A phosphate transport system is required for symbiotic nitrogen fixation by Rhizobium meliloti

• Published in: Journal of Bacteriology Vol. 178; no. 15; pp. 4540 - 4547
• Main Authors: Bardin, S. (McMaster University, Hamilton, Ontario, Canada.), Dan, S, Osteras, M, Finan, T.M
• Format: Journal Article
• Language: English
• Published: United States American Society for Microbiology 01.08.1996
• Subjects: Amino Acid Sequence; ANION; ANIONES; ARN MENSAJERO; ARN MESSAGER; BACTERIA; Bacterial Proteins - genetics; Bacteriology; Base Sequence; Biochemistry; Biological Transport, Active - genetics; Chromosome Mapping; COMPOSICION QUIMICA; COMPOSITION CHIMIQUE; DNA Primers - genetics; DNA, Bacterial - genetics; EXPRESION GENICA; EXPRESSION DES GENES; FIJACION DEL NITROGENO; FIXATION DE L'AZOTE; FOSFATOS; GENE; GENES; Genes, Bacterial; Hordeum vulgare; ION; IONES; LOCI; LOCUS; Medicago sativa - microbiology; MEMBRANAS CELULARES; MEMBRANE CELLULAIRE; Molecular Sequence Data; MUTANT; MUTANTES; Nitrogen; Nitrogen Fixation - genetics; Nitrogen Fixation - physiology; Phenotype; PHOSPHATE; Phosphates - metabolism; PROTEINAS; PROTEINE; RHIZOBIUM MELILOTI; SECUENCIA NUCLEOTIDICA; Sequence Homology, Amino Acid; SEQUENCE NUCLEOTIDIQUE; Sinorhizobium meliloti - genetics; Sinorhizobium meliloti - metabolism; Symbiosis - genetics; Symbiosis - physiology; TRANSPORT DES SUBSTANCES NUTRITIVES; TRANSPORTE DE NUTRIENTES

The bacterium Rhizobium meliloti forms N2-fixing root nodules on alfalfa plants. The ndvF locus, located on the 1,700-kb pEXO megaplasmid of R. meliloti, is required for nodule invasion and N2 fixation. Here we report that ndvF contains four genes, phoCDET, which encode an ABC-type transport system...