Mitochondrial NADH Kinase, Pos5p, Is Required for Efficient Iron-Sulfur Cluster Biogenesis in Saccharomyces cerevisiae

• Published in: The Journal of biological chemistry Vol. 285; no. 50; pp. 39409 - 39424
• Main Authors: Pain, Jayashree, Balamurali, M.M., Dancis, Andrew, Pain, Debkumar
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 10.12.2010; American Society for Biochemistry and Molecular Biology
• Subjects: Aconitate Hydratase - metabolism; Dose-Response Relationship, Drug; Ferredoxins - chemistry; Gene Expression Regulation, Fungal; Iron Metabolism; Iron-Sulfur Protein; Iron-Sulfur Proteins - chemistry; Kinetics; Metabolism; Metals; Mitochondria; Mitochondria - enzymology; Mitochondria - metabolism; Mitochondrial Metabolism; Mitochondrial Proteins - metabolism; Models, Genetic; NAD - chemistry; Oxidative Stress; Oxygen - chemistry; Phosphotransferases (Alcohol Group Acceptor) - metabolism; Saccharomyces cerevisiae; Saccharomyces cerevisiae - genetics; Saccharomyces cerevisiae Proteins - metabolism; Sulfur - metabolism; Yeast; Mitochondria; Iron-Sulfur Protein; Yeast; Iron Metabolism; Metals; Mitochondrial Metabolism

In Saccharomyces cerevisiae, the mitochondrial inner membrane readily allows transport of cytosolic NAD+, but not NADPH, to the matrix. Pos5p is the only known NADH kinase in the mitochondrial matrix. The enzyme phosphorylates NADH to NADPH and is the major source of NADPH in the matrix. The importa...