Rapid bacterial identification by direct PCR amplification of 16S rRNA genes using the MinION™ nanopore sequencer - Kai, Shinichi; Matsuo, Yoshiyuki; Nakagawa, So; Kryukov, Kirill; Matsukawa, Shino; Tanaka, Hiromasa; Iwai, Teppei; Imanishi, Tadashi; Hirota, Kiichi (FEBS open bio, 2019)
Keywords: 16S rRNA; antibiotics; Bacteria; bacterial identification; Bacterial infections; Bioinformatics; Cell disruption; Cell suspensions; Cell walls; Cytology; Deoxyribonucleic acid; direct PCR; DNA; E coli; Escherichia coli - genetics; Escherichia coli - growth & development; Gene amplification; genes; Genetic engineering; Genomes; Humans; Identification; Method; MinION; nanopore sequencer; Nanopores; patients; Polymerase Chain Reaction; Purification; reaction kinetics; ribosomal RNA; RNA, Ribosomal, 16S - genetics; rRNA 16S; Sequence Analysis, RNA; Software; Staphylococcus aureus - genetics; Staphylococcus aureus - growth & development; taxonomy; Thermal cycling; United States--US; nanopore sequencer; 16S rRNA; MinION; bacterial identification; direct PCR