Rapid bacterial identification by direct PCR amplification of 16S rRNA genes using the MinION™ nanopore sequencer

• Published in: FEBS open bio Vol. 9; no. 3; pp. 548 - 557
• Main Authors: Kai, Shinichi, Matsuo, Yoshiyuki, Nakagawa, So, Kryukov, Kirill, Matsukawa, Shino, Tanaka, Hiromasa, Iwai, Teppei, Imanishi, Tadashi, Hirota, Kiichi
• Format: Journal Article
• Language: English
• Published: England John Wiley & Sons, Inc 01.03.2019; John Wiley and Sons Inc
• Subjects: 16S rRNA; antibiotics; Bacteria; bacterial identification; Bacterial infections; Bioinformatics; Cell disruption; Cell suspensions; Cell walls; Cytology; Deoxyribonucleic acid; direct PCR; DNA; E coli; Escherichia coli - genetics; Escherichia coli - growth & development; Gene amplification; genes; Genetic engineering; Genomes; Humans; Identification; Method; MinION; nanopore sequencer; Nanopores; patients; Polymerase Chain Reaction; Purification; reaction kinetics; ribosomal RNA; RNA, Ribosomal, 16S - genetics; rRNA 16S; Sequence Analysis, RNA; Software; Staphylococcus aureus - genetics; Staphylococcus aureus - growth & development; taxonomy; Thermal cycling; United States > US; nanopore sequencer; 16S rRNA; MinION; bacterial identification; direct PCR

Rapid identification of bacterial pathogens is crucial for appropriate and adequate antibiotic treatment, which significantly improves patient outcomes. 16S ribosomal RNA (rRNA) gene amplicon sequencing has proven to be a powerful strategy for diagnosing bacterial infections. We have recently establ...