高效液相色谱-三重四级杆/复合线性离子阱质谱测定鼠脑组织中神经甾体类化合物
建立了高效液相色谱-串联质谱法（HPLC-MS/MS）测定鼠脑组织中6种神经甾体类激素（别孕烯醇酮、孕烯醇酮、孕酮、脱氧皮质酮、四氢脱氧皮质酮、去氢表雄酮）的方法。采用Agilent XDB-C18色谱柱,以5 mmol/L乙酸铵-甲醇为流动相,梯度洗脱分离,在电喷雾正离子模式下,以多反应监测（MRM）扫描模式采集数据,基质匹配内标法定量。孕酮和脱氧皮质酮在0.10~10 ng/m L范围内呈良好的线性关系,别孕烯醇酮、孕烯醇酮、四氢脱氧皮质酮、去氢表雄酮在0.50~10 ng/m L范围内呈良好的线性关系,相关系数均大于0.99;在0.4,4.0和8.0 ng/m L加标水平下的平均回收率为...
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| Published in | 分析化学 Vol. 43; no. 8; pp. 1118 - 1124 |
|---|---|
| Main Author | |
| Format | Journal Article |
| Language | Chinese |
| Published |
北京大学系统生物医学研究所,北京,100191%北京大学神经科学研究所,北京,100191%中国人民解放军白求恩国际和平医院药学部,石家庄,050082
2015
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| Abstract | 建立了高效液相色谱-串联质谱法（HPLC-MS/MS）测定鼠脑组织中6种神经甾体类激素（别孕烯醇酮、孕烯醇酮、孕酮、脱氧皮质酮、四氢脱氧皮质酮、去氢表雄酮）的方法。采用Agilent XDB-C18色谱柱,以5 mmol/L乙酸铵-甲醇为流动相,梯度洗脱分离,在电喷雾正离子模式下,以多反应监测（MRM）扫描模式采集数据,基质匹配内标法定量。孕酮和脱氧皮质酮在0.10~10 ng/m L范围内呈良好的线性关系,别孕烯醇酮、孕烯醇酮、四氢脱氧皮质酮、去氢表雄酮在0.50~10 ng/m L范围内呈良好的线性关系,相关系数均大于0.99;在0.4,4.0和8.0 ng/m L加标水平下的平均回收率为91.2%~115.5%;相对标准偏差（RSD,n=6）为0.87%~8.8%;检出限为0.04~0.20 ng/m L,定量限为0.10~0.50 ng/m L。 |
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| AbstractList | 建立了高效液相色谱-串联质谱法(HPLC-MS/MS)测定鼠脑组织中6种神经甾体类激素(别孕烯醇酮、孕烯醇酮、孕酮、脱氧皮质酮、四氢脱氧皮质酮、去氢表雄酮)的方法.采用Agilent XDB-C18色谱柱,以5 mmol/L乙酸铵-甲醇为流动相,梯度洗脱分离,在电喷雾正离子模式下,以多反应监测(MRM)扫描模式采集数据,基质匹配内标法定量.孕酮和脱氧皮质酮在0.10～ 10 ng/mL范围内呈良好的线性关系,别孕烯醇酮、孕烯醇酮、四氢脱氧皮质酮、去氢表雄酮在0.50 ～ 10 ng/mL范围内呈良好的线性关系,相关系数均大于0.99;在0.4,4.0和8.0 ng/mL加标水平下的平均回收率为91.2％～115.5％;相对标准偏差(RSD,n=6)为0.87％～8.8％;检出限为0.04 ～0.20 ng/mL,定量限为0.10～0.50 ng/mL. 建立了高效液相色谱-串联质谱法（HPLC-MS/MS）测定鼠脑组织中6种神经甾体类激素（别孕烯醇酮、孕烯醇酮、孕酮、脱氧皮质酮、四氢脱氧皮质酮、去氢表雄酮）的方法。采用Agilent XDB-C18色谱柱,以5 mmol/L乙酸铵-甲醇为流动相,梯度洗脱分离,在电喷雾正离子模式下,以多反应监测（MRM）扫描模式采集数据,基质匹配内标法定量。孕酮和脱氧皮质酮在0.10~10 ng/m L范围内呈良好的线性关系,别孕烯醇酮、孕烯醇酮、四氢脱氧皮质酮、去氢表雄酮在0.50~10 ng/m L范围内呈良好的线性关系,相关系数均大于0.99;在0.4,4.0和8.0 ng/m L加标水平下的平均回收率为91.2%~115.5%;相对标准偏差（RSD,n=6）为0.87%~8.8%;检出限为0.04~0.20 ng/m L,定量限为0.10~0.50 ng/m L。 |
| Author | 刘佳 张朦 万有 吴红海 侯艳宁 郑乐民 尹玉新 |
| AuthorAffiliation | 北京大学系统生物医学研究所,北京100191 北京大学神经科学研究所,北京100191 中国人民解放军白求恩国际和平医院药学部,石家庄050082 |
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| Author_FL | LIU Jia WU Hong-Hai ZHENG Le-Min ZHANG Meng WAN You YIN Yu-Xin HOU Yan-Ning |
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| DocumentTitleAlternate | Simultaneous Determination of Six Neurosteroids Hormones in Rat Brain Tissues by High Performance Liquid Chromatography-Tandem Mass Spectrometry |
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| Keywords | 神经甾体 鼠脑 液相串联质谱 多重反应监测 Neurosteroids Multiple reaction monitoring Liquid chromatography-tandem mass spectrometry Rat brain |
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| Notes | LIU Jia, ZHANG Meng, WAN You, WU Hong-Hai, HOU Yan-Ning, ZHENG Le-Min, YIN Yu-Xin 1（ Institute of Systems Biomedicine, Peking University Health Science Center, Beijing 100191, China） 2 （ Neurosctence Research Institute, Peking University, Beijing 100191, China） 3 （ Bethune International Peace Hospital, Shijiazhuang 050082, China） 22-1125/O6 Neurosteroids; Liquid chromatography-tandem mass spectrometry; Multiple reaction monitoring; Rat brain An analytical method based on high performance liquid chromatography tandem mass spectrometry was developed for the simultaneous determination of 6 neurosteroids in rat brain tissues. The neurosteroids were separated on an Agilent XDB-Cls column （50 mm×4.6 mm, 1.8 μm）, using methanol-5 mmol/L ammonium acetate as the mobile phase. A gradient elution program with a cycle time of 10 min was used. The neurosteroids were detected by electrospray ionization mass spectrometry in positive mode with multiple reaction monitoring （MRM） scan mode. For ll-deoxycorticosterone （DOC） and pr |
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| PublicationTitle | 分析化学 |
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| PublicationYear | 2015 |
| Publisher | 北京大学系统生物医学研究所,北京,100191%北京大学神经科学研究所,北京,100191%中国人民解放军白求恩国际和平医院药学部,石家庄,050082 |
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| Snippet | 建立了高效液相色谱-串联质谱法（HPLC-MS/MS）测定鼠脑组织中6种神经甾体类激素（别孕烯醇酮、孕烯醇酮、孕酮、脱氧皮质酮、四氢脱氧皮质酮、去氢表雄酮）的方法。采用Agilent XDB-C18色谱柱,以5... 建立了高效液相色谱-串联质谱法(HPLC-MS/MS)测定鼠脑组织中6种神经甾体类激素(别孕烯醇酮、孕烯醇酮、孕酮、脱氧皮质酮、四氢脱氧皮质酮、去氢表雄酮)的方法.采用Agilent XDB-C18色谱柱,以5... |
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| SubjectTerms | 多重反应监测 液相串联质谱 神经甾体 鼠脑 |
| Title | 高效液相色谱-三重四级杆/复合线性离子阱质谱测定鼠脑组织中神经甾体类化合物 |
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