1128/IAI.61.4.1251-1258.1993
10.1016/j.febslet.2006.07.007
10.1128/JVI.11.5.799-805.1973
10.1128/AEM.17.3.379-383.1969
10.1002/jps.20157
10.3181/00379727-106-26459
10.1021/ac8016899
10.1128/mSystems.00043-16
10.1128/IAI.41.2.816-821.1983
10.1038/nature06536
10.1016/S1359-6446(05)03735-9
10.1086/509936
10.1086/521911
10.1016/j.beha.2005.04.001
10.1111/j.1750-3841.2009.01393.x
10.1073/pnas.1407087111
10.1002/nbm.740
10.1016/j.rinim.2012.01.002
10.1016/j.trsl.2011.12.009
10.1177/003335491212700305
10.1016/j.virusres.2013.02.011
10.1083/jcb.200205044
10.1021/pr1005247
10.1159/000323552
10.1016/j.jviromet.2006.05.027
10.1126/science.287.5452.443
10.1016/j.chroma.2003.10.029
10.1038/nature09575
10.1371/journal.ppat.1003467
10.1371/journal.pntd.0000300
10.1056/NEJMp1209051
10.1128/JVI.27.2.432-435.1978
10.1126/science.aaa4339
10.1128/AEM.00487-08
10.1086/423182
10.1126/science.1177345
10.1128/JB.00369-13
10.1021/ac9019522
10.1016/j.jhin.2010.06.028
10.1139/y59-099
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References 22894554 - N Engl J Med. 2012 Aug 30;367(9):785-7
24814671 - Trends Microbiol. 2014 Jul;22(7):406-16
27822525 - mSystems. 2016 May 10;1(3):null
8454328 - Infect Immun. 1993 Apr;61(4):1251-8
6409813 - Infect Immun. 1983 Aug;41(2):816-21
17879923 - Clin Infect Dis. 2007 Oct 15;45(8):1039-46
21285433 - MBio. 2011 Mar 01;2(1):e00325-10
18035610 - Annu Rev Microbiol. 2007;61:555-88
20850897 - J Hosp Infect. 2011 Mar;77(3):204-9
4211861 - J Virol. 1974 Sep;14(3):689-99
25288733 - Proc Natl Acad Sci U S A. 2014 Oct 21;111(42):15214-9
20833318 - Curr Biol. 2010 Sep 14;20(17):R735-45
3934801 - Transfusion. 1985 Nov-Dec;25(6):516-22
20831241 - J Proteome Res. 2010 Nov 5;9(11):5748-56
20047916 - Antimicrob Agents Chemother. 2010 Mar;54(3):1363-4
16789137 - J Virol. 1973 May;11(5):799-805
11840551 - NMR Biomed. 2002 Feb;15(1):37-44
18586970 - Appl Environ Microbiol. 2008 Aug;74(16):5047-52
10908015 - J Infect. 2000 May;40(3):218-28
21779207 - Transfus Med Hemother. 2011;38(1):65-70
12163463 - J Cell Biol. 2002 Aug 5;158(3):395-9
19928838 - Anal Chem. 2009 Dec 15;81(24):10038-48
13428781 - J Biol Chem. 1957 May;226(1):497-509
17278063 - Clin Infect Dis. 2007 Mar 1;44(5):701-3
23853589 - PLoS Pathog. 2013;9(7):e1003467
23499671 - Virus Res. 2013 Dec 5;178(1):151-67
19061395 - Anal Chem. 2009 Jan 1;81(1):174-83
16377551 - Best Pract Res Clin Haematol. 2006;19(1):205-42
15126669 - Proc Natl Acad Sci U S A. 2004 May 18;101(20):7733-8
15356786 - Clin Infect Dis. 2004 Sep 1;39(5):702-9
15026205 - J Biochem Biophys Methods. 2004 Mar 31;58(3):187-93
21124449 - Nature. 2010 Dec 2;468(7324):647-52
19420367 - N Engl J Med. 2009 May 7;360(19):1981-8
16808980 - J Virol Methods. 2006 Oct;137(1):160-3
15349956 - J Pharm Sci. 2004 Oct;93(10):2458-75
22633095 - Transl Res. 2012 Jun;159(6):430-53
10642539 - Science. 2000 Jan 21;287(5452):443-9
23794627 - J Bacteriol. 2013 Sep;195(17):3863-75
16580599 - Drug Discov Today. 2006 Mar;11(5-6):220-7
16859687 - FEBS Lett. 2006 Oct 9;580(23):5541-51
20492187 - J Food Sci. 2010 Jan-Feb;75(1):M7-13
13671378 - Can J Biochem Physiol. 1959 Aug;37(8):911-7
26136573 - J Virol. 2015 Sep;89(18):9440-53
24991006 - J Virol. 2014 Sep;88(18):10556-68
14753699 - J Chromatogr A. 2004 Jan 16;1023(2):317-20
5780397 - Appl Microbiol. 1969 Mar;17(3):379-83
14561897 - Proc Natl Acad Sci U S A. 2003 Dec 9;100(25):14610-7
19965751 - Science. 2009 Dec 4;326(5958):1362-7
16950365 - Lancet. 2006 Sep 2;368(9538):874-85
24371563 - Results Immunol. 2012 Jan 16;2:19-24
18820747 - PLoS Negl Trop Dis. 2008 Sep 24;2(9):e300
9707111 - Nature. 1998 Aug 6;394(6693):531-2
691117 - J Virol. 1978 Aug;27(2):432-5
22547856 - Public Health Rep. 2012 May-Jun;127(3):259-66
24655298 - Annu Rev Immunol. 2014;32:547-77
13698695 - Proc Soc Exp Biol Med. 1961 Apr;106:736-8
23202530 - Nat Rev Microbiol. 2013 Jan;11(1):33-44
18288193 - Nature. 2008 Feb 21;451(7181):990-3
Maillard (C6AN02486F-(cit22)/*[position()=1]) 2011; 77
Adu-Gyamfi (C6AN02486F-(cit60)/*[position()=1]) 2015; 89
Heesterbeek (C6AN02486F-(cit1)/*[position()=1]) 2015; 347
Paredes-Sabja (C6AN02486F-(cit58)/*[position()=1]) 2014; 22
Macinga (C6AN02486F-(cit38)/*[position()=1]) 2008; 74
Feinstone (C6AN02486F-(cit28)/*[position()=1]) 1983; 41
Forst (C6AN02486F-(cit16)/*[position()=1]) 2006; 11
Webb-Robertson (C6AN02486F-(cit49)/*[position()=1]) 2010; 9
de Jong (C6AN02486F-(cit7)/*[position()=1]) 2000; 40
McKenney (C6AN02486F-(cit56)/*[position()=1]) 2013; 11
de Hoon (C6AN02486F-(cit57)/*[position()=1]) 2010; 20
Jones (C6AN02486F-(cit3)/*[position()=1]) 2008; 451
Bligh (C6AN02486F-(cit41)/*[position()=1]) 1959; 37
Hotez (C6AN02486F-(cit14)/*[position()=1]) 2008; 2
Josset (C6AN02486F-(cit18)/*[position()=1]) 2013; 178
Parisius (C6AN02486F-(cit30)/*[position()=1]) 1969; 17
Cham (C6AN02486F-(cit32)/*[position()=1]) 2006; 137
Ruiz-Palacios (C6AN02486F-(cit8)/*[position()=1]) 2007; 44
Omer (C6AN02486F-(cit11)/*[position()=1]) 2009; 360
Pelletier (C6AN02486F-(cit34)/*[position()=1]) 2006; 19
Ferraz (C6AN02486F-(cit44)/*[position()=1]) 2004; 58
Bergey (C6AN02486F-(cit52)/*[position()=1]) 1984
Wenk (C6AN02486F-(cit20)/*[position()=1]) 2006; 580
FELDMAN (C6AN02486F-(cit25)/*[position()=1]) 1961; 106
Horowitz (C6AN02486F-(cit33)/*[position()=1]) 1985; 25
Jiang (C6AN02486F-(cit45)/*[position()=1]) 2004; 1023
Cowan (C6AN02486F-(cit53)/*[position()=1]) 2004; 101
Keesing (C6AN02486F-(cit5)/*[position()=1]) 2010; 468
Millet (C6AN02486F-(cit59)/*[position()=1]) 2014; 111
Aderem (C6AN02486F-(cit15)/*[position()=1]) 2011; 2
Favero (C6AN02486F-(cit23)/*[position()=1]) 2001
Lloyd-Smith (C6AN02486F-(cit4)/*[position()=1]) 2009; 326
Ackermann (C6AN02486F-(cit62)/*[position()=1]) 2006
Cherry (C6AN02486F-(cit10)/*[position()=1]) 2012; 367
Dichtelmüller (C6AN02486F-(cit35)/*[position()=1]) 2012; 2
Du (C6AN02486F-(cit37)/*[position()=1]) 2010; 75
Turner (C6AN02486F-(cit39)/*[position()=1]) 2010; 54
Grundmann (C6AN02486F-(cit9)/*[position()=1]) 2006; 368
Bouri (C6AN02486F-(cit13)/*[position()=1]) 2012; 127
Evans (C6AN02486F-(cit31)/*[position()=1]) 2004; 93
McMurry (C6AN02486F-(cit24)/*[position()=1]) 1998; 394
Olsen (C6AN02486F-(cit27)/*[position()=1]) 1974; 14
Folch (C6AN02486F-(cit40)/*[position()=1]) 1957; 226
Belle (C6AN02486F-(cit43)/*[position()=1]) 2002; 15
Morrison (C6AN02486F-(cit51)/*[position()=1]) 2014; 88
Takeda (C6AN02486F-(cit61)/*[position()=1]) 2003; 100
Nakayasu (C6AN02486F-(cit46)/*[position()=1]) 2016; 1
Fontana (C6AN02486F-(cit19)/*[position()=1]) 2012; 159
Rutala (C6AN02486F-(cit21)/*[position()=1]) 2004; 39
Vidaver (C6AN02486F-(cit26)/*[position()=1]) 1973; 11
Fay (C6AN02486F-(cit42)/*[position()=1]) 1978; 27
Henriques (C6AN02486F-(cit55)/*[position()=1]) 2007; 61
Daszak (C6AN02486F-(cit2)/*[position()=1]) 2000; 287
Zak (C6AN02486F-(cit17)/*[position()=1]) 2014; 32
Sellick (C6AN02486F-(cit47)/*[position()=1]) 2009; 81
Gubler (C6AN02486F-(cit12)/*[position()=1]) 2007; 45
Barra-Carrasco (C6AN02486F-(cit54)/*[position()=1]) 2013; 195
Fries (C6AN02486F-(cit29)/*[position()=1]) 1993; 61
Bushell (C6AN02486F-(cit50)/*[position()=1]) 2002; 158
Hellstern (C6AN02486F-(cit36)/*[position()=1]) 2011; 38
Kind (C6AN02486F-(cit48)/*[position()=1]) 2009; 81
Morens (C6AN02486F-(cit6)/*[position()=1]) 2013; 9
References_xml – volume: 88
  start-page: 10556
  year: 2014
  ident: C6AN02486F-(cit51)/*[position()=1]
  publication-title: J. Virol.
  doi: 10.1128/JVI.00570-14
  contributor:
    fullname: Morrison
– volume: 40
  start-page: 218
  year: 2000
  ident: C6AN02486F-(cit7)/*[position()=1]
  publication-title: J. Infect.
  doi: 10.1053/jinf.2000.0652
  contributor:
    fullname: de Jong
– volume: 11
  start-page: 33
  year: 2013
  ident: C6AN02486F-(cit56)/*[position()=1]
  publication-title: Nat. Rev. Microbiol.
  doi: 10.1038/nrmicro2921
  contributor:
    fullname: McKenney
– volume: 2
  start-page: 1e00325
  year: 2011
  ident: C6AN02486F-(cit15)/*[position()=1]
  publication-title: mBio
  doi: 10.1128/mBio.00325-10
  contributor:
    fullname: Aderem
– volume: 20
  start-page: R735
  year: 2010
  ident: C6AN02486F-(cit57)/*[position()=1]
  publication-title: Curr. Biol.
  doi: 10.1016/j.cub.2010.06.031
  contributor:
    fullname: de Hoon
– volume: 14
  start-page: 689
  year: 1974
  ident: C6AN02486F-(cit27)/*[position()=1]
  publication-title: J. Virol.
  doi: 10.1128/JVI.14.3.689-699.1974
  contributor:
    fullname: Olsen
– volume: 368
  start-page: 874
  year: 2006
  ident: C6AN02486F-(cit9)/*[position()=1]
  publication-title: Lancet
  doi: 10.1016/S0140-6736(06)68853-3
  contributor:
    fullname: Grundmann
– volume: 89
  start-page: 9440
  issue: 18
  year: 2015
  ident: C6AN02486F-(cit60)/*[position()=1]
  publication-title: J. Virol.
  doi: 10.1128/JVI.01087-15
  contributor:
    fullname: Adu-Gyamfi
– volume: 100
  start-page: 14610
  year: 2003
  ident: C6AN02486F-(cit61)/*[position()=1]
  publication-title: Proc. Natl. Acad. Sci. U. S. A.
  doi: 10.1073/pnas.2235620100
  contributor:
    fullname: Takeda
– volume: 22
  start-page: 406
  year: 2014
  ident: C6AN02486F-(cit58)/*[position()=1]
  publication-title: Trends Microbiol.
  doi: 10.1016/j.tim.2014.04.003
  contributor:
    fullname: Paredes-Sabja
– volume: 394
  start-page: 531
  year: 1998
  ident: C6AN02486F-(cit24)/*[position()=1]
  publication-title: Nature
  doi: 10.1038/28970
  contributor:
    fullname: McMurry
– volume: 58
  start-page: 187
  year: 2004
  ident: C6AN02486F-(cit44)/*[position()=1]
  publication-title: J. Biochem. Biophys. Methods
  doi: 10.1016/j.jbbm.2003.10.008
  contributor:
    fullname: Ferraz
– volume: 32
  start-page: 547
  year: 2014
  ident: C6AN02486F-(cit17)/*[position()=1]
  publication-title: Annu. Rev. Immunol.
  doi: 10.1146/annurev-immunol-032713-120254
  contributor:
    fullname: Zak
– volume: 61
  start-page: 555
  year: 2007
  ident: C6AN02486F-(cit55)/*[position()=1]
  publication-title: Annu. Rev. Microbiol.
  doi: 10.1146/annurev.micro.61.080706.093224
  contributor:
    fullname: Henriques
– volume: 25
  start-page: 516
  year: 1985
  ident: C6AN02486F-(cit33)/*[position()=1]
  publication-title: Transfusion
  doi: 10.1046/j.1537-2995.1985.25686071422.x
  contributor:
    fullname: Horowitz
– volume: 54
  start-page: 1363
  year: 2010
  ident: C6AN02486F-(cit39)/*[position()=1]
  publication-title: Antimicrob. Agents Chemother.
  doi: 10.1128/AAC.01498-09
  contributor:
    fullname: Turner
– volume: 226
  start-page: 497
  year: 1957
  ident: C6AN02486F-(cit40)/*[position()=1]
  publication-title: J. Biol. Chem.
  doi: 10.1016/S0021-9258(18)64849-5
  contributor:
    fullname: Folch
– volume: 101
  start-page: 7733
  year: 2004
  ident: C6AN02486F-(cit53)/*[position()=1]
  publication-title: Proc. Natl. Acad. Sci. U. S. A.
  doi: 10.1073/pnas.0306859101
  contributor:
    fullname: Cowan
– volume: 360
  start-page: 1981
  year: 2009
  ident: C6AN02486F-(cit11)/*[position()=1]
  publication-title: N. Engl. J. Med.
  doi: 10.1056/NEJMsa0806477
  contributor:
    fullname: Omer
– volume: 61
  start-page: 1251
  year: 1993
  ident: C6AN02486F-(cit29)/*[position()=1]
  publication-title: Infect. Immun.
  doi: 10.1128/IAI.61.4.1251-1258.1993
  contributor:
    fullname: Fries
– volume: 580
  start-page: 5541
  year: 2006
  ident: C6AN02486F-(cit20)/*[position()=1]
  publication-title: FEBS Lett.
  doi: 10.1016/j.febslet.2006.07.007
  contributor:
    fullname: Wenk
– volume-title: Classification of bacteriophages, in The Bacteriophages
  year: 2006
  ident: C6AN02486F-(cit62)/*[position()=1]
  contributor:
    fullname: Ackermann
– volume: 11
  start-page: 799
  year: 1973
  ident: C6AN02486F-(cit26)/*[position()=1]
  publication-title: J. Virol.
  doi: 10.1128/JVI.11.5.799-805.1973
  contributor:
    fullname: Vidaver
– volume-title: Disinfection, sterilization, and preservation
  year: 2001
  ident: C6AN02486F-(cit23)/*[position()=1]
  contributor:
    fullname: Favero
– volume: 17
  start-page: 379
  year: 1969
  ident: C6AN02486F-(cit30)/*[position()=1]
  publication-title: Appl. Microbiol.
  doi: 10.1128/AEM.17.3.379-383.1969
  contributor:
    fullname: Parisius
– volume: 93
  start-page: 2458
  year: 2004
  ident: C6AN02486F-(cit31)/*[position()=1]
  publication-title: J. Pharm. Sci.
  doi: 10.1002/jps.20157
  contributor:
    fullname: Evans
– volume: 106
  start-page: 736
  year: 1961
  ident: C6AN02486F-(cit25)/*[position()=1]
  publication-title: Proc. Soc. Exp. Biol. Med.
  doi: 10.3181/00379727-106-26459
  contributor:
    fullname: FELDMAN
– volume: 81
  start-page: 174
  year: 2009
  ident: C6AN02486F-(cit47)/*[position()=1]
  publication-title: Anal. Chem.
  doi: 10.1021/ac8016899
  contributor:
    fullname: Sellick
– volume: 1
  start-page: e00043
  issue: 3
  year: 2016
  ident: C6AN02486F-(cit46)/*[position()=1]
  publication-title: mSystems
  doi: 10.1128/mSystems.00043-16
  contributor:
    fullname: Nakayasu
– volume: 41
  start-page: 816
  year: 1983
  ident: C6AN02486F-(cit28)/*[position()=1]
  publication-title: Infect. Immun.
  doi: 10.1128/IAI.41.2.816-821.1983
  contributor:
    fullname: Feinstone
– volume: 451
  start-page: 990
  year: 2008
  ident: C6AN02486F-(cit3)/*[position()=1]
  publication-title: Nature
  doi: 10.1038/nature06536
  contributor:
    fullname: Jones
– volume: 11
  start-page: 220
  year: 2006
  ident: C6AN02486F-(cit16)/*[position()=1]
  publication-title: Drug Discovery Today
  doi: 10.1016/S1359-6446(05)03735-9
  contributor:
    fullname: Forst
– volume: 44
  start-page: 701
  year: 2007
  ident: C6AN02486F-(cit8)/*[position()=1]
  publication-title: Clin. Infect. Dis.
  doi: 10.1086/509936
  contributor:
    fullname: Ruiz-Palacios
– volume: 45
  start-page: 1039
  year: 2007
  ident: C6AN02486F-(cit12)/*[position()=1]
  publication-title: Clin. Infect. Dis.
  doi: 10.1086/521911
  contributor:
    fullname: Gubler
– volume: 19
  start-page: 205
  year: 2006
  ident: C6AN02486F-(cit34)/*[position()=1]
  publication-title: Best Pract. Res., Clin. Haematol.
  doi: 10.1016/j.beha.2005.04.001
  contributor:
    fullname: Pelletier
– volume: 75
  start-page: M7
  year: 2010
  ident: C6AN02486F-(cit37)/*[position()=1]
  publication-title: J. Food Sci.
  doi: 10.1111/j.1750-3841.2009.01393.x
  contributor:
    fullname: Du
– volume: 111
  start-page: 15214
  year: 2014
  ident: C6AN02486F-(cit59)/*[position()=1]
  publication-title: Proc. Natl. Acad. Sci. U. S. A.
  doi: 10.1073/pnas.1407087111
  contributor:
    fullname: Millet
– volume: 15
  start-page: 37
  year: 2002
  ident: C6AN02486F-(cit43)/*[position()=1]
  publication-title: NMR Biomed.
  doi: 10.1002/nbm.740
  contributor:
    fullname: Belle
– volume: 2
  start-page: 19
  year: 2012
  ident: C6AN02486F-(cit35)/*[position()=1]
  publication-title: Results Immunol.
  doi: 10.1016/j.rinim.2012.01.002
  contributor:
    fullname: Dichtelmüller
– volume: 159
  start-page: 430
  year: 2012
  ident: C6AN02486F-(cit19)/*[position()=1]
  publication-title: Transl. Res.
  doi: 10.1016/j.trsl.2011.12.009
  contributor:
    fullname: Fontana
– volume: 127
  start-page: 259
  year: 2012
  ident: C6AN02486F-(cit13)/*[position()=1]
  publication-title: Public Health Rep.
  doi: 10.1177/003335491212700305
  contributor:
    fullname: Bouri
– volume: 178
  start-page: 151
  year: 2013
  ident: C6AN02486F-(cit18)/*[position()=1]
  publication-title: Virus Res.
  doi: 10.1016/j.virusres.2013.02.011
  contributor:
    fullname: Josset
– volume: 158
  start-page: 395
  year: 2002
  ident: C6AN02486F-(cit50)/*[position()=1]
  publication-title: J. Cell Biol.
  doi: 10.1083/jcb.200205044
  contributor:
    fullname: Bushell
– volume: 9
  start-page: 5748
  year: 2010
  ident: C6AN02486F-(cit49)/*[position()=1]
  publication-title: J. Proteome Res.
  doi: 10.1021/pr1005247
  contributor:
    fullname: Webb-Robertson
– volume: 38
  start-page: 65
  year: 2011
  ident: C6AN02486F-(cit36)/*[position()=1]
  publication-title: Transfus. Med. Hemother.
  doi: 10.1159/000323552
  contributor:
    fullname: Hellstern
– volume: 137
  start-page: 160
  year: 2006
  ident: C6AN02486F-(cit32)/*[position()=1]
  publication-title: J. Virol. Methods
  doi: 10.1016/j.jviromet.2006.05.027
  contributor:
    fullname: Cham
– volume: 287
  start-page: 443
  year: 2000
  ident: C6AN02486F-(cit2)/*[position()=1]
  publication-title: Science
  doi: 10.1126/science.287.5452.443
  contributor:
    fullname: Daszak
– volume: 1023
  start-page: 317
  year: 2004
  ident: C6AN02486F-(cit45)/*[position()=1]
  publication-title: J. Chromatogr., A
  doi: 10.1016/j.chroma.2003.10.029
  contributor:
    fullname: Jiang
– volume: 468
  start-page: 647
  year: 2010
  ident: C6AN02486F-(cit5)/*[position()=1]
  publication-title: Nature
  doi: 10.1038/nature09575
  contributor:
    fullname: Keesing
– volume: 9
  start-page: e1003467
  year: 2013
  ident: C6AN02486F-(cit6)/*[position()=1]
  publication-title: PLoS Pathog.
  doi: 10.1371/journal.ppat.1003467
  contributor:
    fullname: Morens
– volume: 2
  start-page: e300
  year: 2008
  ident: C6AN02486F-(cit14)/*[position()=1]
  publication-title: PLoS Neglected Trop. Dis.
  doi: 10.1371/journal.pntd.0000300
  contributor:
    fullname: Hotez
– volume: 367
  start-page: 785
  year: 2012
  ident: C6AN02486F-(cit10)/*[position()=1]
  publication-title: N. Engl. J. Med.
  doi: 10.1056/NEJMp1209051
  contributor:
    fullname: Cherry
– volume: 27
  start-page: 432
  year: 1978
  ident: C6AN02486F-(cit42)/*[position()=1]
  publication-title: J. Virol.
  doi: 10.1128/JVI.27.2.432-435.1978
  contributor:
    fullname: Fay
– volume: 347
  start-page: aaa4339
  issue: 6227
  year: 2015
  ident: C6AN02486F-(cit1)/*[position()=1]
  publication-title: Science
  doi: 10.1126/science.aaa4339
  contributor:
    fullname: Heesterbeek
– volume: 74
  start-page: 5047
  year: 2008
  ident: C6AN02486F-(cit38)/*[position()=1]
  publication-title: Appl. Environ. Microbiol.
  doi: 10.1128/AEM.00487-08
  contributor:
    fullname: Macinga
– volume: 39
  start-page: 702
  year: 2004
  ident: C6AN02486F-(cit21)/*[position()=1]
  publication-title: Clin. Infect. Dis.
  doi: 10.1086/423182
  contributor:
    fullname: Rutala
– volume: 326
  start-page: 1362
  year: 2009
  ident: C6AN02486F-(cit4)/*[position()=1]
  publication-title: Science
  doi: 10.1126/science.1177345
  contributor:
    fullname: Lloyd-Smith
– volume: 195
  start-page: 3863
  year: 2013
  ident: C6AN02486F-(cit54)/*[position()=1]
  publication-title: J. Bacteriol.
  doi: 10.1128/JB.00369-13
  contributor:
    fullname: Barra-Carrasco
– volume-title: Bergey's manual of systematic bacteriology
  year: 1984
  ident: C6AN02486F-(cit52)/*[position()=1]
  contributor:
    fullname: Bergey
– volume: 81
  start-page: 10038
  year: 2009
  ident: C6AN02486F-(cit48)/*[position()=1]
  publication-title: Anal. Chem.
  doi: 10.1021/ac9019522
  contributor:
    fullname: Kind
– volume: 77
  start-page: 204
  year: 2011
  ident: C6AN02486F-(cit22)/*[position()=1]
  publication-title: J. Hosp. Infect.
  doi: 10.1016/j.jhin.2010.06.028
  contributor:
    fullname: Maillard
– volume: 37
  start-page: 911
  year: 1959
  ident: C6AN02486F-(cit41)/*[position()=1]
  publication-title: Can. J. Biochem. Physiol.
  doi: 10.1139/y59-099
  contributor:
    fullname: Bligh
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Snippet The continued emergence and spread of infectious agents is of great concern, and systems biology approaches to infectious disease research can advance our...
The continued emergence and spread of infectious agents is of increasing concern due to increased population growth and the associated increased livestock...
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StartPage 442
SubjectTerms Bacteria
Bacteria - isolation & purification
BASIC BIOLOGICAL SCIENCES
Cell Line
Environmental Molecular Sciences Laboratory
Epithelial Cells
Exposure
Extraction
Humans
Inactivation
Lipids
Lipids - analysis
Mass Spectrometry
Membranes
Metabolomics
Pathogens
Proteins
Proteomics
Virus Inactivation
Viruses
Viruses - isolation & purification
Title MPLEx: a method for simultaneous pathogen inactivation and extraction of samples for multi-omics profiling
URI https://www.ncbi.nlm.nih.gov/pubmed/28091625
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https://www.osti.gov/biblio/1345449
https://pubmed.ncbi.nlm.nih.gov/PMC5283721
Volume 142
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