DNA methylation and chromatin accessibility profiling of mouse and human fetal germ cells
Chromatin remodeling is important for the epigenetic reprogramming of human primordial germ cells. However, the comprehensive chromatin state has not yet been analyzed for human fetal germ ceils （FGCs）. Here we use nucleosome occupancy and methylation sequencing method to analyze both the genome-wid...
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| Published in | Cell research Vol. 27; no. 2; pp. 165 - 183 |
|---|---|
| Main Authors | , , , , , , , , , , , , , , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
London
Nature Publishing Group UK
01.02.2017
Nature Publishing Group |
| Subjects | |
| Online Access | Get full text |
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| Abstract | Chromatin remodeling is important for the epigenetic reprogramming of human primordial germ cells. However, the comprehensive chromatin state has not yet been analyzed for human fetal germ ceils （FGCs）. Here we use nucleosome occupancy and methylation sequencing method to analyze both the genome-wide chromatin accessibility and DNA methylome at a series of crucial time points during fetal germ cell development in both human and mouse. We find 116 887 and 137 557 nucleosome-depleted regions （NDRs） in human and mouse FGCs, covering a large set of germline-specific and highly dynamic regulatory genomic elements, such as enhancers. Moreover, we find that the distal NDRs are enriched specifically for binding motifs of the pluripotency and germ cell master regulators such as NANOG, SOX17, AP2γ and OCT4 in human FGCs, indicating the existence of a delicate regulatory balance between pluripotency-related genes and germ cell-specific genes in human FGCs, and the functional significance of these genes for germ cell development in vivo. Our work offers a comprehensive and high-resolution roadmap for dissecting chromatin state transition dynamics during the epigenomic reprogramming of human and mouse FGCs. |
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| AbstractList | Chromatin remodeling is important for the epigenetic reprogramming of human primordial germ cells. However, the comprehensive chromatin state has not yet been analyzed for human fetal germ cells (FGCs). Here we use nucleosome occupancy and methylation sequencing method to analyze both the genome-wide chromatin accessibility and DNA methylome at a series of crucial time points during fetal germ cell development in both human and mouse. We find 116 887 and 137 557 nucleosome-depleted regions (NDRs) in human and mouse FGCs, covering a large set of germline-specific and highly dynamic regulatory genomic elements, such as enhancers. Moreover, we find that the distal NDRs are enriched specifically for binding motifs of the pluripotency and germ cell master regulators such as NANOG, SOX17, AP2γ and OCT4 in human FGCs, indicating the existence of a delicate regulatory balance between pluripotency-related genes and germ cell-specific genes in human FGCs, and the functional significance of these genes for germ cell development in vivo. Our work offers a comprehensive and high-resolution roadmap for dissecting chromatin state transition dynamics during the epigenomic reprogramming of human and mouse FGCs. Chromatin remodeling is important for the epigenetic reprogramming of human primordial germ cells. However, the comprehensive chromatin state has not yet been analyzed for human fetal germ cells (FGCs). Here we use nucleosome occupancy and methylation sequencing method to analyze both the genome-wide chromatin accessibility and DNA methylome at a series of crucial time points during fetal germ cell development in both human and mouse. We find 116 887 and 137 557 nucleosome-depleted regions (NDRs) in human and mouse FGCs, covering a large set of germline-specific and highly dynamic regulatory genomic elements, such as enhancers. Moreover, we find that the distal NDRs are enriched specifically for binding motifs of the pluripotency and germ cell master regulators such as NANOG, SOX17, AP2γ and OCT4 in human FGCs, indicating the existence of a delicate regulatory balance between pluripotency-related genes and germ cell-specific genes in human FGCs, and the functional significance of these genes for germ cell development in vivo. Our work offers a comprehensive and high-resolution roadmap for dissecting chromatin state transition dynamics during the epigenomic reprogramming of human and mouse FGCs.Chromatin remodeling is important for the epigenetic reprogramming of human primordial germ cells. However, the comprehensive chromatin state has not yet been analyzed for human fetal germ cells (FGCs). Here we use nucleosome occupancy and methylation sequencing method to analyze both the genome-wide chromatin accessibility and DNA methylome at a series of crucial time points during fetal germ cell development in both human and mouse. We find 116 887 and 137 557 nucleosome-depleted regions (NDRs) in human and mouse FGCs, covering a large set of germline-specific and highly dynamic regulatory genomic elements, such as enhancers. Moreover, we find that the distal NDRs are enriched specifically for binding motifs of the pluripotency and germ cell master regulators such as NANOG, SOX17, AP2γ and OCT4 in human FGCs, indicating the existence of a delicate regulatory balance between pluripotency-related genes and germ cell-specific genes in human FGCs, and the functional significance of these genes for germ cell development in vivo. Our work offers a comprehensive and high-resolution roadmap for dissecting chromatin state transition dynamics during the epigenomic reprogramming of human and mouse FGCs. Chromatin remodeling is important for the epigenetic reprogramming of human primordial germ cells. However, the comprehensive chromatin state has not yet been analyzed for human fetal germ cells (FGCs). Here we use nucleosome occupancy and methylation sequencing method to analyze both the genome-wide chromatin accessibility and DNA methylome at a series of crucial time points during fetal germ cell development in both human and mouse. We find 116 887 and 137 557 nucleosome-depleted regions (NDRs) in human and mouse FGCs, covering a large set of germline-specific and highly dynamic regulatory genomic elements, such as enhancers. Moreover, we find that the distal NDRs are enriched specifically for binding motifs of the pluripotency and germ cell master regulators such as NANOG, SOX17, AP2γ and OCT4 in human FGCs, indicating the existence of a delicate regulatory balance between pluripotency-related genes and germ cell-specific genes in human FGCs, and the functional significance of these genes for germ cell development in vivo . Our work offers a comprehensive and high-resolution roadmap for dissecting chromatin state transition dynamics during the epigenomic reprogramming of human and mouse FGCs. Chromatin remodeling is important for the epigenetic reprogramming of human primordial germ cells. However, the comprehensive chromatin state has not yet been analyzed for human fetal germ ceils （FGCs）. Here we use nucleosome occupancy and methylation sequencing method to analyze both the genome-wide chromatin accessibility and DNA methylome at a series of crucial time points during fetal germ cell development in both human and mouse. We find 116 887 and 137 557 nucleosome-depleted regions （NDRs） in human and mouse FGCs, covering a large set of germline-specific and highly dynamic regulatory genomic elements, such as enhancers. Moreover, we find that the distal NDRs are enriched specifically for binding motifs of the pluripotency and germ cell master regulators such as NANOG, SOX17, AP2γ and OCT4 in human FGCs, indicating the existence of a delicate regulatory balance between pluripotency-related genes and germ cell-specific genes in human FGCs, and the functional significance of these genes for germ cell development in vivo. Our work offers a comprehensive and high-resolution roadmap for dissecting chromatin state transition dynamics during the epigenomic reprogramming of human and mouse FGCs. Chromatin remodeling is important for the epigenetic reprogramming of human primordial germ cells. However, the comprehensive chromatin state has not yet been analyzed for human fetal germ cells (FGCs). Here we use nucleosome occupancy and methylation sequencing method to analyze both the genome-wide chromatin accessibility and DNA methylome at a series of crucial time points during fetal germ cell development in both human and mouse. We find 116 887 and 137 557 nucleosome-depleted regions (NDRs) in human and mouse FGCs, covering a large set of germline-specific and highly dynamic regulatory genomic elements, such as enhancers. Moreover, we find that the distal NDRs are enriched specifically for binding motifs of the pluripotency and germ cell master regulators such as NANOG, SOX17, AP2 gamma and OCT4 in human FGCs, indicating the existence of a delicate regulatory balance between pluripotency-related genes and germ cell-specific genes in human FGCs, and the functional significance of these genes for germ cell development in vivo. Our work offers a comprehensive and high-resolution roadmap for dissecting chromatin state transition dynamics during the epigenomic reprogramming of human and mouse FGCs. |
| Author | Hongshan Guo Boqiang Hu Liying Yan Jun Yong Yan Wu Yun Gao Fan Guo Yu Hou Xiaoying Fan Ji Dong Xiaoye Wang Xiaohui Zhu Jie Yan Yuan Wei Hongyan Jin Wenxin Zhang Lu Wen FuchouTang Jie Qiao |
| AuthorAffiliation | Department of Obstetrics and Gynecology Third Hospital, Biomedical Institute for Pioneering Investigation via Convergence ＆ Center for Reproductive Medicine, College of Life Sciences, Peking University, Beijing 100871, China Key Laboratory of Assisted Reproduction, Ministry of Education, Beijing 100191, China Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproductive Technology, Beijing100191, China National Institute of Biological Sciences, Beijing 102206, China Ministry of Education Key Laboratory of Cell Proliferation and Differentiation, Beijing 100871, China Beijing Advanced Innovation Center for Genomics, Beijing 100871, China peking-Tsinghua Center for Life Sciences, Peking University, Beijing 100871, China |
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Institute for Pioneering Investigation via Convergence & Center for Reproductive Medicine, College of Life Sciences, Peking University, Key Laboratory of Assisted Reproduction, Ministry of Education, Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproductive Technology – sequence: 14 givenname: Yuan surname: Wei fullname: Wei, Yuan organization: Department of Obstetrics and Gynecology Third Hospital, Biomedical Institute for Pioneering Investigation via Convergence & Center for Reproductive Medicine, College of Life Sciences, Peking University, Key Laboratory of Assisted Reproduction, Ministry of Education, Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproductive Technology – sequence: 15 givenname: Hongyan surname: Jin fullname: Jin, Hongyan organization: Department of Obstetrics and Gynecology Third Hospital, Biomedical Institute for Pioneering Investigation via Convergence & Center for Reproductive Medicine, College of Life Sciences, Peking University, Key Laboratory of Assisted Reproduction, Ministry of Education, Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproductive Technology – sequence: 16 givenname: Wenxin surname: Zhang fullname: Zhang, Wenxin organization: Department of Obstetrics and Gynecology Third Hospital, Biomedical Institute for Pioneering Investigation via Convergence & Center for Reproductive Medicine, College of Life Sciences, Peking University, Key Laboratory of Assisted Reproduction, Ministry of Education, Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproductive Technology – sequence: 17 givenname: Lu surname: Wen fullname: Wen, Lu organization: Department of Obstetrics and Gynecology Third Hospital, Biomedical Institute for Pioneering Investigation via Convergence & Center for Reproductive Medicine, College of Life Sciences, Peking University, Ministry of Education Key Laboratory of Cell Proliferation and Differentiation – sequence: 18 givenname: Fuchou surname: Tang fullname: Tang, Fuchou email: tangfuchou@pku.edu.cn organization: Department of Obstetrics and Gynecology Third Hospital, Biomedical Institute for Pioneering Investigation via Convergence & Center for Reproductive Medicine, College of Life Sciences, Peking University, Ministry of Education Key Laboratory of Cell Proliferation and Differentiation, Beijing Advanced Innovation Center for Genomics, Peking-Tsinghua Center for Life Sciences, Peking University – sequence: 19 givenname: Jie surname: Qiao fullname: Qiao, Jie email: jie.qiao@263.net organization: Department of Obstetrics and Gynecology Third Hospital, Biomedical Institute for Pioneering Investigation via Convergence & Center for Reproductive Medicine, College of Life Sciences, Peking University, Key Laboratory of Assisted Reproduction, Ministry of Education, Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproductive Technology, Peking-Tsinghua Center for Life Sciences, Peking University |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/27824029$$D View this record in MEDLINE/PubMed |
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| Cites_doi | 10.1016/j.devcel.2012.12.011 10.1101/gr.161679.113 10.1038/nature09147 10.1242/dev.050849 10.1126/science.1226889 10.1093/bioinformatics/btl567 10.1126/science.aab1601 10.1242/dev.005611 10.1038/nmeth.3035 10.1016/j.ydbio.2004.11.025 10.1016/j.cell.2007.12.014 10.1016/j.celrep.2013.04.032 10.1038/nature07829 10.1016/j.cell.2015.04.053 10.1038/nature10002 10.1016/j.stem.2014.05.008 10.1073/pnas.1315204110 10.1016/j.cell.2008.02.022 10.1038/nature14590 10.1016/j.cell.2014.12.013 10.1038/nmeth.2688 10.1016/j.stem.2016.05.001 10.1101/gad.947102 10.1016/j.molcel.2010.05.004 10.1093/nar/gks454 10.1016/j.cell.2015.05.015 10.1095/biolreprod.107.066175 10.1038/nature13544 10.1038/nature16480 10.1128/MCB.05276-11 10.1038/nprot.2012.016 10.1101/gr.163485.113 10.1038/nmeth.1315 10.1038/nmeth.3252 10.1371/journal.pgen.1004868 10.1101/gr.148023.112 10.1093/molehr/7.9.845 10.1074/jbc.M115.672931 10.1038/nprot.2009.236 10.1016/j.stem.2016.01.017 10.1093/bioinformatics/btp352 10.1016/j.cell.2013.04.044 10.1016/j.cell.2015.05.012 10.1016/j.molcel.2012.11.001 10.1530/REP-13-0069 10.1101/gr.188516.114 10.1371/journal.pcbi.1003354 10.1101/gr.157750.113 10.1038/nature11243 10.1038/nature13581 10.1016/j.stem.2015.03.002 10.1186/gb-2009-10-3-r25 10.1186/gb-2004-5-10-r80 10.1016/j.cell.2011.06.052 10.1038/nature12417 10.1101/gr.143008.112 10.1038/nature15740 |
| ContentType | Journal Article |
| Copyright | The Author(s) 2016 Copyright Nature Publishing Group Feb 2017 Copyright © 2016 Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 2016 Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences |
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| DocumentTitleAlternate | DNA methylation and chromatin accessibility profiling of mouse and human fetal germ cells Chromatin accessibility and DNA methylome of mammalian PGCs |
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| ISSN | 1001-0602 1748-7838 |
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| Issue | 2 |
| Keywords | development transcription chromatin stem cell biology epigenetics |
| Language | English |
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| Notes | Chromatin remodeling is important for the epigenetic reprogramming of human primordial germ cells. However, the comprehensive chromatin state has not yet been analyzed for human fetal germ ceils （FGCs）. Here we use nucleosome occupancy and methylation sequencing method to analyze both the genome-wide chromatin accessibility and DNA methylome at a series of crucial time points during fetal germ cell development in both human and mouse. We find 116 887 and 137 557 nucleosome-depleted regions （NDRs） in human and mouse FGCs, covering a large set of germline-specific and highly dynamic regulatory genomic elements, such as enhancers. Moreover, we find that the distal NDRs are enriched specifically for binding motifs of the pluripotency and germ cell master regulators such as NANOG, SOX17, AP2γ and OCT4 in human FGCs, indicating the existence of a delicate regulatory balance between pluripotency-related genes and germ cell-specific genes in human FGCs, and the functional significance of these genes for germ cell development in vivo. Our work offers a comprehensive and high-resolution roadmap for dissecting chromatin state transition dynamics during the epigenomic reprogramming of human and mouse FGCs. chromatin; epigenetics; transcription; stem cell biology; development 31-1568 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23 These four authors contributed equally to this work. |
| OpenAccessLink | https://www.nature.com/articles/cr.2016.128 |
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| PublicationTitle | Cell research |
| PublicationTitleAbbrev | Cell Res |
| PublicationTitleAlternate | Cell Research |
| PublicationYear | 2017 |
| Publisher | Nature Publishing Group UK Nature Publishing Group |
| Publisher_xml | – name: Nature Publishing Group UK – name: Nature Publishing Group |
| References | Zhou, Wang, Yuan (CR14) 2016; 18 Kurimoto, Yabuta, Hayashi (CR16) 2015; 16 Jin, Tang, Wan (CR41) 2015; 528 Yan, Guo, Hu (CR35) 2016; 291 Boyle, Davis, Shulha (CR27) 2008; 132 Heintzman, Hon, Hawkins (CR29) 2009; 459 Tang, Barbacioru, Nordman (CR49) 2010; 5 Saitou, Kagiwada, Kurimoto (CR2) 2012; 139 Murakami, Gunesdogan, Zylicz (CR30) 2016; 529 Cusanovich, Daza, Adey (CR40) 2015; 348 Seki, Yamaji, Yabuta (CR18) 2007; 134 Kobayashi, Sakurai, Miura (CR21) 2013; 23 Hackett, Surani (CR4) 2013; 153 Trapnell, Roberts, Goff (CR57) 2012; 7 Seisenberger, Andrews, Krueger (CR22) 2012; 48 Langmead, Trapnell, Pop, Salzberg (CR56) 2009; 10 Falcon, Gentleman (CR55) 2007; 23 Guo, Zhu, Yan (CR33) 2014; 511 Nakaki, Hayashi, Ohta, Kurimoto, Yabuta, Saitou (CR15) 2013; 501 Guo, Yan, Guo (CR5) 2015; 161 Schones, Cui, Cuddapah (CR25) 2008; 132 Hayashi, Ogushi, Kurimoto, Shimamoto, Ohta, Saitou (CR12) 2012; 338 De Felici, Barrios (CR43) 2013; 146 Beh, Muller, Muir, Kaplan, Landweber (CR24) 2015; 25 Saitou, Miyauchi (CR11) 2016; 18 Portella, Battistini, Orozco (CR37) 2013; 9 Buenrostro, Wu, Litzenburger (CR39) 2015; 523 Perrett, Turnpenny, Eckert (CR32) 2008; 78 Buenrostro, Giresi, Zaba, Chang, Greenleaf (CR42) 2013; 10 Smallwood, Lee, Angermueller (CR47) 2014; 11 Kelly, Liu, Lay, Liang, Berman, Jones (CR19) 2012; 22 Ng, Kumar, Muratani (CR10) 2013; 24 Taberlay, Statham, Kelly, Clark, Jones (CR20) 2014; 24 Valouev, Johnson, Boyd, Smith, Fire, Sidow (CR38) 2011; 474 Chodavarapu, Feng, Bernatavichute (CR36) 2010; 466 Guo, Zhu, Wu, Li, Wen, Tang (CR44) 2013; 23 Miura, Enomoto, Dairiki, Ito (CR46) 2012; 40 Heinz, Benner, Spann (CR52) 2010; 38 Bird (CR3) 2002; 16 Gkountela, Zhang, Shafiq (CR6) 2015; 161 Tsui, Dubuis, Gebbia (CR26) 2011; 31 Okae, Chiba, Hiura (CR51) 2014; 10 Seki, Hayashi, Itoh, Mizugaki, Saitou, Matsui (CR17) 2005; 278 Li, Handsaker, Wysoker (CR50) 2009; 25 Tang, Barbacioru, Wang (CR48) 2009; 6 Robinson, Gaskell, Saunders, Anderson (CR45) 2001; 7 Huber, Carey, Gentleman (CR53) 2015; 12 Irie, Weinberger, Tang (CR31) 2015; 160 Hayashi, Ohta, Kurimoto, Aramaki, Saitou (CR13) 2011; 146 Lee, Hore, Reik (CR1) 2014; 14 Smith, Chan, Humm (CR34) 2014; 511 Sachs, Onodera, Blaschke, Ebata, Song, Ramalho-Santos (CR9) 2013; 3 Tang, Dietmann, Irie (CR7) 2015; 161 Zhang, Vastenhouw, Feng (CR23) 2014; 24 Gentleman, Carey, Bates (CR54) 2004; 5 Shen, Yue, McCleary (CR28) 2012; 488 Lesch, Dokshin, Young, McCarrey, Page (CR8) 2013; 110 AP Boyle (BFcr2016128_CR27) 2008; 132 M Sachs (BFcr2016128_CR9) 2013; 3 H Kobayashi (BFcr2016128_CR21) 2013; 23 SA Smallwood (BFcr2016128_CR47) 2014; 11 C Trapnell (BFcr2016128_CR57) 2012; 7 A Bird (BFcr2016128_CR3) 2002; 16 W Jin (BFcr2016128_CR41) 2015; 528 F Nakaki (BFcr2016128_CR15) 2013; 501 Y Zhang (BFcr2016128_CR23) 2014; 24 BJ Lesch (BFcr2016128_CR8) 2013; 110 S Heinz (BFcr2016128_CR52) 2010; 38 RK Chodavarapu (BFcr2016128_CR36) 2010; 466 DE Schones (BFcr2016128_CR25) 2008; 132 JD Buenrostro (BFcr2016128_CR39) 2015; 523 JH Ng (BFcr2016128_CR10) 2013; 24 H Guo (BFcr2016128_CR44) 2013; 23 M Saitou (BFcr2016128_CR2) 2012; 139 S Falcon (BFcr2016128_CR55) 2007; 23 Q Zhou (BFcr2016128_CR14) 2016; 18 Y Shen (BFcr2016128_CR28) 2012; 488 JD Buenrostro (BFcr2016128_CR42) 2013; 10 DA Cusanovich (BFcr2016128_CR40) 2015; 348 WW Tang (BFcr2016128_CR7) 2015; 161 A Valouev (BFcr2016128_CR38) 2011; 474 Y Seki (BFcr2016128_CR17) 2005; 278 K Hayashi (BFcr2016128_CR12) 2012; 338 F Miura (BFcr2016128_CR46) 2012; 40 F Tang (BFcr2016128_CR49) 2010; 5 H Okae (BFcr2016128_CR51) 2014; 10 M De Felici (BFcr2016128_CR43) 2013; 146 JA Hackett (BFcr2016128_CR4) 2013; 153 Y Seki (BFcr2016128_CR18) 2007; 134 K Tsui (BFcr2016128_CR26) 2011; 31 K Kurimoto (BFcr2016128_CR16) 2015; 16 G Portella (BFcr2016128_CR37) 2013; 9 TK Kelly (BFcr2016128_CR19) 2012; 22 PC Taberlay (BFcr2016128_CR20) 2014; 24 H Guo (BFcr2016128_CR33) 2014; 511 H Li (BFcr2016128_CR50) 2009; 25 M Saitou (BFcr2016128_CR11) 2016; 18 N Irie (BFcr2016128_CR31) 2015; 160 F Tang (BFcr2016128_CR48) 2009; 6 HJ Lee (BFcr2016128_CR1) 2014; 14 S Gkountela (BFcr2016128_CR6) 2015; 161 LY Beh (BFcr2016128_CR24) 2015; 25 L Yan (BFcr2016128_CR35) 2016; 291 ND Heintzman (BFcr2016128_CR29) 2009; 459 K Hayashi (BFcr2016128_CR13) 2011; 146 RC Gentleman (BFcr2016128_CR54) 2004; 5 K Murakami (BFcr2016128_CR30) 2016; 529 RM Perrett (BFcr2016128_CR32) 2008; 78 ZD Smith (BFcr2016128_CR34) 2014; 511 LL Robinson (BFcr2016128_CR45) 2001; 7 W Huber (BFcr2016128_CR53) 2015; 12 F Guo (BFcr2016128_CR5) 2015; 161 B Langmead (BFcr2016128_CR56) 2009; 10 S Seisenberger (BFcr2016128_CR22) 2012; 48 22960375 - Genome Res. 2012 Dec;22(12):2497-506 23727241 - Cell Rep. 2013 Jun 27;3(6):1777-84 24916973 - Genome Res. 2014 Sep;24(9):1421-32 22147951 - Development. 2012 Jan;139(1):15-31 22383036 - Nat Protoc. 2012 Mar 01;7(3):562-78 23042295 - Science. 2012 Nov 16;338(6109):971-5 15680362 - Dev Biol. 2005 Feb 15;278(2):440-58 17567665 - Development. 2007 Jul;134(14):2627-38 22649061 - Nucleic Acids Res. 2012 Sep 1;40(17):e136 22763441 - Nature. 2012 Aug 2;488(7409):116-20 26923202 - Cell Stem Cell. 2016 Mar 3;18(3):330-40 26330564 - Genome Res. 2015 Nov;25(11):1727-38 18243105 - Cell. 2008 Jan 25;132(2):311-22 21820164 - Cell. 2011 Aug 19;146(4):519-32 25042786 - Nat Methods. 2014 Aug;11(8):817-20 25800778 - Cell Stem Cell. 2015 May 7;16(5):517-32 26004067 - Cell. 2015 Jun 4;161(6):1425-36 20512117 - Nature. 2010 Jul 15;466(7304):388-92 25633503 - Nat Methods. 2015 Feb;12(2):115-21 26605532 - Nature. 2015 Dec 3;528(7580):142-6 26046443 - Cell. 2015 Jun 4;161(6):1437-52 18199879 - Biol Reprod. 2008 May;78(5):852-8 19505943 - Bioinformatics. 2009 Aug 15;25(16):2078-9 11517291 - Mol Hum Reprod. 2001 Sep;7(9):845-52 25543152 - Cell. 2015 Jan 15;160(1-2):253-68 17098774 - Bioinformatics. 2007 Jan 15;23(2):257-8 24097267 - Nat Methods. 2013 Dec;10(12):1213-8 23352811 - Dev Cell. 2013 Feb 11;24(3):324-33 26719341 - J Biol Chem. 2016 Feb 26;291(9):4386-98 21896781 - Mol Cell Biol. 2011 Nov;31(21):4348-55 23410886 - Genome Res. 2013 Apr;23(4):616-27 25953818 - Science. 2015 May 22;348(6237):910-4 24905162 - Cell Stem Cell. 2014 Jun 5;14(6):710-9 24043772 - Proc Natl Acad Sci U S A. 2013 Oct 1;110(40):16061-6 27257761 - Cell Stem Cell. 2016 Jun 2;18(6):721-35 24179143 - Genome Res. 2013 Dec;23(12):2126-35 20203668 - Nat Protoc. 2010 Mar;5(3):516-35 23663772 - Cell. 2013 May 9;153(4):737-9 26046444 - Cell. 2015 Jun 4;161(6):1453-67 11782440 - Genes Dev. 2002 Jan 1;16(1):6-21 21602827 - Nature. 2011 May 22;474(7352):516-20 20513432 - Mol Cell. 2010 May 28;38(4):576-89 26083756 - Nature. 2015 Jul 23;523(7561):486-90 15461798 - Genome Biol. 2004;5(10):R80 19261174 - Genome Biol. 2009;10(3):R25 25501653 - PLoS Genet. 2014 Dec 11;10(12):e1004868 18329373 - Cell. 2008 Mar 7;132(5):887-98 25079558 - Nature. 2014 Jul 31;511(7511):611-5 23801781 - Reproduction. 2013 Aug 12;146(4):R125-30 24278005 - PLoS Comput Biol. 2013;9(11):e1003354 19295514 - Nature. 2009 May 7;459(7243):108-12 19349980 - Nat Methods. 2009 May;6(5):377-82 23219530 - Mol Cell. 2012 Dec 28;48(6):849-62 23913270 - Nature. 2013 Sep 12;501(7466):222-6 24285721 - Genome Res. 2014 Feb;24(2):260-6 25079557 - Nature. 2014 Jul 31;511(7511):606-10 26751055 - Nature. 2016 Jan 21;529(7586):403-7 |
| References_xml | – volume: 24 start-page: 324 year: 2013 end-page: 333 ident: CR10 article-title: epigenomic profiling of germ cells reveals germ cell molecular signatures publication-title: Dev Cell doi: 10.1016/j.devcel.2012.12.011 – volume: 23 start-page: 2126 year: 2013 end-page: 2135 ident: CR44 article-title: Single-cell methylome landscapes of mouse embryonic stem cells and early embryos analyzed using reduced representation bisulfite sequencing publication-title: Genome Res doi: 10.1101/gr.161679.113 – volume: 466 start-page: 388 year: 2010 end-page: 392 ident: CR36 article-title: Relationship between nucleosome positioning and DNA methylation publication-title: Nature doi: 10.1038/nature09147 – volume: 139 start-page: 15 year: 2012 end-page: 31 ident: CR2 article-title: Epigenetic reprogramming in mouse pre-implantation development and primordial germ cells publication-title: Development doi: 10.1242/dev.050849 – volume: 338 start-page: 971 year: 2012 end-page: 975 ident: CR12 article-title: Offspring from oocytes derived from primordial germ cell-like cells in mice publication-title: Science doi: 10.1126/science.1226889 – volume: 23 start-page: 257 year: 2007 end-page: 258 ident: CR55 article-title: Using GOstats to test gene lists for GO term association publication-title: Bioinformatics doi: 10.1093/bioinformatics/btl567 – volume: 348 start-page: 910 year: 2015 end-page: 914 ident: CR40 article-title: Multiplex single cell profiling of chromatin accessibility by combinatorial cellular indexing publication-title: Science doi: 10.1126/science.aab1601 – volume: 134 start-page: 2627 year: 2007 end-page: 2638 ident: CR18 article-title: Cellular dynamics associated with the genome-wide epigenetic reprogramming in migrating primordial germ cells in mice publication-title: Development doi: 10.1242/dev.005611 – volume: 11 start-page: 817 year: 2014 end-page: 820 ident: CR47 article-title: Single-cell genome-wide bisulfite sequencing for assessing epigenetic heterogeneity publication-title: Nat Methods doi: 10.1038/nmeth.3035 – volume: 278 start-page: 440 year: 2005 end-page: 458 ident: CR17 article-title: Extensive and orderly reprogramming of genome-wide chromatin modifications associated with specification and early development of germ cells in mice publication-title: Dev Biol doi: 10.1016/j.ydbio.2004.11.025 – volume: 132 start-page: 311 year: 2008 end-page: 322 ident: CR27 article-title: High-resolution mapping and characterization of open chromatin across the genome publication-title: Cell doi: 10.1016/j.cell.2007.12.014 – volume: 3 start-page: 1777 year: 2013 end-page: 1784 ident: CR9 article-title: Bivalent chromatin marks developmental regulatory genes in the mouse embryonic germline publication-title: Cell Rep doi: 10.1016/j.celrep.2013.04.032 – volume: 459 start-page: 108 year: 2009 end-page: 112 ident: CR29 article-title: Histone modifications at human enhancers reflect global cell-type-specific gene expression publication-title: Nature doi: 10.1038/nature07829 – volume: 161 start-page: 1453 year: 2015 end-page: 1467 ident: CR7 article-title: A unique gene regulatory network resets the human germline epigenome for development publication-title: Cell doi: 10.1016/j.cell.2015.04.053 – volume: 474 start-page: 516 year: 2011 end-page: 520 ident: CR38 article-title: Determinants of nucleosome organization in primary human cells publication-title: Nature doi: 10.1038/nature10002 – volume: 14 start-page: 710 year: 2014 end-page: 719 ident: CR1 article-title: Reprogramming the methylome: erasing memory and creating diversity publication-title: Cell Stem Cell doi: 10.1016/j.stem.2014.05.008 – volume: 110 start-page: 16061 year: 2013 end-page: 16066 ident: CR8 article-title: A set of genes critical to development is epigenetically poised in mouse germ cells from fetal stages through completion of meiosis publication-title: Proc Natl Acad Sci USA doi: 10.1073/pnas.1315204110 – volume: 132 start-page: 887 year: 2008 end-page: 898 ident: CR25 article-title: Dynamic regulation of nucleosome positioning in the human genome publication-title: Cell doi: 10.1016/j.cell.2008.02.022 – volume: 523 start-page: 486 year: 2015 end-page: 490 ident: CR39 article-title: Single-cell chromatin accessibility reveals principles of regulatory variation publication-title: Nature doi: 10.1038/nature14590 – volume: 160 start-page: 253 year: 2015 end-page: 268 ident: CR31 article-title: SOX17 is a critical specifier of human primordial germ cell fate publication-title: Cell doi: 10.1016/j.cell.2014.12.013 – volume: 10 start-page: 1213 year: 2013 end-page: 1218 ident: CR42 article-title: Transposition of native chromatin for fast and sensitive epigenomic profiling of open chromatin, DNA-binding proteins and nucleosome position publication-title: Nat Methods doi: 10.1038/nmeth.2688 – volume: 18 start-page: 721 year: 2016 end-page: 735 ident: CR11 article-title: Gametogenesis from pluripotent stem cells publication-title: Cell Stem Cell doi: 10.1016/j.stem.2016.05.001 – volume: 16 start-page: 6 year: 2002 end-page: 21 ident: CR3 article-title: DNA methylation patterns and epigenetic memory publication-title: Genes Dev doi: 10.1101/gad.947102 – volume: 38 start-page: 576 year: 2010 end-page: 589 ident: CR52 article-title: Simple combinations of lineage-determining transcription factors prime cis-regulatory elements required for macrophage and B cell identities publication-title: Mol Cell doi: 10.1016/j.molcel.2010.05.004 – volume: 40 start-page: e136 year: 2012 ident: CR46 article-title: Amplification-free whole-genome bisulfite sequencing by post-bisulfite adaptor tagging publication-title: Nucleic Acids Res doi: 10.1093/nar/gks454 – volume: 161 start-page: 1437 year: 2015 end-page: 1452 ident: CR5 article-title: The transcriptome and DNA methylome landscapes of human primordial germ cells publication-title: Cell doi: 10.1016/j.cell.2015.05.015 – volume: 78 start-page: 852 year: 2008 end-page: 858 ident: CR32 article-title: The early human germ cell lineage does not express SOX2 during development or upon culture publication-title: Biol Reprod doi: 10.1095/biolreprod.107.066175 – volume: 511 start-page: 606 year: 2014 end-page: 610 ident: CR33 article-title: The DNA methylation landscape of human early embryos publication-title: Nature doi: 10.1038/nature13544 – volume: 529 start-page: 403 year: 2016 end-page: 407 ident: CR30 article-title: NANOG alone induces germ cells in primed epiblast by activation of enhancers publication-title: Nature doi: 10.1038/nature16480 – volume: 31 start-page: 4348 year: 2011 end-page: 4355 ident: CR26 article-title: Evolution of nucleosome occupancy: conservation of global properties and divergence of gene-specific patterns publication-title: Mol Cell Biol doi: 10.1128/MCB.05276-11 – volume: 7 start-page: 562 year: 2012 end-page: 578 ident: CR57 article-title: Differential gene and transcript expression analysis of RNA-seq experiments with TopHat and Cufflinks publication-title: Nat Protoc doi: 10.1038/nprot.2012.016 – volume: 24 start-page: 1421 year: 2014 end-page: 1432 ident: CR20 article-title: Reconfiguration of nucleosome-depleted regions at distal regulatory elements accompanies DNA methylation of enhancers and insulators in cancer publication-title: Genome Res doi: 10.1101/gr.163485.113 – volume: 6 start-page: 377 year: 2009 end-page: 382 ident: CR48 article-title: mRNA-Seq whole-transcriptome analysis of a single cell publication-title: Nat Methods doi: 10.1038/nmeth.1315 – volume: 12 start-page: 115 year: 2015 end-page: 121 ident: CR53 article-title: Orchestrating high-throughput genomic analysis with Bioconductor publication-title: Nat Methods doi: 10.1038/nmeth.3252 – volume: 10 start-page: e1004868 year: 2014 ident: CR51 article-title: Genome-wide analysis of DNA methylation dynamics during early human development publication-title: PLoS Genet doi: 10.1371/journal.pgen.1004868 – volume: 23 start-page: 616 year: 2013 end-page: 627 ident: CR21 article-title: High-resolution DNA methylome analysis of primordial germ cells identifies gender-specific reprogramming in mice publication-title: Genome Res doi: 10.1101/gr.148023.112 – volume: 7 start-page: 845 year: 2001 end-page: 852 ident: CR45 article-title: Germ cell specific expression of c-kit in the human fetal gonad publication-title: Mol Hum Reprod doi: 10.1093/molehr/7.9.845 – volume: 291 start-page: 4386 year: 2016 end-page: 4398 ident: CR35 article-title: Epigenomic landscape of human fetal brain, heart, and liver publication-title: J Biol Chem doi: 10.1074/jbc.M115.672931 – volume: 5 start-page: 516 year: 2010 end-page: 535 ident: CR49 article-title: RNA-Seq analysis to capture the transcriptome landscape of a single cell publication-title: Nat Protoc doi: 10.1038/nprot.2009.236 – volume: 18 start-page: 330 year: 2016 end-page: 340 ident: CR14 article-title: Complete meiosis from embryonic stem cell-derived germ cells publication-title: Cell Stem Cell doi: 10.1016/j.stem.2016.01.017 – volume: 25 start-page: 2078 year: 2009 end-page: 2079 ident: CR50 article-title: The Sequence Alignment/Map format and SAMtools publication-title: Bioinformatics doi: 10.1093/bioinformatics/btp352 – volume: 153 start-page: 737 year: 2013 end-page: 739 ident: CR4 article-title: Beyond DNA: programming and inheritance of parental methylomes publication-title: Cell doi: 10.1016/j.cell.2013.04.044 – volume: 161 start-page: 1425 year: 2015 end-page: 1436 ident: CR6 article-title: DNA demethylation dynamics in the human prenatal germline publication-title: Cell doi: 10.1016/j.cell.2015.05.012 – volume: 48 start-page: 849 year: 2012 end-page: 862 ident: CR22 article-title: The dynamics of genome-wide DNA methylation reprogramming in mouse primordial germ cells publication-title: Mol Cell doi: 10.1016/j.molcel.2012.11.001 – volume: 146 start-page: R125 year: 2013 end-page: 130 ident: CR43 article-title: Seeking the origin of female germline stem cells in the mammalian ovary publication-title: Reproduction doi: 10.1530/REP-13-0069 – volume: 25 start-page: 1727 year: 2015 end-page: 1738 ident: CR24 article-title: DNA-guided establishment of nucleosome patterns within coding regions of a eukaryotic genome publication-title: Genome Res doi: 10.1101/gr.188516.114 – volume: 9 start-page: e1003354 year: 2013 ident: CR37 article-title: Understanding the connection between epigenetic DNA methylation and nucleosome positioning from computer simulations publication-title: PLoS Comput Biol doi: 10.1371/journal.pcbi.1003354 – volume: 24 start-page: 260 year: 2014 end-page: 266 ident: CR23 article-title: Canonical nucleosome organization at promoters forms during genome activation publication-title: Genome Res doi: 10.1101/gr.157750.113 – volume: 488 start-page: 116 year: 2012 end-page: 120 ident: CR28 article-title: A map of the cis-regulatory sequences in the mouse genome publication-title: Nature doi: 10.1038/nature11243 – volume: 511 start-page: 611 year: 2014 end-page: 615 ident: CR34 article-title: DNA methylation dynamics of the human preimplantation embryo publication-title: Nature doi: 10.1038/nature13581 – volume: 528 start-page: 142 year: 2015 end-page: 146 ident: CR41 article-title: Genome-wide detection of DNase I hypersensitive sites in single cells and FFPE tissue samples publication-title: Nature – volume: 16 start-page: 517 year: 2015 end-page: 532 ident: CR16 article-title: Quantitative dynamics of chromatin remodeling during germ cell specification from mouse embryonic stem cells publication-title: Cell Stem Cell doi: 10.1016/j.stem.2015.03.002 – volume: 10 start-page: R25 year: 2009 ident: CR56 article-title: Ultrafast and memory-efficient alignment of short DNA sequences to the human genome publication-title: Genome Biol doi: 10.1186/gb-2009-10-3-r25 – volume: 5 start-page: R80 year: 2004 ident: CR54 article-title: Bioconductor: open software development for computational biology and bioinformatics publication-title: Genome Biol doi: 10.1186/gb-2004-5-10-r80 – volume: 146 start-page: 519 year: 2011 end-page: 532 ident: CR13 article-title: Reconstitution of the mouse germ cell specification pathway in culture by pluripotent stem cells publication-title: Cell doi: 10.1016/j.cell.2011.06.052 – volume: 501 start-page: 222 year: 2013 end-page: 226 ident: CR15 article-title: Induction of mouse germ-cell fate by transcription factors publication-title: Nature doi: 10.1038/nature12417 – volume: 22 start-page: 2497 year: 2012 end-page: 2506 ident: CR19 article-title: Genome-wide mapping of nucleosome positioning and DNA methylation within individual DNA molecules publication-title: Genome Res doi: 10.1101/gr.143008.112 – volume: 110 start-page: 16061 year: 2013 ident: BFcr2016128_CR8 publication-title: Proc Natl Acad Sci USA doi: 10.1073/pnas.1315204110 – volume: 23 start-page: 2126 year: 2013 ident: BFcr2016128_CR44 publication-title: Genome Res doi: 10.1101/gr.161679.113 – volume: 459 start-page: 108 year: 2009 ident: BFcr2016128_CR29 publication-title: Nature doi: 10.1038/nature07829 – volume: 11 start-page: 817 year: 2014 ident: BFcr2016128_CR47 publication-title: Nat Methods doi: 10.1038/nmeth.3035 – volume: 18 start-page: 330 year: 2016 ident: BFcr2016128_CR14 publication-title: Cell Stem Cell doi: 10.1016/j.stem.2016.01.017 – volume: 146 start-page: 519 year: 2011 ident: BFcr2016128_CR13 publication-title: Cell doi: 10.1016/j.cell.2011.06.052 – volume: 134 start-page: 2627 year: 2007 ident: BFcr2016128_CR18 publication-title: Development doi: 10.1242/dev.005611 – volume: 12 start-page: 115 year: 2015 ident: BFcr2016128_CR53 publication-title: Nat Methods doi: 10.1038/nmeth.3252 – volume: 529 start-page: 403 year: 2016 ident: BFcr2016128_CR30 publication-title: Nature doi: 10.1038/nature16480 – volume: 278 start-page: 440 year: 2005 ident: BFcr2016128_CR17 publication-title: Dev Biol doi: 10.1016/j.ydbio.2004.11.025 – volume: 5 start-page: 516 year: 2010 ident: BFcr2016128_CR49 publication-title: Nat Protoc doi: 10.1038/nprot.2009.236 – volume: 488 start-page: 116 year: 2012 ident: BFcr2016128_CR28 publication-title: Nature doi: 10.1038/nature11243 – volume: 24 start-page: 1421 year: 2014 ident: BFcr2016128_CR20 publication-title: Genome Res doi: 10.1101/gr.163485.113 – volume: 160 start-page: 253 year: 2015 ident: BFcr2016128_CR31 publication-title: Cell doi: 10.1016/j.cell.2014.12.013 – volume: 146 start-page: R125 year: 2013 ident: BFcr2016128_CR43 publication-title: Reproduction doi: 10.1530/REP-13-0069 – volume: 24 start-page: 260 year: 2014 ident: BFcr2016128_CR23 publication-title: Genome Res doi: 10.1101/gr.157750.113 – volume: 511 start-page: 611 year: 2014 ident: BFcr2016128_CR34 publication-title: Nature doi: 10.1038/nature13581 – volume: 16 start-page: 517 year: 2015 ident: BFcr2016128_CR16 publication-title: Cell Stem Cell doi: 10.1016/j.stem.2015.03.002 – volume: 6 start-page: 377 year: 2009 ident: BFcr2016128_CR48 publication-title: Nat Methods doi: 10.1038/nmeth.1315 – volume: 511 start-page: 606 year: 2014 ident: BFcr2016128_CR33 publication-title: Nature doi: 10.1038/nature13544 – volume: 24 start-page: 324 year: 2013 ident: BFcr2016128_CR10 publication-title: Dev Cell doi: 10.1016/j.devcel.2012.12.011 – volume: 338 start-page: 971 year: 2012 ident: BFcr2016128_CR12 publication-title: Science doi: 10.1126/science.1226889 – volume: 348 start-page: 910 year: 2015 ident: BFcr2016128_CR40 publication-title: Science doi: 10.1126/science.aab1601 – volume: 132 start-page: 311 year: 2008 ident: BFcr2016128_CR27 publication-title: Cell doi: 10.1016/j.cell.2007.12.014 – volume: 291 start-page: 4386 year: 2016 ident: BFcr2016128_CR35 publication-title: J Biol Chem doi: 10.1074/jbc.M115.672931 – volume: 139 start-page: 15 year: 2012 ident: BFcr2016128_CR2 publication-title: Development doi: 10.1242/dev.050849 – volume: 10 start-page: R25 year: 2009 ident: BFcr2016128_CR56 publication-title: Genome Biol doi: 10.1186/gb-2009-10-3-r25 – volume: 25 start-page: 1727 year: 2015 ident: BFcr2016128_CR24 publication-title: Genome Res doi: 10.1101/gr.188516.114 – volume: 25 start-page: 2078 year: 2009 ident: BFcr2016128_CR50 publication-title: Bioinformatics doi: 10.1093/bioinformatics/btp352 – volume: 22 start-page: 2497 year: 2012 ident: BFcr2016128_CR19 publication-title: Genome Res doi: 10.1101/gr.143008.112 – volume: 161 start-page: 1437 year: 2015 ident: BFcr2016128_CR5 publication-title: Cell doi: 10.1016/j.cell.2015.05.015 – volume: 23 start-page: 616 year: 2013 ident: BFcr2016128_CR21 publication-title: Genome Res doi: 10.1101/gr.148023.112 – volume: 10 start-page: e1004868 year: 2014 ident: BFcr2016128_CR51 publication-title: PLoS Genet doi: 10.1371/journal.pgen.1004868 – volume: 528 start-page: 142 year: 2015 ident: BFcr2016128_CR41 publication-title: Nature doi: 10.1038/nature15740 – volume: 38 start-page: 576 year: 2010 ident: BFcr2016128_CR52 publication-title: Mol Cell doi: 10.1016/j.molcel.2010.05.004 – volume: 161 start-page: 1453 year: 2015 ident: BFcr2016128_CR7 publication-title: Cell doi: 10.1016/j.cell.2015.04.053 – volume: 7 start-page: 562 year: 2012 ident: BFcr2016128_CR57 publication-title: Nat Protoc doi: 10.1038/nprot.2012.016 – volume: 474 start-page: 516 year: 2011 ident: BFcr2016128_CR38 publication-title: Nature doi: 10.1038/nature10002 – volume: 10 start-page: 1213 year: 2013 ident: BFcr2016128_CR42 publication-title: Nat Methods doi: 10.1038/nmeth.2688 – volume: 501 start-page: 222 year: 2013 ident: BFcr2016128_CR15 publication-title: Nature doi: 10.1038/nature12417 – volume: 466 start-page: 388 year: 2010 ident: BFcr2016128_CR36 publication-title: Nature doi: 10.1038/nature09147 – volume: 40 start-page: e136 year: 2012 ident: BFcr2016128_CR46 publication-title: Nucleic Acids Res doi: 10.1093/nar/gks454 – volume: 23 start-page: 257 year: 2007 ident: BFcr2016128_CR55 publication-title: Bioinformatics doi: 10.1093/bioinformatics/btl567 – volume: 18 start-page: 721 year: 2016 ident: BFcr2016128_CR11 publication-title: Cell Stem Cell doi: 10.1016/j.stem.2016.05.001 – volume: 5 start-page: R80 year: 2004 ident: BFcr2016128_CR54 publication-title: Genome Biol doi: 10.1186/gb-2004-5-10-r80 – volume: 78 start-page: 852 year: 2008 ident: BFcr2016128_CR32 publication-title: Biol Reprod doi: 10.1095/biolreprod.107.066175 – volume: 48 start-page: 849 year: 2012 ident: BFcr2016128_CR22 publication-title: Mol Cell doi: 10.1016/j.molcel.2012.11.001 – volume: 14 start-page: 710 year: 2014 ident: BFcr2016128_CR1 publication-title: Cell Stem Cell doi: 10.1016/j.stem.2014.05.008 – volume: 161 start-page: 1425 year: 2015 ident: BFcr2016128_CR6 publication-title: Cell doi: 10.1016/j.cell.2015.05.012 – volume: 132 start-page: 887 year: 2008 ident: BFcr2016128_CR25 publication-title: Cell doi: 10.1016/j.cell.2008.02.022 – volume: 9 start-page: e1003354 year: 2013 ident: BFcr2016128_CR37 publication-title: PLoS Comput Biol doi: 10.1371/journal.pcbi.1003354 – volume: 7 start-page: 845 year: 2001 ident: BFcr2016128_CR45 publication-title: Mol Hum Reprod doi: 10.1093/molehr/7.9.845 – volume: 153 start-page: 737 year: 2013 ident: BFcr2016128_CR4 publication-title: Cell doi: 10.1016/j.cell.2013.04.044 – volume: 523 start-page: 486 year: 2015 ident: BFcr2016128_CR39 publication-title: Nature doi: 10.1038/nature14590 – volume: 3 start-page: 1777 year: 2013 ident: BFcr2016128_CR9 publication-title: Cell Rep doi: 10.1016/j.celrep.2013.04.032 – volume: 16 start-page: 6 year: 2002 ident: BFcr2016128_CR3 publication-title: Genes Dev doi: 10.1101/gad.947102 – volume: 31 start-page: 4348 year: 2011 ident: BFcr2016128_CR26 publication-title: Mol Cell Biol doi: 10.1128/MCB.05276-11 – reference: 25042786 - Nat Methods. 2014 Aug;11(8):817-20 – reference: 21820164 - Cell. 2011 Aug 19;146(4):519-32 – reference: 22960375 - Genome Res. 2012 Dec;22(12):2497-506 – reference: 11517291 - Mol Hum Reprod. 2001 Sep;7(9):845-52 – reference: 15461798 - Genome Biol. 2004;5(10):R80 – reference: 25633503 - Nat Methods. 2015 Feb;12(2):115-21 – reference: 23727241 - Cell Rep. 2013 Jun 27;3(6):1777-84 – reference: 25501653 - PLoS Genet. 2014 Dec 11;10(12):e1004868 – reference: 15680362 - Dev Biol. 2005 Feb 15;278(2):440-58 – reference: 18243105 - Cell. 2008 Jan 25;132(2):311-22 – reference: 22649061 - Nucleic Acids Res. 2012 Sep 1;40(17):e136 – reference: 23219530 - Mol Cell. 2012 Dec 28;48(6):849-62 – reference: 24278005 - PLoS Comput Biol. 2013;9(11):e1003354 – reference: 21896781 - Mol Cell Biol. 2011 Nov;31(21):4348-55 – reference: 25800778 - Cell Stem Cell. 2015 May 7;16(5):517-32 – reference: 24179143 - Genome Res. 2013 Dec;23(12):2126-35 – reference: 26330564 - Genome Res. 2015 Nov;25(11):1727-38 – reference: 25543152 - Cell. 2015 Jan 15;160(1-2):253-68 – reference: 26605532 - Nature. 2015 Dec 3;528(7580):142-6 – reference: 25079558 - Nature. 2014 Jul 31;511(7511):611-5 – reference: 27257761 - Cell Stem Cell. 2016 Jun 2;18(6):721-35 – reference: 22383036 - Nat Protoc. 2012 Mar 01;7(3):562-78 – reference: 22763441 - Nature. 2012 Aug 2;488(7409):116-20 – reference: 26083756 - Nature. 2015 Jul 23;523(7561):486-90 – reference: 26046443 - Cell. 2015 Jun 4;161(6):1437-52 – reference: 18329373 - Cell. 2008 Mar 7;132(5):887-98 – reference: 22147951 - Development. 2012 Jan;139(1):15-31 – reference: 17098774 - Bioinformatics. 2007 Jan 15;23(2):257-8 – reference: 19349980 - Nat Methods. 2009 May;6(5):377-82 – reference: 23410886 - Genome Res. 2013 Apr;23(4):616-27 – reference: 23663772 - Cell. 2013 May 9;153(4):737-9 – reference: 19261174 - Genome Biol. 2009;10(3):R25 – reference: 24097267 - Nat Methods. 2013 Dec;10(12):1213-8 – reference: 26004067 - Cell. 2015 Jun 4;161(6):1425-36 – reference: 24043772 - Proc Natl Acad Sci U S A. 2013 Oct 1;110(40):16061-6 – reference: 26719341 - J Biol Chem. 2016 Feb 26;291(9):4386-98 – reference: 24285721 - Genome Res. 2014 Feb;24(2):260-6 – reference: 23352811 - Dev Cell. 2013 Feb 11;24(3):324-33 – reference: 19295514 - Nature. 2009 May 7;459(7243):108-12 – reference: 25079557 - Nature. 2014 Jul 31;511(7511):606-10 – reference: 26046444 - Cell. 2015 Jun 4;161(6):1453-67 – reference: 18199879 - Biol Reprod. 2008 May;78(5):852-8 – reference: 20512117 - Nature. 2010 Jul 15;466(7304):388-92 – reference: 21602827 - Nature. 2011 May 22;474(7352):516-20 – reference: 11782440 - Genes Dev. 2002 Jan 1;16(1):6-21 – reference: 19505943 - Bioinformatics. 2009 Aug 15;25(16):2078-9 – reference: 26751055 - Nature. 2016 Jan 21;529(7586):403-7 – reference: 24905162 - Cell Stem Cell. 2014 Jun 5;14(6):710-9 – reference: 20513432 - Mol Cell. 2010 May 28;38(4):576-89 – reference: 17567665 - Development. 2007 Jul;134(14):2627-38 – reference: 20203668 - Nat Protoc. 2010 Mar;5(3):516-35 – reference: 23801781 - Reproduction. 2013 Aug 12;146(4):R125-30 – reference: 26923202 - Cell Stem Cell. 2016 Mar 3;18(3):330-40 – reference: 24916973 - Genome Res. 2014 Sep;24(9):1421-32 – reference: 25953818 - Science. 2015 May 22;348(6237):910-4 – reference: 23913270 - Nature. 2013 Sep 12;501(7466):222-6 – reference: 23042295 - Science. 2012 Nov 16;338(6109):971-5 |
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| Snippet | Chromatin remodeling is important for the epigenetic reprogramming of human primordial germ cells. However, the comprehensive chromatin state has not yet been... |
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| SubjectTerms | 631/136/532/2435 631/208/176/1988 631/337/100 692/698/690/1520 Animals Biomedical and Life Sciences Cell Biology Cellular Reprogramming - genetics Chromatin - metabolism Deoxyribonucleic acid DNA DNA Methylation - genetics DNA甲基化 Fetus - cytology Genomic Imprinting Germ Cells - metabolism Humans Life Sciences Male Mammals - genetics Mice Nucleosomes - metabolism Organ Specificity - genetics Original original-article Promoter Regions, Genetic - genetics Repetitive Sequences, Nucleic Acid - genetics 原始生殖细胞 可及性 小鼠 染色质重塑 生殖细胞发育 胚胎生殖细胞 表观遗传 |
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| Title | DNA methylation and chromatin accessibility profiling of mouse and human fetal germ cells |
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