DNA methylation and chromatin accessibility profiling of mouse and human fetal germ cells

• Published in: Cell research Vol. 27; no. 2; pp. 165 - 183
• Main Authors: Guo, Hongshan, Hu, Boqiang, Yan, Liying, Yong, Jun, Wu, Yan, Gao, Yun, Guo, Fan, Hou, Yu, Fan, Xiaoying, Dong, Ji, Wang, Xiaoye, Zhu, Xiaohui, Yan, Jie, Wei, Yuan, Jin, Hongyan, Zhang, Wenxin, Wen, Lu, Tang, Fuchou, Qiao, Jie
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 01.02.2017; Nature Publishing Group
• Subjects: 631/136/532/2435; 631/208/176/1988; 631/337/100; 692/698/690/1520; Animals; Biomedical and Life Sciences; Cell Biology; Cellular Reprogramming - genetics; Chromatin - metabolism; Deoxyribonucleic acid; DNA; DNA Methylation - genetics; DNA甲基化; Fetus - cytology; Genomic Imprinting; Germ Cells - metabolism; Humans; Life Sciences; Male; Mammals - genetics; Mice; Nucleosomes - metabolism; Organ Specificity - genetics; Original; original-article; Promoter Regions, Genetic - genetics; Repetitive Sequences, Nucleic Acid - genetics; 原始生殖细胞; 可及性; 小鼠; 染色质重塑; 生殖细胞发育; 胚胎生殖细胞; 表观遗传; development; transcription; chromatin; stem cell biology; epigenetics

Chromatin remodeling is important for the epigenetic reprogramming of human primordial germ cells. However, the comprehensive chromatin state has not yet been analyzed for human fetal germ ceils （FGCs）. Here we use nucleosome occupancy and methylation sequencing method to analyze both the genome-wid...